anti-alpha-6 Integrin (CD49) rat monoclonal, GoH3, supernatant
anti-alpha-6 Integrin (CD49) rat monoclonal, GoH3, supernatant
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anti-alpha-6 Integrin (CD49) rat monoclonal, GoH3, supernatant
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Key Features
- Rat monoclonal
- Suitable for IHC on frozen sections
- Reacts with human
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Rat |
| Isotype | IgG2a |
| Conjugate | Unconjugated |
| Application | IHC |
| Purification | Hybridoma cell culture supernatant |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | GoH3 |
| Immunogen | Mouse mammary tumor cells |
| Formulation | Contains 0.09% sodium azide |
| Synonym | Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD antigen CD49f [Cleaved into: Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6, Alpha6p], ITGA6 |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:5-1:10 |
Background
In cutaneous melanocytic lesions, the level of expression of three integrin subunits (a2, a6, and beta3) has been found to be correlated with tumor progression. Mab GoH3, directed against alpha 6-integrins/ CD49f (Laminin receptor, VLA-6), shows a cytoplasmic staining pattern of uveal and cutaneous melanoma cells. Antibody blocks binding of integrin a6 to laminin P1 and E8. In WB reactive with two major bands of 120 and 140 kD under non-reducing conditions.
Positive control: Epithelial tissues.
References/Publications (4)
Publication
Species
Application
Publication
Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)
Species
bovine
Application
ICC-IF
Species
human,mouse
Application
IHC (frozen)
Species
human
Application
IHC (frozen)
Species
human,mouse
Application
WB,IHC (frozen),IEM,IP
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.