anti-Na+/I- Symporter (rat) rabbit polyclonal
- Rabbit polyclonal
- Suitable for ICC/IF and IHC on frozen sections
- Reacts with human, pig and rat
|Quantity||100 µl (lyoph.)|
|Reactivity||Human, Pig, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide from rat NIS|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|UniprotID||Q92911 (Human), A0A287AIH3 (Pig), Q63008 (Rat)|
|Synonym||Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5, SLC5A5, NIS|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:1,200-1:2,000|
|Immunohistochemistry (IHC) - frozen||1:1,200-1:2,000|
Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane. The antiserum is raised against the C-terminus of rat NIS. Suitable for studying the sensitivity of thyroid tumors to iodine treatment. Absorption with 50-100 ug immunogen per ml diluted antiserum abolishes staining.
Positive control: frozen sections of rat thyroid.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.