Frequently used

anti-Vimentin, VIM 3B4 + positive western blot control

Cat. No: 61013-WBC

Available, delivery time 1-7 days


Key Features
The set provides anti-Vimentin, VIM 3B4 antibody and associated Western blot control - positively tested SV80 cell lysate.
The set contains enough Western blot control to perform 5 Western blots.
The anti-Vimentin, VIM 3B4 antibody is an excellent marker for mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma). The antibody is highly specific for the intermediate filament protein vimentin present in all cells of mesenchymal origin.

Included products:

Product description
Quantity 50 µg anti-Vimentin + 50 µg western blot control
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a kappa
Conjugate Unconjugated
Application ELISA, ICC, IHC, WB
Purification Affinity chromatography, Cell culture lysate
Reactivity Amphibia, Bovine, Chicken, Human, Monkey
Storage Antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/thaw cycles for both components
Intended use Research use only
Clone VIM 3B4
Immunogen Vimentin purified from bovine lens
Formulation Antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µl 1x SDS buffer (lysis buffer composition: PBS + Pefablock)
UniprotID P48616 (Bovine),P09654 (Chicken),P08670 (Human),V9HWE1 (Human)
Synonym Vimentin, VIM
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) anti-Vimentin: assay dependent
Immunohistochemistry (IHC) - frozen anti-Vimentin: 1:100-1:200
Immunohistochemistry (IHC) - paraffin anti-Vimentin: 1:100-1:200 (microwave treatment recommended)
ELISA anti-Vimentin: assay dependent
Western Blot (WB) anti-Vimentin: 1:500; western blot control: 10 µg total protein per lane

anti-Vimentin antibody:
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).

Bohn W, Wiegers W, Beuttenmueller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).

Positive western blot control:
Whole Cell Lysate from SV80 Human Lung fibroblast (SV40-transformed) as positive western blot control.
SV80 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.

Set content:
Cat. No. 61013, anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified
Cat. No. 64001, Positive western blot control: anti-Vimentin antibody

References/Publications (21)
Category Protocol
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Filetype pdf
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Positive western blot control: anti-Vimentin antibody
Cat. No: 64001
  • Whole cell lysate from SV80 human lung fibroblast (SV40-transformed) cell line
  • Validated and tested western blot control for: anti-Vimentin mouse monoclonal antibody, VIM 3B4, Cat. No. 61013

anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified
Cat. No: 61013
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, chicken, human and monkey
  • Isotype: IgG2a

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