anti-p0071 mouse monoclonal, 7.7.9, supernatant

Cat. No: 651165

Available, delivery time 1-7 days

$251.00*

Key Features
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine and human
  • Isotype: IgG1
Product description
Quantity 5 ml
Antibody Type Monoclonal
Host Mouse
Isotype IgG1
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Hybridoma cell culture supernatant
Reactivity Bovine, Human
Storage Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 7.7.9
Immunogen Synthetic peptide of human p0071 (aa position 85-104: EKSFPWRSTDVNTGVSKPR)
Formulation Contains 0.09% sodium azide
UniprotID Q99569 (Human)
Synonym Plakophilin-4, p0071, PKP4
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Ready-to-use
Immunohistochemistry (IHC) - frozen Ready-to-use
Immunohistochemistry (IHC) - paraffin Ready-to-use (microwave treatment recommended)
Western Blot (WB) 1:10-1:50
Background

Detects a plaque protein (p0071) specific for a subtype of adherens junctions, with a molecular mass of 135 kDa and is closely related to neurojungin / delta-catenin and ARVC (all belonging to the p120 armadillo subfamily). p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes.
This protein was also described as plakophilin 4.
Reactivity on cultured cell lines: MCF-7, A431, CaCo-2, HT-29, PLC, 16HBE14o, HaCaT, SV80, U333 CG, 3T3, RVF-SMC, MH1C1
Positive control: MCF-7 cells

References/Publications (3)
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