anti-Vesicular Monoamine Transporter VMAT2 guinea pig polyclonal, serum

Cat. No: 16085

Available, delivery time 1-7 days

$514.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for IHC on frozen sections and WB
  • Reacts with human, mouse, pig and rat
Product description
Quantity 50 µl (lyoph.)
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application IHC, WB
Purification Undiluted antiserum
Reactivity Human, Mouse, Pig, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Synthetic peptide (CQSYPIGDDEESESD-OH) from the C-terminus of rat VMAT2 conjugated to BSA
Formulation Lyophilized; reconstitute in 100 µl dist. water
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:2,000-1:3,000
Western Blot (WB) Assay dependent
Background

The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. The antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining. In Western blot experiments using a crude synaptic vesicle fraction of rat brain, the 70 kDa of anti-VMaT2 is recognized (suggested dilution 1:500).

Positive control: frozen sections of rat small intestine.

References/Publications (1)
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