Disease related

anti-Neurofilaments (NF-L) mouse monoclonal, NR4, purified

Cat. No: 691713

Available, delivery time 1-7 days


Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, cat, chicken, gerbil, human, mouse, pig and rat
  • Isotype: IgG1 kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1 kappa
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Cat, Chicken, Gerbil, Human, Mouse, Pig, Rat
Storage 2-8°C
Intended use Research use only
Clone NR4
Immunogen Crude neurofilament preparation from porcine spinal cord, containing the triplet polypeptides of 200, 145 and 68 kDa
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
Western Blot (WB) 1:100-1:200 (0.5-1 µg/ml)

NR4 reacts specifically with the 68 kDa subunit (NF-L) present in all neurofilaments. Neuronal intermediate filaments are typically referred to as a neurofilament triplet of low (L), middle (M) and high (H) molecular weight subunits of 68 kDa, 150 kDa and 200 kDa, respectively. Neurofilaments constitute the main structural elements of neuronal axons and dendrites.

Positive control: brain, neuroblastoma.

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