anti-Serotonin 5-HT rabbit polyclonal, serum
Species/Reactant: Mus musculus (House mouse)
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- Rabbit polyclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with human, mouse and rat
|Quantity||100 µl (lyoph.)|
|Reactivity||Human, Mouse, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Serotonin conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|UniprotID||P08908 (Human),Q64264 (Mouse),P19327 (Rat)|
|Synonym||5-hydroxytryptamine receptor 1A, 5-HT-1A, 5-HT1A, G-21, Serotonin receptor 1A, HTR1A, ADRB2RL1, ADRBRL1|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:200-1:400|
|Immunohistochemistry (IHC) - frozen||1:200-1:400|
|Immunohistochemistry (IHC) - paraffin||1:200-1:400 (microwave treatment recommended)|
Serotonin is produced by endocrine cells of the stomach, duodenum and ileum. The polyclonal antibody to serotonin can be used to differentiate tumors of serotoninergic origin. The antigen localization is cytoplasmic.
Absorption with 10-100 ug serotonin per ml diluted antiserum abolishes the staining.
Positive control: duodenum.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.