Highly published

anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal + anti-guinea pig IgG HRP set

Cat. No: 71001

Available, delivery time 1-7 days

$439.00*

Key Features
The set provides primary anti-p62 guinea pig polyclonal antibody and secondary anti-guinea pig IgG HRP antibody for Western blot.
The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy.

Included products:

Product description
Quantity 100 µl anti-p62 + 100 µg anti-guinea pig IgG HRP
Antibody Type Polyclonal
Host Goat, Guinea pig
Conjugate Unconjugated
Application IHC, WB
Reactivity See individual antibody datasheet
Storage Lyophilized at 2-8°C; reconstituted at -20°C (avoid freeze/thaw cycles)
Intended use Research use only
Immunogen See individual antibody datasheet for information about specific immunogens
Formulation GP62-C antibody: contains 0.09% sodium azide; anti-guinea pig IgG HRP antibody: lyophilized, reconstitute in 500 µl dist. water (final solution contains 10 mg/ml BSA in PBS buffer, pH 7.4)
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen anti-p62: 1:100-1:600
Immunohistochemistry (IHC) - paraffin anti-p62: 1:100-1:600 (microwave treatment recommended)
Western Blot (WB) anti-p62: 1:1,000-1:3,000; anti-guinea pig HRP: 1:2,500-1:100,000
Background

anti-p62 antibody:
The anti-p62 antibody is useful for research in ubiquitin-associated degradation and autophagy and for detection of neurofibrillary tangles in the brain of Alzheimer disease patients, in Parkinson diseases and various chronic liver diseases.
Human 62 kD (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.

anti-guinea pig IgG goat polyclonal, HRP conjugate:
Whole Guinea Pig IgG secondary antibodies are isolated from antisera by immunoaffinity chromatography. The antibody is conjugated to Horseradish peroxidase (HRP) and is commonly used for WB, IHC and ELISA.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Set content:
Cat. No. GP62-C, anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum
Cat. No. 90001, anti-guinea pig IgG goat polyclonal, HRP conjugate

References/Publications (226)
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FAQs
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
Positive western blot control: anti-p62/SQSTM1 antibody
Cat. No: 64006
  • Whole cell lysate from PLC human hepatoma cell line (PLC/PRF/5)
  • Validated and tested western blot control for: anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal antibody, Cat. No. GP62-C

$85.00*
anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal, serum
Cat. No: GP62-C
  • Guinea pig polyclonal
  • Suitable for IHC and WB
  • Reacts with bovine, human, mouse and rat

$343.00*