Epitope mapped
Disease related

anti-RNA Polymerase II mouse monoclonal, ARNA-3, supernatant concentrate

Cat. No: 65223

Available, delivery time 1-7 days

$258.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with drosophila and human
  • Isotype: IgG1
Product description
Quantity 100 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant concentrate
Reactivity Drosophila, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone ARNA-3
Immunogen Purified drosophila melanogaster RNA polymerase II
Formulation Contains 0.09% sodium azide
UniprotID P24928 (Human)
Synonym DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, EC 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, EC 2.7.7.48, POLR2A, POLR2
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min)
Immunohistochemistry (IHC) - frozen 1:100
Western Blot (WB) Assay dependent
Background

ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA.
Epitope recognized within: 794-DDYGPESRGFVENSYLA-822

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