Disease related

anti-Myosin mouse monoclonal, 414.0, supernatant

Cat. No: 10605

Available, delivery time 1-7 days

$331.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
  • Reacts with chicken, human, mouse and rabbit
  • Isotype: IgM
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgM
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Chicken, Human, Mouse, Rabbit
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 414.0
Immunogen Chicken skeletal muscle cell preparation
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:5-1:10
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains.
It is not reactive with alpha-cardiac myosin.

Positive control: muscle, myofibrils (chicken)

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.