Disease related

anti-Symplekin mouse monoclonal, Sym-TJ-E150, supernatant

Cat. No: 651100

Available, delivery time 1-7 days

$245.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with human and rat
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Rat
No reactivity Bovine, Mouse, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone Sym-TJ-E150
Immunogen Human symplekin
Formulation Contains 0.09% sodium azide
UniprotID Q92797 (Human), F1LSH0 (Rat)
Synonym Symplekin, SYMPK, SPK
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen Ready-to-use (for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended; for nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash)
Western Blot (WB) Assay dependent
Background

The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; non-epithelia such as fibroblasts, endothelia, lymphocytes).

Polypeptide reacting: symplekin, 126.5 kDa polypeptide (calculated from aa sequence data); apparent Mr 150 kDa (after SDS-PAGE); pI 6.5.

Reactivities on cultured cell lines: carcinoma cell lines (e.g. MCF-7, Caco-2, HT29, PLC), SV80 (fibroblasts), U333 CG/343 MG (glioma), K 562 (erythroleukemia), RVF (rat vascular smooth muscle cells).

References/Publications (1)
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