Highly published

anti-Perilipin 1 (N-terminus) guinea pig polyclonal, serum, sample (ready-to-use)

Serum

Sample

Cat. No: GP29S

Available, delivery time 1-7 days

$65.00*

Key Features
  • Stabilized antiserum, ready-to-use for IHC
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with mouse and rat
Product description
Quantity 600 µl (ready-to-use)
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Stabilized antiserum
Reactivity Mouse, Rat
No reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Duplicated N-terminus of mouse/ rat perilipin / PLIN1 (aa 1-20); MSMNKGPTLLDGDLPEQENV-C
Formulation Contains 0.09% sodium azide
UniprotID Q8CGN5 (Mouse),P43884 (Rat)
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kDa, respectively. The N-terminus, however, remains unchanged.
The antiserum reacts specifically with PLIN1 (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.
It also is a useful pathological marker since PLIN1 becames expressed de novo in hepatocyte steatogenesis.
The antiserum does not cross-react with ADRP (adipophilin, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5).


References/Publications (33)
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Size
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Category Protocol
Size 917.17 KB
Filetype pdf
Category Protocol
Size 919.99 KB
Filetype pdf
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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