anti-Nucleoplasmic Protein AND-1 mouse monoclonal, 23-5-14, supernatant concentrate
- Mouse monoclonal
- Suitable for IHC, IP and WB
- Reacts with xenopus
- Isotype: IgG1
|Application||IHC, IP, WB|
|Purification||Hybridoma cell culture supernatant concentrate|
|No reactivity||Higher vertebrates|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Nuclear fraction prepared from Xenopus laevis oocytes|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:300-1:500 (fixation with paraformaldehyde recommended)|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:3,000|
The antibody recognizes a 125 kDa nucleoplasmic protein (AND-1, acidic nucleoplasmic DNA-binding protein) in Xenopus.
Immunolocalization studies showed a dispersed, finely granular distribution pattern throughout the nucleoplasm.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.