anti-mTOR rabbit polyclonal, purified, serum

Cat. No: 490947

Available, delivery time 1-7 days

$249.00*

Key Features
  • Rabbit polyclonal
  • Suitable for IHC, ICC/IF, IP and WB
  • Reacts with human, mouse, dog, monkey and rat
  • Knock out validated
Product description
Quantity 100 µl
Antibody Type Polyclonal
Host Rabbit
Conjugate Unconjugated
Application ICC/IF, IHC, IP, WB
Purification Affinity chromatography
Reactivity Dog, Human, Monkey, Mouse, Rat
Storage Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Immunogen Peptide from human mTOR (GTTVPESIHSFIGDGLVKPE)
Concentration 500 µg/ml
Formulation PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA
UniprotID P42345 (Human)
Synonym Mechanistic target of rapamycin, Mammalian target of rapamycin
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:500-1:2,000
Immunohistochemistry (IHC) - paraffin 1:50-1:100 (microwave treatment recommended)
Immunoprecipitation (IP) 10 µg
Western Blot (WB) 1:500-1:2,000
Background

Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in protein synthesis, cell growth, proliferation and differentiation. It is inhibited by rapamycin, which has immunosuppressant functions. The 290 kDa protein is highly regulated by growth factors and nutrients like glucose and amino acids. mTOR is involved in several pathways from protein degradation via autophagy to protein synthesis via translation initiation (4EBP), ribosome and lipid synthesis. It is located in vesicles and the cytosol.


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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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