anti-Synaptopodin/SYNPO (internal N-terminus) guinea pig polyclonal, serum

Cat. No: GP94-INN

Available, delivery time 1-7 days

$374.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Stabilized antiserum
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptides (mouse internal N-terminal sequence), coupled to KLH
Formulation Contains 0.09% sodium azide and 0.5% BSA
UniprotID Q8N3V7 (Human),Q91YE8 (Mouse)
Synonym Synaptopodin, SYNPO, KIAA1029
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:50
Immunohistochemistry (IHC) - paraffin 1:50 (microwave treatment recommended)
Western Blot (WB) 1:500
Background

The antibody reacts specifically with an epitope in the internal part of synaptopodin/SYNPO, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin.
Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons.

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