Disease related
Highly published

anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125, lyophilized, purified

Liquid

Liquid

Lyophilized

Cat. No: 610102

Available, delivery time 1-7 days

$350.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with dog, human and rat
  • Isotype: IgG1
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Dog, Human, Rat
No reactivity Bovine
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AP125
Immunogen Synthetic peptide (aa 5-27 from N-terminus of human adipophilin/PLIN2)
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Synonym PLIN2, ADRP
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10-1:100 (0.5-5 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (0.5-5 µg/ml; microwave treatment recommended)
Western Blot (WB) 1:50-1:100 (0.5-1 µg/ml)
Background

Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir.
Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets.
Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72.
Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative.Also positively stained are lipid-storing CD 68-positive macrophages.
Tested cultured cell lines: PLC, MDCK.

Learn more about PROGEN Perilipin antibodies.

References/Publications (39)
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Category Protocol
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Category Protocol
Size 919.99 KB
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
  • The fixation method used for lipidic material influences tremendously the quality of staining results.
    Tissue fixation should not exceed 2% paraformaldehyde.
  • Heat induced antigen retrieval is recommended. In many cases a standard protocol using 10 mM citrate buffer (pH 6) works fine.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
  • The use of milk preparations (skim milk, dry milk) for blocking and for antibody dilution buffer might be problematic. The antigen of the Perilipin 2 antibodies was originally found and described in bovine milk and some dry milk brands (or even different lots) might contain the epitopes and block it, thus abolishing the reaction with the antigen transferred onto the Western blot membrane. In this case Tween 20 or BSA or ovalbumin is recommended as e.g. blocking material.
  • Immune reaction with cultured cells: this depends on the age of the cell culture; old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2.
  • Tissue with a high contents of fat might be problematic in electrophoretical separation. One has to load much material onto the SDS gel in order to get enough protein for a positive immunological signal. In addition the protein solubility is sometimes hindered by high fat concentration in the sample.

Positive signals from e.g. cultured cells depend on the age of the cell culture: old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2. With old /aged cells, however, there is always a problem with potential degradation processes.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-Perilipin 2 (C-terminus) guinea pig polyclonal, serum
Cat. No: GP41
  • Guinea pig polyclonal
  • Suitable for IHC, ICC/IF and WB
  • Reacts with human

$356.00*
anti-Perilipin 2 (N-terminus aa 1-29) guinea pig polyclonal, serum
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  • Stabilized antiserum
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  • Suitable for ICC/IF, IHC and WB
  • Reacts with human

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anti-Perilipin 2 (N-terminus aa 6-27) guinea pig polyclonal, serum
Cat. No: GP47
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

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anti-Perilipin 2 (N-terminus aa 1-16) guinea pig polyclonal, serum
Cat. No: GP46
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

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anti-Perilipin 2 (mouse N-terminus) guinea pig polyclonal, serum
Cat. No: GP42
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with mouse

$356.00*