Diagnostic marker

anti-Ribonucleoproteins mouse monoclonal, 58-15, purified

Cat. No: 691520

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for FACS, ICC/IF and IHC
  • Reacts with human and rat
  • Isotype: IgM kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgM kappa
Conjugate Unconjugated
Application FACS, ICC/IF, IHC
Purification Affinity chromatography
Reactivity Human, Rat
No reactivity Mouse
Storage 2-8°C
Intended use Research use only
Clone 58-15
Immunogen Isolated nuclei
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P22626 (Human), A7VJC2 (Rat)
Synonym Heterogeneous nuclear ribonucleoproteins A2/B1, hnRNP A2/B1, HNRNPA2B1, HNRPA2B1
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100-1:200 (0.5-1.0 µg/ml)
Immunohistochemistry (IHC) - frozen 1:25-1:50 (2-4 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:25-1:50 (2-4 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/ml PBS recommended)
Flow Cytometry (FACS) 1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min at 4°C, permeabilize with 0.2% saponin or digitonin for 15 min at 4°C
Background

58-15 recognizes riboncleoproteins (RNP), found predominantly in nuclear ribonucleoprotein (nRNP) particles, one of the main components of nucleoli. It identifies cells active in the cell cycle and, hence, can be used to measure the mitotic activity of cell populations. Since the antibody can be used in paraffin embedded tissue sections, it can identify actively cycling cells within routinely fixed tissue specimens. 58-15 can be considered a pan nRNP antibody. Pan nRNP antibodies provide detection for a range of RNP proteins.

Positive control: Jurkat cells, K-562 cells, spleen.

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