Disease related

anti-Symplekin mouse monoclonal, Sym-TJ-E150, supernatant

Cat. No: 651100

Available, delivery time 1-7 days

$245.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Rat
No reactivity Bovine, Mouse, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Sym-TJ-E150
Immunogen Human symplekin
Formulation Contains 0.09% sodium azide
UniprotID Q92797 (Human), F1LSH0 (Rat)
Synonym Symplekin, SYMPK, SPK
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use (for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended; for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Western Blot (WB) Assay dependent
Background

The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; non-epithelia such as fibroblasts, endothelia, lymphocytes).

Polypeptide reacting: symplekin, 126.5 kDa polypeptide (calculated from aa sequence data); apparent Mr 150 kDa (after SDS-PAGE); pI 6.5.

Reactivities on cultured cell lines: carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG/343 MG (glioma), K 562 (erythroleukemia), RVF (rat vascular smooth muscle cells).

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.