Highly published

anti-Perilipin 1 (N-terminus) guinea pig polyclonal, serum

Cat. No: GP29

Prices excl. tax

Available, delivery time 1-7 days

$344.00*

Datasheet

Key Features

Stabilized antiserum
Guinea pig polyclonal
Suitable for IHC and WB
Reacts with mouse and rat

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	IHC, WB
Purification	Stabilized antiserum
Reactivity	Mouse, Rat
No reactivity	Human
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Duplicated N-terminus of mouse/ rat perilipin / PLIN1 (aa 1-20); MSMNKGPTLLDGDLPEQENV-C
Formulation	Contains 0.09% sodium azide
UniprotID	Q8CGN5 (Mouse), P43884 (Rat)
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100-1:500
Immunohistochemistry (IHC) - paraffin	1:100-1:500 (microwave treatment recommended)
Western Blot (WB)	1:1,000-1:2,000

Background

Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 and 46 kDa, respectively. The N-terminus, however, remains unchanged.
The antiserum reacts specifically with PLIN1 (A and B) located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells.
It also is a useful pathological marker since PLIN1 becames expressed de novo in hepatocyte steatogenesis.
The antiserum does not cross-react with ADRP (adipophilin, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5).

References/Publications (38)

Publication

Species

Application

Publication Chika Suzuki, Takako Komiya, Hana Inoue, Takayuki Yoshimoto, Hajime Matsumura. Adding collagen to adipose tissue transplant increases engraftment by promoting cell proliferation, neovascularisation and macrophage activity in a rat model.,

Species rat

Application IHC

Publication Moroki, T. et al. Databases for technical aspects of immunohistochemistry: 2021 update. J Toxicol Pathol. 34, 161-180(2021).

Species rat

Application IHC

Publication Liu, K. et al. Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix. Mater. Today Bio 12, 100161 (2021).

Species Mouse

Application IHC-P-IF

Publication Zhou, H. et al. Berardinelli-Seip congenital lipodystrophy 2/SEIPIN determines brown adipose tissue maintenance and thermogenic programing. Mol.Metab. 36, 100971 (2020)

Species mouse

Application WB

Publication Müller, G. et al. CB1 Receptor-Dependent and Independent Induction of Lipolysis in Primary Rat Adipocytes by the Inverse Agonist Rimonabant (SR141716A). Molecules. 25, (2020)

Species rat

Application WB

Publication Mizunoe, Y. et al. Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Sci.Rep. 10, 634 (2020)

Species mouse

Application IHC (paraffin)

Publication Wang, S. et al. Molecular Mechanisms of Adipose Tissue Survival during Severe Hypoxia: Implications for Autologous Fat Graft Performance. Plast.Reconstr.Surg.Glob.Open. 7, e2275 (2019)

Species human

Application IHC (paraffin)

Publication Craft, C. et al. Bone marrow adipose tissue does not express UCP1 during development or adrenergic-induced remodeling. Sci.Rep. 9, 17427 (2019)

Species mouse

Application IHC (frozen)

Publication Robles, H. et al. Characterization of the bone marrow adipocyte niche with three-dimensional electron microscopy. Bone. 118, 89-98 (2019)

Species mouse

Application IHC-IF (frozen)

Publication Zhou, H. et al. Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy. JCI.Insight. 5, (2019)

Species mouse

Application WB

Publication Oyama, Y. et al. Colon adenoma and adenocarcinoma with clear cell components - two case reports. Diagn.Pathol. 14, 37 (2019)

Species human

Application IHC

Publication He, Y. et al. Human adipose liquid extract induces angiogenesis and adipogenesis: a novel cell-free therapeutic agent. Stem.Cell.Res.Ther. 10, 252 (2019)

Species human

Application IHC (paraffin)

Publication Tang, J. et al. Obesity-associated family with sequence similarity 13, member A (FAM13A) is dispensable for adipose development and insulin sensitivity. Int.J.Obes.(Lond). , (2018)

Species human,mouse

Application WB

Publication Lehti, S. et al. Extracellular Lipids Accumulate in Human Carotid Arteries as Distinct Three-Dimensional Structures and Have Proinflammatory Properties. Am.J.Pathol. 188, 525-538 (2018)

Species human

Application WB

Publication Furukawa, S., Nagaike, M. & Ozaki, K. Databases for technical aspects of immunohistochemistry. J. Toxicol. Pathol. 30, 79-107 (2017).

Species rat

Application IHC (paraffin)

Publication Takahashi, Y. et al. Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells. EBioMedicine. 23, 34-45 (2017).

Species mouse

Application IHC-IF (paraffin)

Publication Wang, L. et al. Podocyte-specific knockout of cyclooxygenase 2 exacerbates diabetic kidney disease. Am.J.Physiol.Renal.Physiol. 313, F430-F439 (2017)

Species mouse

Application IHC (paraffin)

Publication Bialesova, L. et al. Epigenetic Regulation of PLIN 1 in Obese Women and its Relation to Lipolysis. Sci.Rep. 7, 10152 (2017).

Species human

Application WB

Publication Barquissau, V. et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol. Metab. 5, 352-365 (2016).

Species human

Application WB

Publication Inoue, J. et al. Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6, 32750 (2016).

Species mouse

Application WB

Publication Gallardo-Montejano, V. I. et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1a/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat. Commun. 7, (2016).

Species mouse

Application WB

Publication Frisdal, E. et al. Adipocyte ATP-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth, and Human Obesity. Diabetes 64, 840-855 (2015).

Species mouse

Application WB,IHC (paraffin)

Publication Kozusko, K. et al. Clinical and molecular characterization of a novel PLIN1 frameshift mutation identified in patients with familial partial lipodystrophy Europe PMC Funders Group. Diabetes 64, 299-310 (2015).

Species human,mouse

Application WB

Publication Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).

Species human

Application WB,ICC-IF

Publication Mcdonough, P. M. et al. Differential Phosphorylation of Perilipin 1A at the Initiation of Lipolysis Revealed by Novel Monoclonal Antibodies and High Content Analysis. PLoS One 8, (2013).

Species mouse

Application ICC-IF

Publication Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim. Biophys. Acta 1830, 4642-4649 (2013).

Species human

Application IHC (paraffin)

Publication Stenson, B. M. et al. Liver X Receptor (LXR) Regulates Human Adipocyte Lipolysis. J. Biol. Chem. 286, 370-379 (2011).

Species human

Application WB

Publication Straub, B. K. et al. Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod. Pathol. 23, 480-492 (2010).

Species human

Application WB,IHC

Publication Buers, I. et al. TIP47, a Lipid Cargo Protein Involved in Macrophage Triglyceride Metabolism. Arterioscler. Thromb. Vasc. Biol. 29, 767-773 (2009).

Species human

Application WB,ICC-IF

Publication Alsted, T. J. et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training. Am. J. Physiol. - Endocrinol. Metab. 296, E445-E453 (2008).

Species human

Application WB

Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008).

Species human

Application WB,IHC

Publication Yamaguchi, T., Omatsu, N., Omukae, A. & Osumi, T. Analysis of interaction partners for perilipin and ADRP on lipid droplets*. Mol. Cell. Biochem. 284, 167-173 (2006).

Species mouse

Application ICC-IF

Publication Muthusamy, K., Halbert, G. & Roberts, F. Immunohistochemical staining for adipophilin, perilipin and TIP47. J. Clin. Pathol. 59, 1166-1170 (2006).

Species human

Application IHC (paraffin)

Publication Roepstorff, C. et al. Sex differences in hormone-sensitive lipase expression, activity, and phosphorylation in skeletal muscle at rest and during exercise. Am. J. Physiol. - Endocrinol. Metab. 291, E1106-E1114 (2006).

Species human

Application WB

Publication Yamaguchi, T., Matsushita, S., Motojima, K., Hirose, F. & Osumi, T. MLDP, a novel PAT family protein localized to lipid droplets and enriched in the heart, is regulated by peroxisome proliferator-activated receptor alpha. J. Biol. Chem. 281, 14232-40 (20

Species mouse

Application WB,ICC-IF

Publication Robenek, H. et al. Lipid Droplets Gain PAT Family Proteins by Interaction with Specialized Plasma Membrane Domains*. J. Biol. Chem. 280, 26330-26338 (2005).

Species human

Application IEM

Publication Forcheron, F. et al. Genes of Cholesterol Metabolism in Human Atheroma: Overexpression of Perilipin and Genes Promoting Cholesterol Storage and Repression of ABCA1 Expression. Arterioscler. Thromb. Vasc. Biol. 25, 1711-1717 (2005).

Species human,rat

Application WB,IHC (paraffin)

Publication Ryden, M. et al. Targets for TNF-?-induced lipolysis in human adipocytes. Biochem. Biophys. Res. Commun. 318, 168-175 (2004).

Species human

Application WB

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File IHC guinea pig antibody

Category Protocol

Size 167.92 KB

Filetype pdf

File WB protocol

Category Protocol

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Filetype pdf

Datasheet

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FAQs

PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
Use the antibody in a higher dilution, but prolong incubation time and exposure time.
Always use a fresh aliquot of the antibody.
Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
Include an additional washing step.
You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
Always use a fresh aliquot of secondary antibody.
In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

3T3-L1 after stimulation
Adipose tissue
Steatosis liver
Atherosclerotic plaque material

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Tissue with a high fat content might be problematic in electrophoretic separation. One has to load much material onto the SDS gel in order to get enough protein for a positive immunological signal. In addition the protein solubility is sometimes hindered by high fat concentration in the sample.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

Progen Support

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