Disease related

anti-Uroplakin III mouse monoclonal, AU1, lyophilized, purified

Liquid

Lyophilized

Liquid

Cat. No: 610108

Available, delivery time 1-7 days

$373.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, pig and rat
  • Isotype: IgG1
Product description
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Human, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone AU1
Immunogen Asymmetric unit membrane (AUM) preparation from bovine urinary bladder
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID O88457 (Rat)
Synonym Uroplakin-3b, UP3b, Uroplakin IIIb, UPIIIb, p35, UPK3B
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - paraffin 1:10-1:30 (microwave treatment recommended)
Western Blot (WB) 1:2,000-1:10,000
Background

Mab AU1 reacts specifically with uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kDa) and has been shown to be a specific marker of terminal urothelial differentia-tion (Wu et al. 1993 & 1194).
Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra.
About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995).
Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Moll, R., Wu, X.-R., Lin, J.-H. & Sunt, T.-T. Uroplakins, Specific Membrane Proteins of Urothelial Umbrella Cells, as Histological Markers of Metastatic Transitional Cell Carcinomas. Am. J. Pathol. 147, 1383-1397 (1995).
Wu, X.-R. et al. Mammalian Uroplakins: A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269, 13716-13724 (1994).
Wu, X. R. & Sun, T. T. Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106, 31-43 (1993).

References/Publications (3)
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