anti-p34cdc-2 mouse monoclonal, POH-1, ascites fluid
- Mouse monoclonal
- Suitable for IHC on paraffin sections and WB
- Reacts with human
- Isotype: IgG2a
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Purified recombinant human p34 cdc-2 fusion protein|
|Formulation||Contains 0.09% sodium azide|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - paraffin||1:20-1:40 (microwave treatment recommended)|
|Western Blot (WB)||Assay dependent|
The antibody detects the three bands within the 34kD region corresponding to the p34 protein and it's cleavage products. P34cdc-2 is activated and initiates the entry of cells into mitosis, where it acts to promote nuclear envelope breakdown. Expression of p34cdc-2 reflects the proliferating potential of cells.
Positive control: HeLa cells, tonsil or lymph node.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at firstname.lastname@example.org.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.