Epitope mapped
Disease related

anti-nup153 mouse monoclonal, nup7A8, supernatant

Cat. No: 651141

Available, delivery time 1-7 days

$245.00*

Key Features
  • Mouse monoclonal
  • Suitable for IHC, IP and WB
  • Reacts with human, mouse, rat, rat kangaroo and xenopus
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application IHC, IP, WB
Purification Hybridoma cell culture supernatant
Reactivity Human, Mouse, Rat, Rat kangaroo, Xenopus
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone nup7A8
Immunogen Nuclear matrix protein fraction prepared from rat liver nuclei
Formulation Contains 0.09% sodium azide
UniprotID P49790 (Human), Q80WR0 (Mouse), P49791 (Rat)
Synonym Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:5-1:10
Immunoprecipitation (IP) Assay dependent
Western Blot (WB) 1:30-1:50
Background

The antibody recognizes the nuclear pore complex (NPC) protein nup153 (190 kDa), present on the inner aspect of NPC with attached filament bundles. It is also reactive with the soluble form of nup153 found e.g. during meiotic metaphase. Epitope in rat liver was mapped to N-terminal domain (aa439-611; Hase & Cordes, 2003).

Hase ME, Cordes VC: Direct interaction with nup153 mediates binding of tpr to the periphery of the nuclear pore complex. Mol Biol Cell 14, 1923-1940 (2003).

References/Publications (3)
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FAQs
The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

  • Wash cells 1x with PBS (37°C).
  • Fix in 2% freshly made PFA in PBS for 10-15 min.
  • 2x 10 min 50 mM NH4Cl in PBS.
  • 1x 10 min 0.1% Saponin in PBS with NH4Cl or 1x 5 min 0.1-0.5% Triton-X100 in PBS.
  • Optional: antigens difficult to access: incubate with 5 min ice-cold methanol and 30-60 sec with ice-cold acetone.
  • Let air dry.
  • Block with the serum of the species in which the secondary antibody was raised for 30 min at RT.
  • Incubate with primary antibody overnight at 4°C.
  • Wash 3x with PBS at RT.
  • Incubate 1 h with fluorochrome-conjugated secondary antibody.
  • Wash 2x with PBS.
  • Embed in mounting medium.

PROGEN offers different formats for mouse monoclonal antibodies.  Different antibodies may not be offered in each format, the following are all possible formats:
  1. Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
  2. Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet. 
  3. Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet. 
  4. Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page?
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet. 
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.