IHC analysis of human skelatel muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human squamous cell carcinoma using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human skelatel muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 4x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
Griffin, J. D., Bejarano, E., et al. Integrated Action of Autophagy and Adipose Tissue Triglyceride Lipase Ameliorates Diet-Induced Hepatic Steatosis in Liver-Specific PLIN2 Knockout Mice. Cells. 2021-04-25.
Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
IHC analysis of human skelatel muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human squamous cell carcinoma using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
IHC analysis of human skelatel muscle using anti-Perilipin 5 antibody (Cat. No. GP44). IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Perilipin 5 (Cat. No. GP44) was diluted in PBS (1:200) and incubated at 4°C over-night. The secondary antibody biotin anti-guinea pig was incubated for 30 min at RT. Sections were incubated with ABC solution (VectorLaboratories) for 30 min at RT. Slides were stained with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 4x picture was acquired using microscopy (courtesy of J.Hess, University Hospital Heidelberg).
Griffin, J. D., Bejarano, E., et al. Integrated Action of Autophagy and Adipose Tissue Triglyceride Lipase Ameliorates Diet-Induced Hepatic Steatosis in Liver-Specific PLIN2 Knockout Mice. Cells. 2021-04-25.
Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Species/Reactant: Mus musculus (House mouse)
Applications: Western Blotting
Image collected and cropped by CiteAb from the following publication, provided under a CC-BY licence.
Key Features
- Guinea pig polyclonal
- Suitable for ICC/IF and IHC
- Reacts with human
Product description
| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC |
| Purification | Stabilized antiserum |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Synthetic peptides (N-terminal aa 1-17/16-36 of human MLDP) |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | Q00G26 (Human) |
| Synonym | Perilipin-5, Lipid storage droplet protein 5, PLIN5, LSDP5, OXPAT, PAT-1 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:100-1:200 (microwave treatment recommended) |
Background
Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1 and perilipin 5 or PLIN5), MW 50,844 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE).
MLDP / PLIN5 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT.
Additional PLIN/PAT proteins include adipophilin (ADRP, PLIN2), perilipin (PLIN1), and TIP47 (PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
Tissue localization: MLDP/PLIN5 is positively detected in heart and skeletal muscle.
Reactivity on cultured cell lines: HaCat (human), SV80 (human).
References/Publications (3)
Publication
Species
Application
Species
Mouse
Application
WB
Species
mouse
Application
WB
Species
human
Application
WB,ICC-IF
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
- Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
- Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).
- Air-drying of the section.
- Block with the serum of the species in which the secondary antibody was raised for 30 min.
- Incubation with 1st antibody 1 h at RT in moist chamber.
- Wash 3x with PBS.
- Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
- Wash 3x with PBS.
- Immerse shortly into ethanol.
- Let air dry.
- Cover with mounting medium.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
The concentration of specific antibody in our guinea pig serum is not determined.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.
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