Disease related

anti-human Ig lambda mouse monoclonal, 48.0, supernatant

Cat. No: 11011

Available, delivery time 1-7 days

$244.00*

Key Features
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1
Product description
Quantity 1 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ELISA, ICC/IF, IHC
Purification Hybridoma cell culture supernatant
Reactivity Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 48
Immunogen Isolated from Bence Jones lambda proteins
Formulation Contains 0.09% sodium azide
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunohistochemistry (IHC) - paraffin 1:5-1:10 (microwave treatment recommended)
ELISA Assay dependent
Background

Suitable for characterization of malignant B-cell proliferations. All but acute lymphocytic leukemias share either surface or intra-cytoplasmic Ig with an isotypic restriction suggesting a monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenstoem's disease bear intracytoplasmic IgM. The other isotypes are found less frequently; multiple myelomas are usually of the IgG or IgA type. Also suitable for the characterization of plasma cells in intestinal inflammatory conditions, e.g. for the classification of intestinal bowel disease and allergic conditions.
In the latter case a specific increase in the number of IgE plasma cells can be demonstrated.
In ELISA the antibody reacts with lambda light chains of secreted immunoglobulins and with bound Ig on the surface of B lymphocytes.
Positive control: tonsil.

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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
anti-human IgE mouse monoclonal, MH25-1, purified
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  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG2b

$244.00*
anti-human IgA mouse monoclonal, MH14-1, purified
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anti-human IgG mouse monoclonal, MH16-1, purified
Cat. No: 11003
  • Mouse monoclonal
  • Suitable for ELISA, ICC/IF and IHC on frozen and paraffin sections
  • Reacts with human
  • Isotype: IgG1 kappa

$244.00*