anti-alpha-6 Integrin rat monoclonal, GoH3, supernatant
- Rat monoclonal
- Suitable for IHC on frozen sections
- Reacts with human
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Mouse mammary tumor cells|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD antigen CD49f [Cleaved into: Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6, Alpha6p], ITGA6|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
In cutaneous melanocytic lesions, the level of expression of three integrin subunits (a2, a6, and beta3) has been found to be correlated with tumor progression. Mab GoH3, directed against alpha 6-integrins/ CD49f (Laminin receptor, VLA-6), shows a cytoplasmic staining pattern of uveal and cutaneous melanoma cells. Antibody blocks binding of integrin a6 to laminin P1 and E8. In WB reactive with two major bands of 120 and 140 kD under non-reducing conditions.
Positive control: Epithelial tissues.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.