- Mouse monoclonal
- Suitable for ICC/IF and IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG2a
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG2a |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC |
| Purification | Ascites |
| Reactivity | Human |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | BP53.12 |
| Immunogen | Human recombinant p53 protein |
| Formulation | Contains 0.09% sodium azide |
| UniprotID | Q96A56 (Human) |
| Synonym | Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1, TP53INP1, P53DINP1, SIP |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | Assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5-1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5-1:10 (microwave treatment recommended) |
Background
The gene coding for p53 oncoprotein is located on chromosome 17p. Allele loss at this chromosome site has frequently been seen in many tumours including lung, colon, breast and brain. Expression of p53 oncoprotein was not detected in normal mucosa, whereas mutation results in a detectable expression of the p53 protein. It is therefore suggested that immunocytochemical detection of p53 protein is an indication for malignancy.
Positive control: Breast carcinoma.
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FAQs
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
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