anti-Nitric Oxide Synthase (C-terminus) guinea pig polyclonal, serum
- Guinea pig polyclonal
- Suitable for IHC on frozen sections
- Reacts with cat, dog, human and rat
|Quantity||50 µl (lyoph.)|
|Reactivity||Cat, Dog, Human, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide from the C-terminus of rat cerebellar NO-synthase, coupled to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|UniprotID||A0A2I2UYT7 (Cat, Felis silvestris catus), O62699 (Dog, Canis familiaris), P35228 (Human), Q06518 (Rat)|
|Synonym||Nitric oxide synthase, inducible, EC 184.108.40.206, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:1,500-1:2,500|
Nitric oxide synthase is an enzyme catalizing the synthesis of NO from L-arginine. The antiserum was raised against a synthetic peptide of rat cerebellar NOS, that shows no homology with other related proteins.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining.
Positive control: frozen sections of rat colon.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
- Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
- Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).
- Air-drying of the section.
- Block with the serum of the species in which the secondary antibody was raised for 30 min.
- Incubation with 1st antibody 1 h at RT in moist chamber.
- Wash 3x with PBS.
- Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
- Wash 3x with PBS.
- Immerse shortly into ethanol.
- Let air dry.
- Cover with mounting medium.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.