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anti-Vimentin, VIM 3B4 + positive western blot control

anti-Vimentin, VIM 3B4 + positive western blot control
WB with anti-Vimentin antibody (Cat. No. 61013, 1:500), SV80 whole cell lysate (Cat. No. 64001, 10 µg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on human skin (courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on mouse squamous-cell carcinoma (SCC)(courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on human skin (courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on mouse tongue (courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
IHC-P with anti-Vimentin antibody on human oesophagus (courtesy of J.Heß, University Hospital Heidelberg) View more
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
anti-Vimentin, VIM 3B4 + positive western blot control
Cat. No: 61013-WBC

Prices excl. VAT and shipping costs

Available, delivery time 1-7 days

$314.00*

Datasheet
Key Features
The set provides anti-Vimentin, VIM 3B4 antibody and associated Western blot control - positively tested SV80 cell lysate.
The set contains enough Western blot control to perform 5 Western blots.
The anti-Vimentin, VIM 3B4 antibody is an excellent marker for mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma). The antibody is highly specific for the intermediate filament protein vimentin present in all cells of mesenchymal origin.

Included products:

Product description
Quantity 50 µg anti-Vimentin + 50 µg western blot control
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ELISA, ICC, IHC, WB
Purification Affinity chromatography, Cell culture lysate
Reactivity Amphibia, Bovine, Chicken, Human, Monkey
Storage Antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/thaw cycles for both components
Intended use Research use only
Clone VIM 3B4
Immunogen Vimentin purified from bovine lens
Formulation Antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µl 1x SDS buffer (lysis buffer composition: PBS + Pefablock)
UniprotID P48616 (Bovine),P09654 (Chicken),P08670 (Human),V9HWE1 (Human)
Synonym Vimentin, VIM
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Vimentin: assay dependent
Immunohistochemistry (IHC) - frozen anti-Vimentin: 1:100-1:200
Immunohistochemistry (IHC) - paraffin anti-Vimentin: 1:100-1:200 (microwave treatment recommended)
ELISA anti-Vimentin: assay dependent
Western Blot (WB) anti-Vimentin: 1:500; western blot control: 10 µg total protein per lane
Background

anti-Vimentin antibody:
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Bohn W, Wiegers W, Beuttenmueller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).

Positive western blot control:
Whole Cell Lysate from SV80 Human Lung fibroblast (SV40-transformed) as positive western blot control.
SV80 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. 61013, anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified
Cat. No. 64001, Positive western blot control: anti-Vimentin antibody

References/Publications (22)
Publication
Species
Application
Publication Soglia, F. et al. Distribution and Expression of Vimentin and Desmin in Broiler Pectoralis major Affected by the Growth-Related Muscular Abnormalities. Front.Physiol. 10, 1581 (2020)
Species chicken
Application WB,IHC (frozen),IHC
Publication Aguirre-Portolés, C., et al. ABCA1 overexpression worsens colorectal cancer prognosis by facilitating tumour growth and caveolin-1-dependent invasiveness, and ... Mol. Oncol. 12, 1735–1752 (2018).
Species human
Application ICC-IF
Publication Cossu, G. et al. An exceptional presentation of pituicytoma apoplexy: A case report. Oncol.Lett. 16, 643-647 (2018)
Species human
Application IHC (paraffin)
Publication Zayas-Santiago, A. et al. Unidirectional photoreceptor-to-Müller glia coupling and unique K+ channel expression in Caiman retina. PLoS One 9, (2014).
Species caiman
Application IHC
Publication Heid, H. et al. On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9, e90386 (2014).
Species human
Application WB,ICC-IF
Publication Lever, M., Brand-Saberi, B. & Theiss, C. Neurogenesis, gliogenesis and the developing chicken optic tectum: an immunohistochemical and ultrastructural analysis. Brain Struct. Funct. 219, 1009-1024 (2014).
Species chicken
Application IHC (frozen)
Publication Rickelt, S. et al. A novel kind of tumor type-characteristic junction: plakophilin-2 as a major protein of adherens junctions in cardiac myxomata. Mod. Pathol. 23, 1429-1437 (2010).
Species human
Application WB
Publication Martinez-Iglesias, O., Garcia-Silva, S., Regadera, J. & Aranda, A. Hypothyroidism Enhances Tumor Invasiveness and Metastasis Development. PLoS One 4, (2009).
Species human
Application IHC (paraffin)
Publication Straub, B. K., Stoeffel, P., Heid, H., Zimbelmann, R. & Schirmacher, P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47, 1936-1946 (2008).
Species human
Application IHC (frozen)
Publication Moll, R. et al. The cardiac isoform of ?-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies. Virchows Arch. 449, 175-191 (2006).
Species human
Application IHC (paraffin)
Publication Bär, H. et al. Severe muscle disease-causing desmin mutations interfere with in vitro filament assembly at distinct stages. Proc. Natl. Acad. Sci. U. S. A. 102, 15099-104 (2005).
Species human
Application ICC-IF
Publication Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337-347 (2003).
Species human
Application IHC (frozen)
Publication Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114-30 (2003).
Species human
Application ICC-IF
Publication Riedel, I. et al. Brenner tumors but not transitional cell carcinomas of the ovary show urothelial differentiation: immunohistochemical staining of urothelial markers, including cytokeratins and uroplakins. Virchows Arch. 438, 181-91 (2001).
Species human
Application IHC (paraffin)
Publication Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437-46 (1999).
Species human
Application IHC (frozen)
Publication Gomi, H. et al. Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron 14, 29-41 (1995).
Species human
Application IHC (paraffin)
Publication Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518-35 (1995).
Species human
Application IHC (paraffin)
Publication Herrmann, H., Eckelt, A., Brettel, M., Grund, C. & Franke, W. W. Temperature-sensitive Intermediate Filament Assembly. J. Mol. Biol. 234, 99-113 (1993).
Species human,xenopus
Application ICC-IF
Publication Bohn, W., Wiegers, W., Beuttenmüller, M. & Traub, P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp. Cell Res. 201, 1-7 (1992).
Species human,monkey,rabbit,bovine,chicken,dog,equine
Application WB,ICC-IF
Publication Knapp, A. C. & Franke, W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell 59, 67-79 (1989).
Species human
Application WB,ICC-IF
Publication Rungger-Brandle, E., Achtstätter, T. & Franke, W. W. An epithelium-type cytoskeleton in a glial cell: Astrocytes of amphibian optic nerves contain cytokeratin filaments and are connected by desmosomes. J. Cell Biol. 109, 705-716 (1989).
Species xenopus
Application IHC (frozen)
Publication Herrmann, H., Fouquet, B. & Franke, W. W. Expression of intermediate filament proteins during development of Xenopus laevis. I. cDNA clones encoding different forms of vimentin. Development 105, 279-98 (1989).
Species xenopus
Application WB,IHC (frozen),ICC-IF
Downloads
File
Category
Size
Filetype
File WB protocol
Category Protocol
Size 262.98 KB
Filetype pdf
Datasheet
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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  • Mouse monoclonal
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