anti-guinea pig IgG goat polyclonal, HRP conjugate
Key Features
- Goat polyclonal
- Suitable for ELISA and WB
- Reacts with guinea pig
Product description
Quantity | 100 µg |
---|---|
Antibody Type | Polyclonal |
Host | Goat |
Conjugate | Peroxidase/HRP |
Application | ELISA, WB |
Purification | Affinity chromatography |
Reactivity | Guinea pig |
Storage before reconstitution | 2-8°C until indicated expiry date |
Storage after reconstitution | Up to 1 month at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
Intended use | Research use only |
Immunogen | Guinea pig IgG |
Formulation | Lyophilized, reconstitute in 500 µl dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4) |
Applications
Tested applications | Tested dilutions |
---|---|
ELISA | 1:1,250-1:25,000 |
Western Blot (WB) | 1:2,500-1:100,000 |
Background
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.