anti-Vimentin, VIM 3B4 + positive western blot control
The set contains enough Western blot control to perform 5 Western blots.
The anti-Vimentin, VIM 3B4 antibody is an excellent marker for mesenchymal cells and mesenchyme-derived tumors (sarcoma, lymphoma, melanoma). The antibody is highly specific for the intermediate filament protein vimentin present in all cells of mesenchymal origin.
Included products:
Product description
Quantity | 50 µg anti-Vimentin + 50 µg western blot control |
---|---|
Antibody Type | Monoclonal |
Host | Mouse |
Isotype | IgG2a kappa |
Conjugate | Unconjugated |
Application | ELISA, ICC, IHC, WB |
Purification | Affinity chromatography, Cell culture lysate |
Reactivity | Amphibia, Bovine, Chicken, Human, Monkey |
Storage | Antibody: short term at 2-8°C; long term in aliquots at -20°C; western blot control: lyophilized at 2-8°C, reconstituted at -20°C; avoid freeze/thaw cycles for both components |
Intended use | Research use only |
Clone | VIM 3B4 |
Immunogen | Vimentin purified from bovine lens |
Formulation | Antibody: lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4); western blot control: lyophilized, reconstitute in 50 µl 1x SDS buffer (lysis buffer composition: PBS + Pefablock) |
UniprotID | P48616 (Bovine),P09654 (Chicken),P08670 (Human),V9HWE1 (Human) |
Synonym | Vimentin, VIM |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | anti-Vimentin: assay dependent |
Immunohistochemistry (IHC) - frozen | anti-Vimentin: 1:100-1:200 |
Immunohistochemistry (IHC) - paraffin | anti-Vimentin: 1:100-1:200 (microwave treatment recommended) |
ELISA | anti-Vimentin: assay dependent |
Western Blot (WB) | anti-Vimentin: 1:500; western blot control: 10 µg total protein per lane |
Background
anti-Vimentin antibody:
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin.
Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells.
Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.
The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Bohn W, Wiegers W, Beuttenmueller M, Traub P: Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res 201: 1-7 (1992).
Rogers KR, Eckelt A, Nimmrich V, Janssen K-P, Schliwa M, Herrmann H, Franke WW: Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly. Eur J Cell Biol 66: 136-150 (1995).
Positive western blot control:
Whole Cell Lysate from SV80 Human Lung fibroblast (SV40-transformed) as positive western blot control.
SV80 whole cell lysate was prepared by homogenization in PBS containing Pefablock.
Protein concentration was determined using Bradford assay.
Set content:
Cat. No. 61013, anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified
Cat. No. 64001, Positive western blot control: anti-Vimentin antibody
References/Publications (22)
Downloads
Q & A's
Customer Reviews
Login
FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.