anti-Myosin mouse monoclonal, 414.0, supernatant
- Mouse monoclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with chicken, human, mouse and rabbit
- Isotype: IgM
|Application||ICC/IF, IHC, WB|
|Purification||Hybridoma cell culture supernatant|
|Reactivity||Chicken, Human, Mouse, Rabbit|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Chicken skeletal muscle cell preparation|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:5-1:10|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
|Immunohistochemistry (IHC) - paraffin||1:5-1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common site-chain), no reaction with light chains.
It is not reactive with alpha-cardiac myosin.
Positive control: muscle, myofibrils (chicken)
Q & A's
0 of 0 reviews
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.