anti-acidic Hair Keratin K33 guinea pig polyclonal, serum
- Guinea pig polyclonal
- Suitable for IHC and WB
- Reacts with human and mouse
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide of human hair (trichocytic) keratin K33 (formerly also designated keratin hHa3; EKP IGS CVT NPC GPR SR), coupled to KLH|
|Formulation||Contains 0.09% sodium azide and 0.5% BSA|
|UniprotID||O76009 (Human), Q8K0Y2 (Mouse), Q14525 (Human), Q61897 (Mouse)|
|Synonym||Keratin, type I cuticular Ha3-I, Hair keratin, type I Ha3-I, Keratin-33A, K33A, KRT33A, HHA3-I, HKA3A, KRTHA3A|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1% Triton X-100 (in PBS) for 1-5 min prior to first antibody incubation step)|
|Western Blot (WB)||1:2,000|
The antiserum stains specifically human hair keratin K33 expressed starting from the middle hair cortex.
Because of high sequence homology both keratin isoforms K33a (formerly also designated keratin hHa3-I) and K33b (formerly designated keratin hHa3-II) are detected.
Q & A's
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.