Epitope mapped
anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate
anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate
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anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate
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Key Features
- Mouse monoclonal
- Suitable for ICC/IF, IHC and WB
- Reacts with drosophila and human
- Isotype: IgG1
Product description
| Quantity | 100 µl |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Hybridoma cell culture supernatant concentrate |
| Reactivity | Drosophila, Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | ARNA-3 |
| Immunogen | Purified drosophila melanogaster RNA polymerase II |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | P24928 (Human) |
| Synonym | DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, EC 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, EC 2.7.7.48, POLR2A, POLR2 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min) |
| Immunohistochemistry (IHC) - frozen | 1:100 |
| Western Blot (WB) | Assay dependent |
Background
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822
References/Publications (5)
Publication
Species
Application
Species
human
Application
WB
Species
human
Application
WB
Species
drosophila
Application
WB
Application
epitope mapping
Species
drosophila
Application
IF
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
- Use wet transfer instead of semidry method.
- Reduce the amount of methanol in the transfer buffer (< 20%).
- Increase the transfer time (e.g. on at 100-200 mA).
- If the protein is low abundant (e.g. nephrin expressed only in podocytes) enrich your protein of interest.
- Pretreatment of the sample with DNase.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.