Frequently used

anti-Plakophilin 2 mouse monoclonal, PP2/62 + PP2/86 + PP2/150, supernatant, sample (ready-to-use)

Supernatant

Sample

Cat. No: 651101S

Available, delivery time 1-7 days

$65.00*

Key Features
  • Hybridoma cell culture supernatant, prediluted ready-to-use for IHC
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human and rat
  • Isotype IgG1
Product description
Quantity 600 µl (ready-to-use)
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone PP2/62 + PP2/86 + PP2/150
Immunogen Synthetic carboxyterminal peptide (24 aa) of human plakophilin 2
Formulation Hybridoma cell culture supernatant diluted with PBS pH 7.4 containing 0.5% BSA and 0.09% sodium azide
UniprotID A0A3Q1M2G3 (Bovine), Q99959 (Human), Q562C0 (Rat)
Synonym Plakophilin-2, PKP2
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use (incubate for 5 min in PBS plus 0.2% Triton-X100 prior to antibody application; longer incubation could result in a loss of nuclear staining; fixation with 2% formaldehyde is recommended; exclusive nuckear staining without detergent)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) Assay dependent
Background

The mabs react with plakophilin 2, a member of the arm-repeat family of proteins which can occur in at least 2 splice forms (2a and 2b). Plakophilin 2 assembles with other proteins to form the desmosomal plaque in simple and glandular epithelia, the basal layer of certain stratified epithelia, all layers of some stratified epithelia, and in nonepithelial desmosome-possessing tissues such as myocardium, Purkinje fibers, and dendritic reticulum of lymph node follicles. In all these cells, including a variety of cell types devoid of desmosomes, plakophilin 2 is highly enriched in the karyoplasm. Polypeptide reacting: Plakophilin 2, Mr 100 kDa (SDS-PAGE); MW calculated from aa sequence: 92,750 (pI 9.33) and 97,410 (pI 9.38).

Tumors specifically detected: positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (oesophagus carcinoma). The antibody was negative with all leiomyosarcomas tested.

References/Publications (19)
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Category Protocol
Size 924.86 KB
Filetype pdf
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