anti-Perilipin 2 (mouse N-terminus) guinea pig polyclonal, serum

Cat. No: GP42

Available, delivery time 1-7 days

$356.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (N-terminal aa 1-16 of murine adipophilin, also named PLIN2): MAAAVVDPQQSVVMRV-C
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID P43883 (Mouse)
Synonym Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:50-1:100
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
Western Blot (WB) 1:500-1:3,000
Background

Adipophilin / ADRP / PLIN2 (a member of the PLIN/PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.1998, 2013; Chong et al.).
Polypeptide reacting: Specific for Adipophilin / ADRP / PLIN2, MW 46,646 (calculated from aa sequence data); apparent Mr 50,000 (after SDS-PAGE); pI 6.42.
Tissue immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes; adipocytes are negative.

Reactivity on cultured cell lines: 3T3-L1, OP9, HL-1

Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

Chong, B. M. et al. The adipophilin C terminus is a self-folding membrane-binding domain that is important for milk lipid secretion. J. Biol. Chem. 286, 23254-23265 (2011).

Heid, H. W., Moll, R., Schwetlick, I., Rackwitz, H. R. & Keenan, T. W. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res. 294, 309-21 (1998).

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FAQs
  • PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
  • Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
  • Use the antibody in a higher dilution, but prolong incubation time and exposure time.
  • Always use a fresh aliquot of the antibody.
  • Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
  • Include an additional washing step.
    You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
  • Always use a fresh aliquot of secondary antibody.
  • In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

  • The fixation method used for lipidic material influences tremendously the quality of staining results.
    Tissue fixation should not exceed 2% paraformaldehyde.
  • Heat induced antigen retrieval is recommended. In many cases a standard protocol using 10 mM citrate buffer (pH 6) works fine.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
  • The use of milk preparations (skim milk, dry milk) for blocking and for antibody dilution buffer might be problematic. The antigen of the Perilipin 2 antibodies was originally found and described in bovine milk and some dry milk brands (or even different lots) might contain the epitopes and block it, thus abolishing the reaction with the antigen transferred onto the Western blot membrane. In this case Tween 20 or BSA or ovalbumin is recommended as e.g. blocking material.
  • Immune reaction with cultured cells: this depends on the age of the cell culture; old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2.
  • Tissue with a high contents of fat might be problematic in electrophoretical separation. One has to load much material onto the SDS gel in order to get enough protein for a positive immunological signal. In addition the protein solubility is sometimes hindered by high fat concentration in the sample.

Positive signals from e.g. cultured cells depend on the age of the cell culture: old cells (more than confluent) tend to accumulate fat and are therefore positive for Perilipin 2. Freshly split cells contain only few lipid droplets and are not easily detectable as positive for Perilipin 2. With old /aged cells, however, there is always a problem with potential degradation processes.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
The concentration of specific antibody in our guinea pig serum is not determined.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.
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  • Goat polyclonal
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Quantity: 50 µg
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  • Isotype: IgG1

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