anti-Vesicular Monoamine Transporter VMAT2 guinea pig polyclonal, serum
- Guinea pig polyclonal
- Suitable for IHC on frozen sections and WB
- Reacts with human, mouse, pig and rat
|Quantity||50 µl (lyoph.)|
|Reactivity||Human, Mouse, Pig, Rat|
|Storage before reconstitution||2-8°C until indicated expiry date|
|Storage after reconstitution||Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide (CQSYPIGDDEESESD-OH) from the C-terminus of rat VMAT2 conjugated to BSA|
|Formulation||Lyophilized; reconstitute in 100 µl dist. water|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:2,000-1:3,000|
|Western Blot (WB)||Assay dependent|
The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. The antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining. In Western blot experiments using a crude synaptic vesicle fraction of rat brain, the 70 kDa of anti-VMaT2 is recognized (suggested dilution 1:500).
Positive control: frozen sections of rat small intestine.
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- Rabbit polyclonal
- Suitable for IHC on frozen sections
- Reacts with dog, guinea pig, hamster, human, mouse and rat