anti-Nucleolar Protein xNopp180 mouse monoclonal, No-114, supernatant

Cat. No: 651124

Available, delivery time 1-7 days

$245.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with xenopus
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, IP
Purification Hybridoma cell culture supernatant
Reactivity Xenopus
No reactivity Chicken, Mouse, Pleurodelis, Rana, Rat, Triturus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone No-114
Immunogen Nucleolar fraction prepared from Xenopus laevis oocytes
Formulation Contains 0.09% sodium azide
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5-1:10 (also after fixation with paraformaldehyde)
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:50
Background

The antibody stains specifically the nucleolus and Cajal bodies in interphase cells. The antigen is a constitutively expressed nucleolar component and a specific marker for the dense fibrillar component of the interphase nucleolus. The protein recognized is a 180 kDa polypeptide in Xenopus (xNopp180), homologue to the rat polypeptide (Nopp140). When applied at high concentration to human cells the antibody reacts with a nucleolar component which, however, does not correspond to xNopp180.

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