IHC analysis of human cutaneous skin using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
IHC analysis of human adipocytes in white adipose tissue of tumor microenvironment using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
IHC analysis of human cutaneous skin using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
IHC analysis of human adipocytes in white adipose tissue of tumor microenvironment using anti-Perilipin 1 (N-terminus) antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 1 (N-terminus), PERI 112.17 (Cat. No. 690156) was diluted in PBS (antibody concentration 500 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 10x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).
Key Features
- Mouse monoclonal
- Suitable for ICC/IF and IHC
- Reacts with human and mouse
- Isotype: IgG1
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC |
| Purification | Affinity chromatography |
| Reactivity | Human, Mouse |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | PERI 112.17 |
| Immunogen | Synthetic peptide of perilipin / PLIN1 (duplicated N-terminus of perilipin; aa 1-20) |
| Concentration | 100 µg/ml (20 µg) |
| Formulation | PBS buffer, pH 7.4 with 0.09% sodium azide and 0.5% BSA |
| UniprotID | O60240 (Human), Q8CGN5 (Mouse) |
| Synonym | Perilipin-1, Lipid droplet-associated protein, PLIN1, PERI, PLIN |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:200 (0.5 µg/ml) |
| Immunohistochemistry (IHC) - paraffin | 1:200 (0.5 µg/ml, microwave treatment recommended) |
Background
Perilipins build a family of phosphoproteins. The predominant forms in adipocytes, PLIN1 A and B arise by alternative RNA splicing from a single gene, generating polypeptides of 57 kDa and 46 kDa, respectively. The N-terminus, however, remains unchanged.
The antibody reacts specifically with all PLIN1 variants located at the surface of intracellular storage lipid droplets present e.g. in the adrenal gland, sebaceous gland, adipocytes of white and brown adipose tissue and cultured cells such as 3T3-L1 adipocytes and cultured steroidogenic adrenal cortical and Leydig cells. It is also a useful pathological marker for steatogenesis e.g. in liver. It does not cross-react with adipophilin (ADRP, also named PLIN2) or TIP47 (also named PLIN3) proteins (or additional members of the PLIN/PAT-family, e.g. MLDP or OXPAT/PAT-1, also named PLIN5 or LSDP5).
Tested reactivity on cultured cell lines: several human carcinoma cell lines; 3T3-L1 adipocytes.
References/Publications (8)
Publication
Species
Application
Species
Mouse
Application
PLA
Species
rat
Application
IHC (frozen)/IF
Species
human
Application
IHC (paraffin)
Species
human
Application
IHC (paraffin)
Species
human
Application
WB,ICC-IF
Species
mouse
Application
IHC (paraffin)
Species
human
Application
IHC
Downloads
File
Category
Size
Filetype
Customer Reviews
Login
FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:
- Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
- Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
- Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
- Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Related Products
anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, liquid, purified, sample
$117.00*
Cat. No: 690102S
Quantity:
200 µl (50 µg/ml)
anti-Perilipin 1 (N-terminus) mouse monoclonal, PERI 112.17, liquid, purified, sample
$120.00*
Cat. No: 690156S
Quantity:
200 µl (100 µg/ml)
