Disease related

anti-Plakophilin 3 mouse monoclonal, PKP3-270.6.2, supernatant

Supernatant

Sample

Cat. No: 651113

Available, delivery time 1-7 days

$245.00*

Key Features
  • Hybridoma cell culture supernatant
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human, mouse and rat
  • Isotype IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Mouse, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone PKP3-270.6.2
Immunogen Recombinant protein (E. coli) of human plakophilin 3
Formulation Contains 0.09% sodium azide
UniprotID Q08DQ0 (Bovine), Q9Y446 (Human), Q9QY23 (Mouse), D3ZJ50 (Rat)
Synonym Plakophilin-3, PKP3
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen Undiluted-1:10
Immunohistochemistry (IHC) - paraffin Undiluted-1:10 (microwave treatment recommended)
Western Blot (WB) 1:500
Background

Plakophilin 3 is seen in most desmosomes and partly dispersed in cytoplasm under various fixation conditions (FA, MeAc long, MeAc short):

Positive tissues and culture cells:

  • Stratified squamous epithelia: palmar, epidermis, scalp skin
  • Stratified epithelia: tongue, vagina, esophagus
  • Transitional epithelia: bladder
  • Non-stratified epithelia: colon, duodenum, pancreas, several glands
  • Non-epithelial: reticulum cells of lymph node follicle
  • HaCaT, MCF7, HeLa, HT-29, CaCo 2, Caski, BMGE, MDCK and other

Negative tissues and cell lines:

  • Liver, heart, skeletal muscle, endothelia (vessels)
  • PLC, RD, SV80, HL60, Raji, Jok1, Glioma, K562, 3T3L1, A6, PtK2
References/Publications (4)
Downloads
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Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
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