anti-Myelin mouse monoclonal, 2B5, ascites fluid
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgM
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Human myelin isolated from peripheral nervous system|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:10|
|Immunohistochemistry (IHC) - paraffin||1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
In paraffin sections antibody pm 43 recognizes only myelin in the peripheral nervous system (PNS). In frozen sections it also reacts with myelin in the central nervous system (CNS). Is useful for studying myelination and demyelination processes in the PNS, and remyelination processes in the CNS as can be observed after trauma in multiple sclerosis. Reacts with a 43 kD protein in immunoblotting.
Positive control: Peripheral nerve.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.