anti-human IgG mouse monoclonal, MH16-1, purified
- Mouse monoclonal
- Suitable for ELISA, ICC/IF and IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG1 kappa
|Application||ELISA, ICC/IF, IHC|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Isolated human IgG|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (IHC) - frozen||1:5-1:10|
|Immunohistochemistry (IHC) - paraffin||1:5-1:10|
MH16-1 recognizes IgG heavy chains. The epitope is present on the Fc part of IgG. No cross reactivity with IgA or IgM (tested with ELISA).
Suitable for lymphoma phenotyping. Multiple myelomas are of the IgG or IgA type.
Suitable for characterization of plasma cells in inflammatory conditions.
Positive control: tonsil.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.