Disease related

anti-TGF-alpha mouse monoclonal, 1E8-G6, purified

Cat. No: 691508

Available, delivery time 1-7 days

$425.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, FACS, IHC and WB
  • Reacts with human, rabbit and zebrafish
  • Isotype: IgM kappa
Product description
Quantity 1 ml (100 µg/ml)
Antibody Type Monoclonal
Host Mouse
Isotype IgM kappa
Conjugate Unconjugated
Application ELISA, FACS, IHC, WB
Purification Affinity chromatography
Reactivity Human, Rabbit, Zebrafish
Storage 2-8°C
Intended use Research use only
Clone 1E8-G6
Immunogen Synthetic TGF-alpha 34-43 decapeptide linked carrier
Concentration 100 µg/ml
Formulation PBS with 0.02% sodium azide
UniprotID P01135 (Human), P98138 (Rabbit), Q7T011 (Zebrafish, Brachydanio rerio)
Synonym Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1], TGFA
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:50-1:100 (1-2 µg/ml)
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (1-2 µg/ml; microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
ELISA Assay dependent
Flow Cytometry (FACS) 0.5-1.0 µg/million cells in 0.1 ml
Western Blot (WB) 1:50-1:100 (1-2 µg/ml)
Background

1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained.

Positive control: Jurkat or Ramos cells, testicular carcinoma, breast cancer.

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