anti-Plakophilin 2 guinea pig polyclonal, serum

Cat. No: GP-PP2

Available, delivery time 1-7 days

$357.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse, pig and rat
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Bovine, Human, Mouse, Pig, Rat
No reactivity Amphibia, Fish
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Human synthetic plakophilin 2 (C-terminal aa 820-8 37)
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID A0A3Q1M2G3 (Bovine), Q99959 (Human), Q9CQ73 (Mouse), A0A4X1VWB0 (Pig), H0VL79 (Guinea pig), Q562C0 (Rat)
Synonym Plakophilin-2, PKP2
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100 (preincubated for 5 min with 0.2% Triton X-100 prior to antibody application recommended; longer incubation could result in a loss of nuclear PP2-staining; fixation with 2% formaldehyde to prevent wash-out of soluble forms)
Immunohistochemistry (IHC) - paraffin 1:100 (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

PP2 antiserum stains specifically desmosomal plaques present in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and desmosome-possessing nonepithelial cells such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm.
Polypeptide reacting: plakophilin 2, Mr ~100 kDa (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38).

Reactivity on cultured cell lines: MCF-7, PLC.

References/Publications (3)
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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anti-Plakophilin 3 mouse monoclonal, 310.9.1, supernatant
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  • Reacts with bovine, human and mouse

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  • Mouse monoclonal
  • Suitable for IEM, IHC, IP and WB
  • Reacts with bovine, human and rat
  • Isotype: IgG1

$327.00*