anti-Vimentin guinea pig polyclonal, lyophilized, purified

Cat. No: 610130

Available, delivery time 1-7 days

$335.00*

Key Features
  • Guinea pig polyclonal
  • Suitable for ELISA, IHC and WB
  • Reacts with human and mouse
Product description
Quantity 50 µg
Antibody Type Polyclonal
Host Guinea pig
Conjugate Unconjugated
Application ELISA, IHC, WB
Purification Affinity chromatography
Reactivity Human, Mouse
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Immunogen Human Vimentin
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID P08670 (Human), P20152 (Mouse)
Synonym Vimentin, VIM
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - paraffin 1:10-1:100 (0.5-5 µg/ml, microwave treatment recommended)
ELISA Assay dependent
Western Blot (WB) 1:3,000 (0.02 µg/ml)
Background
Specific detection of vimentin (57 kDa polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Downloads
Q & A's

Please enter a valid email address.
Please enter a valid question.
Fields marked with * are mandatory.

There aren't any asked questions yet.
Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.


FAQs
  • PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
  • Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
  • Use the antibody in a higher dilution, but prolong incubation time and exposure time.
  • Always use a fresh aliquot of the antibody.
  • Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
  • Include an additional washing step.
    You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
  • Always use a fresh aliquot of secondary antibody.
  • In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Please find the solvent for reconstitution and the amount of liquid for reconstitution on the antibody datasheet. The buffer formulation after reconstitution is also mentioned in the datasheet.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-guinea pig IgG goat polyclonal, HRP conjugate
Cat. No: 90001
  • Goat polyclonal
  • Suitable for ELISA and WB
  • Reacts with guinea pig

$109.00*
anti-Vimentin mouse monoclonal, XL-VIM-14.13, supernatant
Cat. No: 65189
  • Mouse monoclonal
  • Suitable for IEM, IHC and WB
  • Reacts with huamn, carp and trout
  • Isotype: IgG1

$245.00*
anti-Vimentin mouse monoclonal, VIM 3B4, lyophilized, purified
Cat. No: 61013
Quantity: 50 µg
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF, IHC and WB
  • Reacts with amphibia, bovine, chicken, human, mouse and monkey
  • Isotype: IgG2a

Variants from $96.00*
$304.00*
anti-Vimentin guinea pig polyclonal, serum
Cat. No: GP53
  • Guinea pig poplyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, human and mouse

$306.00*
anti-Vimentin (N-terminus) guinea pig polyclonal, serum
Cat. No: GP59
  • Guinea pig poplyclonal
  • Suitable for IHC and WB
  • Reacts with human and mouse

$332.00*
anti-Vimentin (C-terminus) guinea pig polyclonal, serum
Cat. No: GP58
  • Guinea pig poplyclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with human and mouse

$332.00*
Vimentin, bovine, 250 µg
Cat. No: 62011
Quantity: 250 µg
  • Purified bovine vimentin
  • Protein standard

Variants from $364.00*
$673.00*
Vimentin, human recombinant, 250 µg
Cat. No: 62015
Quantity: 250 µg
  • Recombinant human vimentin
  • Protein standard

Variants from $364.00*
$746.00*