Disease related

anti-Uroplakin III mouse monoclonal, AU1, liquid, purified, small

Liquid

Lyophilized

Liquid

Cat. No: 690108S

Available, delivery time 1-7 days

$170.00*

Key Features
  • Purified, liquid, small
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, pig and rat
  • Isotype: IgG1
Product description
Quantity 200 µl
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Pig, Rat
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AU1
Immunogen Asymmetric unit membrane (AUM) preparation from bovine urinary bladder
Concentration 100 µg/ml
Formulation 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
UniprotID Q864V4 (Bovine), Q9BT76 (Human), A0A4X1ULK0 (Pig), H0VUG7 (Guinea pig), D3ZKW6 (Rat)
Synonym Uroplakin-3b, UP3b, Uroplakin IIIb, UPIIIb, p35, UPK3B
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - paraffin 1:20-1:60 (microwave treatment recommended)
Western Blot (WB) 1:4,000-1:20,000
Background

Mab AU1 reacts specifically with uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kDa) and has been shown to be a specific marker of terminal urothelial differentia-tion (Wu et al. 1993 & 1194).
Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra.
About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995).
Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Moll, R., Wu, X.-R., Lin, J.-H. & Sunt, T.-T. Uroplakins, Specific Membrane Proteins of Urothelial Umbrella Cells, as Histological Markers of Metastatic Transitional Cell Carcinomas. Am. J. Pathol. 147, 1383-1397 (1995).
Wu, X.-R. et al. Mammalian Uroplakins: A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269, 13716-13724 (1994).
Wu, X. R. & Sun, T. T. Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106, 31-43 (1993).

References/Publications (3)
Downloads
File
Category
Size
Filetype
Category Protocol
Size 924.86 KB
Filetype pdf
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.