anti-Prostate-Specific Antigen mouse monoclonal, ER-PR8, ascites fluid
- Mouse monoclonal
- Suitable for ICC/IF, IHC on frozen and paraffin sections and WB
- Reacts with human
- Isotype: IgG1
|Application||ICC/IF, IHC, WB|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated from human prostatic carcinoma|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Prostate-specific antigen, PSA, EC 22.214.171.124, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:10|
|Immunohistochemistry (IHC) - paraffin||1:10 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
The presence of prostate specific antigen is highly specific for demonstration of the prostatic origin of a malignancy. Mab ER-PR8 has been shown to be a valuable reagent for the identification of primary and metastatic prostatic carcinoma. The antibody reacts with a 34 kDa protein in benign and malignant prostatic epithelium.
Positive control: prostatic benign hyperplasia.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.