Key Features
- Mouse monoclonal
- Suitable for Agglutination, ICC/IF and IHC
- Reacts with Y. enterocolitica O:3
- Isotype: IgG3
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG3 |
| Conjugate | Unconjugated |
| Application | Agglutination, ICC/IF, IHC |
| Purification | Affinity chromatography |
| Reactivity | Y. enterocolitica O:3 |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | 2D8 |
| Immunogen | Yersinia enterocolitica serogroup O:3 strain |
| Concentration | 100 µg/ml |
| Formulation | PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:20 |
| Immunohistochemistry (IHC) - frozen | 1:20 |
| Immunohistochemistry (IHC) - paraffin | 1:20 (microwave treatment recommended) |
| Agglutination | 1:40 +/- 1 titer unit |
Background
2D8 specifically reacts with Yersinia enterocolitica serogroup O:3 strains in both agglutination and immunofluorescence assays.
The antibody does not react with Y. enterocolitica O:9 and O:8 serogroups.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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Quantity:
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