Epitope mapped
Disease related

anti-Nucleolar Protein NO38 mouse monoclonal, No-63, ascites fluid

Supernatant

Ascites fluid

Cat. No: 612123

Available, delivery time 1-7 days

$271.00*

Key Features
  • Ascites fluid
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG2a
Product description
Quantity 250 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application IHC, IP, WB
Purification Ascites
Reactivity Chicken, Pleurodeles, Triturus, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone No-63
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Formulation Contains 0.09% sodium azide
UniprotID
Synonym Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:300-1:500 (also after fixation with paraformaldehyde)
Immunoprecipitation (IP) Assay dependent (from Xenopus oocyte extracts nucleoplasmin - a 30 kDa protein - is co-precipitated, which shows high sequence homology to NO38)
Western Blot (WB) 1:1,000-1:2,000 (for blotting transfer nitrocellulose sheets are superior to nylon sheets)
Background
Recognizes a 38 kDa nucleolar protein (NO38) in Xenopus (also termed numatrin, B23, nucleophosmin, in other species). No-63 stains specifically the granular component of the nucleolus and small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes.
References/Publications (3)
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anti-Nucleolar Protein NO38 mouse monoclonal, No-185, supernatant concentrate
Cat. No: 652125
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with chicken, pleurodelis, triturus and xenopus
  • Isotype: IgG1

$339.00*