anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal, serum

Cat. No: GP62-C

Available, delivery time: 1-7 days

$343.00*

Key Features

Guinea pig polyclonal
Suitable for IHC and WB
Reacts with bovine, human, mouse and rat

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	IHC, WB
Purification	Stabilized antiserum
Reactivity	Bovine, Human, Mouse, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Formulation	Contains 0.09% sodium azide
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100-1:600
Immunohistochemistry (IHC) - paraffin	1:100-1:600 (microwave treatment recommended)
Western Blot (WB)	1:500-1:2,000

Background

Human 62 kDa (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.

The GP62-C antibody is knockout validated (Waguri & Komatsu, 2009).

Waguri, S. & Komatsu, M. Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice. Methods in Enzymology 453, 181-196 (2009).

References/Publications (356)

Publication

Species

Application

Publication Kim, Y. et al. A surge of cytosolic calcium dysregulates lysosomal function and impairs autophagy flux during cupric chloride–induced neuronal death. Journal of Biological Chemistry 300, (2024).

Species mouse

Publication Erb, M. L., Sipple, K., Levine, N., Chen, X. & Moore, D. J. Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities. NPJ Parkinsons Dis 10, 1–12 (2024).

Species mouse

Publication Haugli, K. H., Alkarra, D. & Samuelsen, J. T. Digital manufacturing techniques and the in vitro biocompatibility of acrylic-based occlusal device materials. Clin Oral Investig 28, (2024).

Species human

Publication Vazquez-Sanchez, S. et al. Frontotemporal dementia-like disease progression elicited by seeded aggregation and spread of FUS. Mol Neurodegener 19, (2024).

Application IF

Publication Xu, Z. et al. LKB1 depletion-mediated epithelial–mesenchymal transition induces fibroblast activation in lung fibrosis. Genes Dis 11, (2024).

Species human

Publication Schollmeier, A., Basic, M., Glitscher, M. & Hildt, E. The impact of HBx protein on mitochondrial dynamics and associated signaling pathways strongly depends on the hepatitis B virus genotype. J Virol 98, (2024).

Species human

Application IF

Publication Liao, Y. et al. UBAP2L ensures homeostasis of nuclear pore complexes at the intact nuclear envelope. Journal of Cell Biology 223, (2024).

Species human

Publication Nähse, V. et al. ATPase activity of DFCP1 controls selective autophagy. Nat Commun 14, (2023).

Species human

Application ICC-IF

Publication Sebti, S., Zou, Z. & Shiloh, M. U. BECN1F121A mutation increases autophagic flux in aged mice and improves aging phenotypes in an organ-dependent manner. Autophagy 19, 957–965 (2023).

Species mouse

Publication Hatsuda, A. et al. Calcium signals tune AMPK activity and mitochondrial homeostasis in dendrites of developing neurons. Development (Cambridge) 150, (2023).

Species mouse

Publication Kurashige, T. et al. Characterization of metabolic reprogramming by metabolomics in the oncocytic thyroid cancer cell line XTC.UC1. Sci Rep 13, 149 (2023).

Application WB

Publication Zanuttigh, E. et al. Identification of Autophagy as a Functional Target Suitable for the Pharmacological Treatment of Mitochondrial Membrane Protein-Associated Neurodegeneration (MPAN) In Vitro. Pharmaceutics 15, (2023).

Application WB

Publication Gund, R. & Christiano, A. M. Impaired autophagy promotes hair loss in the C3H/HeJ mouse model of alopecia areata. Autophagy 19, 296–305 (2023).

Species mouse

Application WB

Publication Gonzalez-Sepulveda, M. et al. In vivo reduction of age-dependent neuromelanin accumulation mitigates features of Parkinson’s disease. Brain 146, 1040–1052 (2023).

Species rat

Publication Hegdekar, N. et al. Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes. Autophagy 19, 2026–2044 (2023).

Species mouse

Publication Roşianu, F. et al. Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration. Life Sci Alliance 6, (2023).

Application IF

Publication Rocha, S. M. et al. Microglia-specific knock-out of NF-κB/IKK2 increases the accumulation of misfolded α-synuclein through the inhibition of p62/sequestosome-1-dependent autophagy in the rotenone model of Parkinson’s disease. Glia 71, 2154–2179 (2023).

Species mouse

Publication Silvis, M. R. et al. MYC-mediated resistance to trametinib and HCQ in PDAC is overcome by CDK4/6 and lysosomal inhibition. Journal of Experimental Medicine 220, (2023).

Application IHC

Publication Yonenobu, Y. et al. Neuropathology of spinocerebellar ataxia type 8: Common features and unique tauopathy. Neuropathology 43, 351–361 (2023).

Species human

Publication Gambarotto, L. et al. Sustained oral spermidine supplementation rescues functional and structural defects in COL6-deficient myopathic mice. Autophagy 19, 3221–3229 (2023).

Species mouse

Application WB

Publication Nakamura, J. et al. TFEB-mediated lysosomal exocytosis alleviates high-fat diet-induced lipotoxicity in the kidney. JCI Insight 8, (2023).

Species mouse

Application IHC

Publication Berning, L. et al. The Golgi stacking protein GRASP55 is targeted by the natural compound prodigiosin. Cell Communication and Signaling 21, (2023).

Species human

Application WB

Publication Mitsui, S., Yamaguchi, J., Suzuki, C., Uchiyama, Y. & Tanida, I. TUNEL-positive structures in activated microglia and SQSTM1/p62-positive structures in activated astrocytes in the neurodegenerative brain of a CLN10 mouse model. Glia 71, 2753–2769 (2023).

Species mouse

Publication Opland C. K. et al. Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity., iScience, 26, 106905, (2023).

Species human, mouse

Application WB

Publication Iannibelli E. et al., VCP-related myopathy: a case series and a review of literature., Acta Myol, 42, 2-13, (2023).

Species Human

Application IHC-IF

Publication Xiaojing Kang Shin Shimada Hiroki Miyahara Keiichi Higuchi Masayuki Mori. BALB.NCT-Cpox nct is a unique mouse model of hereditary coproporphyria., Mol Genet Metab Rep, 35100964, (2023).

Species mouse

Application IHC/IF

Publication Akwa Y. et al., Stimulation of synaptic activity promotes TFEB-mediated clearance of pathological MAPT/Tau in cellular and mouse models of tauopathies., Autophagy, 19, 660-677, (2023).

Species Mouse

Application IHC

Publication Martínez‐martínez, E. et al. A Dual‐Acting Nitric Oxide Donor and Phosphodiesterase 5 Inhibitor Activates Autophagy in Primary Skin Fibroblasts. Int J Mol Sci 23, (2022).

Species human

Publication Pitsch, M., Kant, S., Mytzka, C., Leube, R. E. & Krusche, C. A. Autophagy and endoplasmic reticulum stress during onset and progression of arrhythmogenic cardiomyopathy. Cells 11, 96 (2022).

Application IHC-IF

Publication Orhon, I. et al. Autophagy induction during stem cell activation plays a key role in salivary gland self-renewal. Autophagy 18, 293–308 (2022).

Species mouse

Application ICC-IF

Publication Lee, J. H. et al. Faulty autolysosome acidification in Alzheimer’s disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques. Nat Neurosci 25, 688–701 (2022).

Species mouse

Application WB

Publication Noda, S. et al. Impaired mitochondrial accumulation and Lewy pathology in neuron-specific FBXO7-deficient mice. Mol Brain 15, 54 (2022).

Species mouse

Application IHC

Publication Pellegrini, P. et al. Lack of p62 Impairs Glycogen Aggregation and Exacerbates Pathology in a Mouse Model of Myoclonic Epilepsy of Lafora. Mol Neurobiol 59, 1214–1229 (2022).

Species mouse

Application WB

Publication Kawazoe, T. et al. Myoclonic Epilepsy with Ragged-red Fibers with Intranuclear Inclusions. Internal Medicine 61, 547–552 (2022).

Species human

Application IHC

Publication Kuijpers, M. et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum (Neuron (2021) 109(2) (299–313.e9)(2022).

Species mouse

Application IHC

Publication Wolowczyk, C. et al. NRF2 drives an oxidative stress response predictive of breast cancer. Free Radic Biol Med 184, 170–184 (2022).

Application WB

Publication Kondylis, V. et al. p62 Promotes Survival and Hepatocarcinogenesis in Mice with Liver-Specific NEMO Ablation. Cancers (Basel) 14, (2022).

Application WB

Publication Dalle, S. et al. Preliminary Evidence of Differential Expression of Myogenic and Stress Factors in Skeletal Muscle of Older Adults with Low Muscle Strength. Journals of Gerontology - Series A Biological Sciences and Medical Sciences 77, 1121–1129 (2022).

Species human

Application WB

Publication Kieckhöfer, E. et al. Primary cilia suppress Ripk3-mediated necroptosis. Cell Death Discov 8, 1–12 (2022).

Species mouse

Application WB

Publication Pérez-Martí, A. et al. Reducing lipid bilayer stress by monounsaturated fatty acids protects renal proximal tubules in diabetes. Elife 11, (2022).

Application ICC-IF

Publication Kazakova, D., Shimamura, M., Kurashige, T., Hamada, K. & Nagayama, Y. Re-evaluation of the role of autophagy in thyroid cancer treatment. Endocr J 69, 847–862 (2022).

Species human

Application ICC-IF

Publication Mohan, H. M. et al. RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control. Cellular and Molecular Life Sciences 79, (2022).

Species mouse

Application IHC-IF

Publication Oh, C. K. et al. S-Nitrosylation of p62 Inhibits Autophagic Flux to Promote a-Synuclein Secretion and Spread in Parkinson’s Disease and Lewy Body Dementia. Journal of Neuroscience 42, 3011–3024 (2022).

Application WB

Publication Xing, Y. et al. Stimulating TRPM7 suppresses cancer cell proliferation and metastasis by inhibiting autophagy. Cancer Lett 525, 179–197 (2022).

Species human

Application WB

Publication Valionyte, E. et al. The caspase-6–p62 axis modulates p62 droplets based autophagy in a dominant-negative manner. Cell Death Differ 29, 1211–1227 (2022).

Application WB

Publication von Hofsten, S. et al. The marine natural product mimic MPM-1 is cytolytic and induces DAMP release from human cancer cell lines. Sci Rep 12, (2022).

Species human

Application ICC-IF

Publication Brown, M. R. et al. The nuclear receptor REV-ERBα is implicated in the alteration of β-cell autophagy and survival under diabetogenic conditions. Cell Death Dis 13, 1–12 (2022).

Application WB

Publication Sango, J. et al. USP10 inhibits the dopamine-induced reactive oxygen species-dependent apoptosis of neuronal cells by stimulating the antioxidant Nrf2 activity. Journal of Biological Chemistry 298, (2022).

Species human

Application ICC-IF

Publication Yamada, T. et al. Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver. iScience 25, 103996 (2022).

Species Mouse

Application IHC-Fr-IF

Publication Kiryu-Seo, S. et al. Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons., EMBO J 41, e110486, (2022)

Species mouse

Application IHC-IF

Publication Kataura, T. et al. NDP52 acts as a redox sensor in PINK1/Parkin‐mediated mitophagy. EMBO J. e111372 (2022).

Species human

Application WB

Publication Aber E. R. et al., Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and death., Cell Rep, 41, 111480, (2022).

Species Mouse

Application IHC-IF

Publication Gozzelino L. et al., Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy., Brain, 145, 2313-2331, (2022).

Species Mouse

Application IHC-IF

Publication Abdullah C. S. et al. Mitochondrial dysfunction and autophagy activation are associated with cardiomyopathy developed by extended methamphetamine self-administration in rats., Redox Biol, 58, 102523, (2022).

Species rat

Application WB

Publication Jackson, J. et al. SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells. Mol. Metab. 61, 101503 (2022).

Species Human

Application WB

Publication Schmitt D. et al. Lipid and protein content profiling of isolated native autophagic vesicles., EMBO Rep, 23, e53065, (2022).

Species human

Application ICC, WB

Publication Li, Y. et al. Impairment of autophagy after spinal cord injury potentiates neuroinflammation and motor function deficit in mice., Theranostics 12, 5364-5388, (2022).

Species mouse

Application IHC

Publication Varea, O., Guinovart, J. J. & Duran, J. Malin restoration as proof of concept for gene therapy for Lafora disease. Brain Commun. 4, (2022).

Species mouse

Application IHC-IF (paraffin)

Publication Suzuki, C., Yamaguchi, J., et al. Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins. Sci Rep. (2022).

Species Mouse

Application IHC

Publication Solvik, T. A. et al. Secretory autophagy maintains proteostasis upon lysosome inhibition., J Cell Biol 221, (2022).

Species human

Application ICC-IF, WB

Publication Sharma, K. et al. Autophagy modulates cell fate decisions during lineage commitment., Autophagy 18, 1915-1931, (2022).

Species human

Application WB

Publication De Mazière A. et al. An optimized protocol for immuno-electron microscopy of endogenous LC3., Autophagy, 18, 3004-3022, (2022).

Species Human

Application EM

Publication Hess, M. W. et al. Advanced microscopy for liver and gut ultrastructural pathology in patients with mvid and pfic caused by myo5b mutations. J Clin Med 10, (2021).

Species human

Application IHC(frozen)-IF

Publication Srimat Kandadai, K. et al. ATG5 in microglia does not contribute vitally to autoimmune neuroinflammation in mice. Autophagy 17, 3566–3576 (2021).

Species mouse

Publication Arcones, A. C. et al. Cardiac grk2 protein levels show sexual dimorphism during aging and are regulated by ovarian hormones. Cells 10, 1–14 (2021).

Species mouse

Application WB

Publication Mochida, A. et al. Defective autophagy in vascular smooth muscle cells enhances the healing of abdominal aortic aneurysm. Physiol Rep 9, (2021).

Species mouse

Application WB

Publication Somlapura, M. et al. Different roles of p62 (Sqstm1) isoforms in keratin‐related protein aggregation. Int J Mol Sci 22, 6227 (2021).

Species human, chinese hamster

Application WB, ICC-IF

Publication Fukuda, H. et al. Father-to-offspring transmission of extremely long NOTCH2NLC repeat expansions with contractions: genetic and epigenetic profiling with long-read sequencing. Clin Epigenetics 13, 204 (2021).

Species human

Application ICC-IF

Publication Sun, Y. et al. Fin56-induced ferroptosis is supported by autophagy-mediated GPX4 degradation and functions synergistically with mTOR inhibition to kill bladder cancer cells. Cell Death Dis 12, 1–14 (2021).

Species mouse

Application WB

Publication Cabezudo, S. et al. Gαq activation modulates autophagy by promoting mTORC1 signaling. Nat Commun 12, 1–19 (2021).

Species human, mouse, rat

Application IP

Publication Glitscher, M. et al. Identification of the Interferon-Inducible GTPase GBP1 as a Major Restriction Factor for Hepatitis E Virus. J Virol 95, (2021).

Species human

Publication Makino, S. I. et al. Impairment of proteasome function in podocytes leads to CKD. Journal of the American Society of Nephrology 32, 597–613 (2021).

Species mouse

Application WB, IHC

Publication Tanaka, Y. et al. LPIAT1/MBOAT7 depletion increases triglyceride synthesis fueled by high phosphatidylinositol turnover. Gut 70, 180–193 (2021).

Species human

Application WB

Publication Qi, J. et al. MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx. Autophagy 17, 4401–4422 (2021).

Species mouse

Application IHC

Publication Sekiguchi, K. et al. Metabolome characteristics of liver autophagy deficiency under starvation conditions in infancy. Nutrients 13, (2021).

Species mouse

Application WB

Publication Xian, H. et al. Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation. Immunity 54, 1463-1477.e11 (2021).

Species mouse

Application WB

Publication Huang, J. et al. NBR1 is a critical step in the repression of thermogenesis of p62-deficient adipocytes through PPARγ. Nat Commun 12, 1–13 (2021).

Species human, mouse

Application IP, WB

Publication Kageyama, S. et al. p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response. Nat Commun 12, 1–16 (2021).

Species human

Application WB

Publication Berning, L. et al. Prodigiosin sensitizes sensitive and resistant urothelial carcinoma cells to cisplatin treatment. Molecules 26, (2021).

Species human

Application WB

Publication Franekova, V., Storjord, H. I., Leivseth, G. & Nilssen, Ø. Protein homeostasis in LGMDR9 (LGMD2I) – The role of ubiquitin–proteasome and autophagy–lysosomal system. Neuropathol Appl Neurobiol 47, 519–531 (2021).

Species human

Application IF

Publication Besemer, A. S. et al. Receptor-mediated endocytosis 8 (RME-8)/DNAJC13 is a novel positive modulator of autophagy and stabilizes cellular protein homeostasis. Cellular and Molecular Life Sciences 78, 645–660 (2021).

Application ICC, WB

Publication Straka, T. et al. Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor. Front Physiol 12, (2021).

Species mouse

Application WB, IHC(frozen)

Publication He, X. et al. TFE3-Mediated Autophagy is Involved in Dopaminergic Neurodegeneration in Parkinson’s Disease. Front Cell Dev Biol 9, (2021).

Species mouse

Application IHC(frozen)-IF

Publication Coudert, L., Osseni, A., Gangloff, Y. G., Schaeffer, L. & Leblanc, P. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways. BMC Biol 19, (2021).

Application WB, IF

Publication Yuan, T. et al. The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis. Nat Commun 12, (2021).

Species human, mouse, rat

Application IHC, WB

Publication Wu, W. et al. TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy. Autophagy 17, 3992–4009 (2021).

Species mouse

Application WB

Publication Hegdekar, N., Lipinski, M. M. & Sarkar, C. N-Acetyl-l-leucine improves functional recovery and attenuates cortical cell death and neuroinflammation after traumatic brain injury in mice. Sci. Rep. 11, (2021).

Species mouse

Application IHC-IF

Publication Sargent, D. et al. Neuronal VPS35 deletion induces spinal cord motor neuron degeneration and early post-natal lethality . Brain Commun. 3, 1–20 (2021).

Species Mouse

Application IHC-IF

Publication Deitersen, J. et al. High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol. Cell Death Dis. 12, (2021).

Species human

Application WB

Publication Schmitz, K. et al. An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis. Nucleic Acids Res. 49, 6437–6455 (2021).

Species Human

Application WB

Publication Nozaki, M. et al. SQSTM1L341V variant that is linked to sporadic ALS exhibits impaired association with MAP1LC3 in cultured cells. eNeurologicalSci. 22, 100301(2021).

Species mouse

Application ICC/IF

Publication Cui, Y. H. et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat. Commun. 12, 1–19 (2021).

Species human

Application WB

Publication Schlütermann, D. et al. FIP200 controls the TBK1 activation threshold at SQSTM1/p62-positive condensates. Sci. Rep. 11, (2021).

Species mouse

Application WB

Publication Nthiga, T. M. et al. Regulation of golgi turnover by CALCOCO1-mediated selective autophagy. J. Cell Biol. 220, (2021).

Species Human

Application WB

Publication Sønder, S.L. et al. Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis. Sci Adv. 7, NULL(2021).

Species human

Application WB

Publication Mercer, T. J. et al. Phosphoproteomic identification of ULK substrates reveals VPS15‐dependent ULK/VPS34 interplay in the regulation of autophagy. EMBO J. 40, (2021).

Species human

Application ICC-IF, WB

Publication Goodwin, J.M. et al. GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis. Sci Adv. 7, eabj2485(2021).

Species human

Application WB

Publication Rudnick, J. A. et al. Autophagy in stromal fibroblasts promotes tumor desmoplasia and mammary tumorigenesis. Genes Dev. 35, 963–975 (2021).

Species Mouse

Application IHC-P

Publication Kawakami, I. et al. Progression of phosphorylated α-synuclein in Macaca fuscata. Brain Pathol. 31, e12952(2021).

Species macaque

Application IHC (paraffin)

Publication Marsh, T. et al. Autophagic Degradation of NBR1 Restricts Metastatic Outgrowth during Mammary Tumor Progression. Dev Cell 52, 591-604.e6 (2020).

Species mouse

Application WB

Publication Cruces-Sande, M. et al. Autophagy mediates hepatic GRK2 degradation to facilitate glucagon-induced metabolic adaptation to fasting. FASEB Journal 34, 399–409 (2020).

Species mouse

Application WB

Publication Nakazawa, Y. et al. Degradation of connexin 50 protein causes waterclefts in human lens. Open Medicine 15, 1163–1171 (2020).

Species human

Application WB

Publication Zhou, H. et al. Disruption of Atg7-dependent autophagy causes electromotility disturbances, outer hair cell loss, and deafness in mice. Cell Death Dis 11, (2020).

Species mouse

Application IHC, WB

Publication Barroso-Chinea, P. et al. DRD3 (dopamine receptor D3) but not DRD2 activates autophagy through MTORC1 inhibition preserving protein synthesis. Autophagy 16, 1279–1295 (2020).

Species mouse

Application WB

Publication Miki, T. et al. Factors associated with development and distribution of granular/fuzzy astrocytes in neurodegenerative diseases. Brain Pathology 30, 811–830 (2020).

Species human

Application IHC

Publication Schregle, R. et al. FAT10 localises in dendritic cell aggresome-like induced structures and contributes to their disassembly. J Cell Sci 133, (2020).

Species mouse

Application WB

Publication Chastagner, P. et al. Fate and propagation of endogenously formed Tau aggregates in neuronal cells. EMBO Mol Med 12, (2020).

Species mouse

Application ICC-IF

Publication Homma, K. et al. Genome-wide siRNA screening reveals that DCAF4-mediated ubiquitination of optineurin stimulates autophagic degradation of Cu,Zn-superoxide dismutase. Journal of Biological Chemistry 295, 3148–3158 (2020).

Species human

Application WB

Publication Zhang, X. et al. High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy. Nat Metab 2, 110–125 (2020).

Species mouse

Application ICC-IF, IHC(frozen)-IF, IHC-IF

Publication Bojko, A. et al. Improved autophagic flux in escapers from doxorubicin-induced senescence/polyploidy of breast cancer cells. Int J Mol Sci 21, 1–22 (2020).

Species human

Publication ASAHINA, M. et al. JF1/B6F1 Ngly1–/– mouse as an isogenic animal model of NGLY1 deficiency. Proc Jpn Acad Ser B Phys Biol Sci 97, 89–102 (2020).

Species mouse

Application IHC-IF

Publication Sharkey, L. M. et al. Modeling UBQLN2-mediated neurodegenerative disease in mice: Shared and divergent properties of wild type and mutant UBQLN2 in phase separation, ... Neurobiol Dis 143, (2020).

Species mouse

Application IHC-IF, WB

Publication Bork, T. et al. Podocytes maintain high basal levels of autophagy independent of mtor signaling. Autophagy 16, 1932–1948 (2020).

Species mouse

Application WB, IHC(paraffin), IHC-IF

Publication Dumas, K. et al. REDD1 deficiency protects against nonalcoholic hepatic steatosis induced by high-fat diet. FASEB Journal 34, 5046–5060 (2020).

Species mouse

Application WB

Publication Goldsmith, J. et al. Ribosome profiling reveals a functional role for autophagy in mRNA translational control. Commun Biol 3, (2020).

Species mouse

Application WB

Publication Aoyama, S. et al. Rubicon in pancreatic beta cells plays a limited role in maintaining glucose homeostasis following increased insulin resistance. Endocr J 67, 1119–1126 (2020).

Species mouse

Application WB

Publication Wang, Y. T. et al. Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes. Nature Microbiology vol. 5 272–281 Preprint at https://doi.org/10.1038/s41564-019-0633-0 (2020).

Species mouse

Application ICC-IF

Publication Solhaug, A. et al. The Fusarium mycotoxin, 2-Amino-14,16-dimethyloctadecan-3-ol (AOD) induces vacuolization in HepG2 cells. Toxicology 433–434, (2020).

Species human

Application WB

Publication Lee, J. H. et al. β2-adrenergic Agonists Rescue Lysosome Acidification and Function in PSEN1 Deficiency by Reversing Defective ER-to-lysosome Delivery of ClC-7. Journal of Molecular Biology vol. 432 2633–2650 (2020).

Species mouse

Publication Fucci, C. et al. The Interaction of the Tumor Suppressor FAM46C with p62 and FNDC3 Proteins Integrates Protein and Secretory Homeostasis. Cell Rep. 32, 108162(2020).

Species human

Application ICC/IF

Publication Metti, S. et al. The Polyphenol Pterostilbene Ameliorates the Myopathic Phenotype of Collagen VI Deficient Mice via Autophagy Induction. Front. Cell Dev. Biol. 8, (2020).

Species mouse

Application IHC-IF

Publication Keller, F. et al. A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells 9, (2020).

Species human

Application ICC

Publication Deng, Z. et al. ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway. Autophagy. 16, 917-931 (2020)

Species human,mouse

Application WB

Publication Noman, M. et al. Inhibition of Vps34 reprograms cold into hot inflamed tumors and improves anti-PD-1/PD-L1 immunotherapy. Sci.Adv. 6, eaax7881 (2020)

Species mouse

Application WB

Publication Kurashige, T. et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J.Endocr.Soc. 4, bvaa054 (2020)

Species rat

Application WB,IHC-IF (paraffin)

Publication Manzetti, J. et al. BK Polyomavirus Evades Innate Immune Sensing by Disrupting the Mitochondrial Network and Promotes Mitophagy. iScience. 23, 101257(2020).

Species human

Application IHC (paraffin)/IF

Publication Jakobi, A. et al. Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake. Nat.Commun. 11, 440 (2020)

Species human

Application WB

Publication Saegusa, C. et al. Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells.. Laryngoscope Investig Otolaryngol. 5, 520-528(2020).

Species mouse

Application IHC/IF

Publication Werner, G. et al. Loss of TMEM106B potentiates lysosomal and FTLD-like pathology in progranulin-deficient mice. EMBO Rep. 21, e50241(2020).

Species mouse

Application IHC/IF

Publication Baeken, M. W. et al. Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Cells 9, (2020).

Species human

Application ICC-IF, WB

Publication Kakimoto, Y. et al. Myocardial cathepsin D is downregulated in sudden cardiac death. PLoS.One. 15, e0230375 (2020)

Species human

Application WB

Publication Aishwarya, R. et al. Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytes. Redox Biol. 36, 101660(2020).

Species rat

Application WB

Publication Leidal, A. et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat.Cell.Biol. 22, 187-199 (2020)

Species human

Application WB

Publication Bhushan, J. et al. Isg15 connects autophagy and ifn-dependent control of toxoplasma gondii infection in human cells. MBio 11, 1–19 (2020).

Species human

Application ICC-IF, WB

Publication Dubois, C. et al. Co-targeting Mitochondrial Ca2+ Homeostasis and Autophagy Enhances Cancer Cells' Chemosensitivity. iScience. 23, 101263(2020).

Species human

Application WB

Publication Choi, I. et al. Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration. Nat.Commun. 11, 1386 (2020)

Species mouse

Application ICC-IF

Publication Takahashi, S. et al. Loss of autophagy impairs physiological steatosis by accumulation of NCoR1. Life.Sci.Alliance. 3, (2020)

Species mouse

Application WB

Publication Dohmen, M. et al. AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nat.Commun. 11, 1032 (2020)

Species mouse

Application WB

Publication Blázquez, C. et al. Inhibition of striatonigral autophagy as a link between cannabinoid intoxication and impairment of motor coordination. Elife 9, 1–24 (2020).

Species mouse

Application WB

Publication Paha, J. et al. A novel potent autophagy inhibitor ECDD-S27 targets vacuolar ATPase and inhibits cancer cell survival. Sci Rep 9, 1–16 (2019).

Species human, mouse

Application WB

Publication Malvin, N. P., Kern, J. T., Liu, T. C., Brody, S. L. & Stappenbeck, T. S. Autophagy proteins are required for club cell structure and function in airways. Am J Physiol Lung Cell Mol Physiol 317, L259–L270 (2019).

Species mouse

Application IHC(paraffin)-IF

Publication Mohamud, Y. et al. CALCOCO2/NDP52 and SQSTM1/p62 differentially regulate coxsackievirus B3 propagation. Cell Death Differ 26, 1062–1076 (2019).

Species human

Application WB, ICC-IF

Publication Wu, Z. et al. MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure. Mol Cell 75, 835-848.e8 (2019).

Species human

Application ICC-IF

Publication Princely Abudu, Y. et al. NIPSNAP1 and NIPSNAP2 Act as “Eat Me” Signals for Mitophagy. Dev Cell 49, 509-525.e12 (2019).

Species human

Application IHC-IF

Publication Ivanova, S. et al. Regulation of death receptor signaling by the autophagy protein TP 53 INP 2 . EMBO J 38, (2019).

Species mouse

Application WB

Publication Lau, N. et al. SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole. PLoS Pathog 15, (2019).

Species human

Application ICC-IF

Publication Cavaliere, F. et al. The tricyclic antidepressant clomipramine inhibits neuronal autophagic flux. Sci Rep 9, (2019).

Species mouse

Application WB

Publication Lee, J. H. et al. Transgenic expression of a ratiometric autophagy probe specifically in neurons enables the interrogation of brain autophagy in vivo. Autophagy 15, 543–557 (2019).

Species mouse

Application WB

Publication Glatigny, M. et al. Autophagy Is Required for Memory Formation and Reverses Age-Related Memory Decline. Curr.Biol. 29, 435-448.e8 (2019)

Species mouse

Application WB,IHC-IF (frozen)

Publication Gorgulu, K. et al. Levels of the Autophagy-Related 5 Protein Affect Progression and Metastasis of Pancreatic Tumors in Mice. Gastroenterology. 156, 203-217.e20 (2019)

Species mouse

Application WB

Publication Lim, J. et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. Elife. 8, (2019)

Species mouse

Application WB

Publication Gasset-Rosa, F. et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. Neuron. 102, 339-357.e7 (2019)

Species human

Application ICC-IF

Publication Bonsi, P. et al. RGS9-2 rescues dopamine D2 receptor levels and signaling in DYT1 dystonia mouse models. EMBO.Mol.Med. 11, (2019)

Species mouse

Application WB

Publication Zhang, J. et al. Cross-regulation of defective endolysosome trafficking and enhanced autophagy through TFEB in UNC13D deficiency. Autophagy. , 1-19 (2019)

Species mouse

Application WB

Publication Kessler, S. et al. Diethylnitrosamine (DENA) recapitulates formation of hepatic angiosarcoma in pigs. PLoS.One. 14, e0214756 (2019)

Species pig

Application IHC (paraffin)

Publication Rienzo, M. et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains. Sci.Adv. 5, eaau8857 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Sánchez-López, E. et al. Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production. Cell.Metab. 29, 1350-1362.e7 (2019)

Species mouse

Application ICC

Publication Horigome, Y. et al. Loss of autophagy in chondrocytes causes severe growth retardation. Autophagy. , 1-11 (2019)

Species mouse

Application IHC (paraffin)

Publication Javaheri, A. et al. TFEB activation in macrophages attenuates postmyocardial infarction ventricular dysfunction independently of ATG5-mediated autophagy. JCI.Insight. 4, (2019)

Species human

Application IHC (paraffin)

Publication Hill, C. et al. Autophagy inhibition-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in pulmonary fibrosis. Cell.Death.Dis. 10, 591 (2019)

Species human

Application IHC (paraffin)

Publication Kinsey, C. et al. Protective autophagy elicited by RAF→MEK→ERK inhibition suggests a treatment strategy for RAS-driven cancers. Nat.Med. 25, 620-627 (2019)

Species mouse

Application IHC (paraffin)

Publication Schwob, A. et al. SQSTM-1/p62 potentiates HTLV-1 Tax-mediated NF-κB activation through its ubiquitin binding function. Sci.Rep. 9, 16014 (2019)

Species human

Application IF,WB

Publication Nam, S. et al. Autophagy attenuates tubulointerstital fibrosis through regulating transforming growth factor-β and NLRP3 inflammasome signaling pathway. Cell.Death.Dis. 10, 78 (2019)

Species mouse

Application WB,IHC (paraffin)

Publication Saito, T. et al. Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nat.Commun. 10, 1567 (2019)

Species mouse

Application WB

Publication Aubert, P. et al. Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring. FASEB.J. 33, 770-781 (2019)

Species rat

Application WB

Publication Yang, Y. et al. Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response. Nat.Commun. 10, 3759 (2019)

Species human

Application WB

Publication Sarkar, C. et al. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma. Autophagy. , 1-20 (2019)

Species mouse

Application ICC

Publication Jung, S. et al. Buffering of cytosolic calcium plays a neuroprotective role by preserving the autophagy-lysosome pathway during MPP+-induced neuronal death. Cell.Death.Discov. 5, 130 (2019)

Species mouse

Application WB

Publication Overå, K. et al. TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1. J.Cell.Sci. 132, (2019)

Species human

Application WB

Publication Riemschoss, K. et al. Fibril-induced glutamine-/asparagine-rich prions recruit stress granule proteins in mammalian cells. Life.Sci.Alliance. 2, (2019)

Species mouse

Application WB,ICC-IF

Publication Birgisdottir, Å. et al. Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs. Autophagy. , Jan-23 (2019)

Species human

Application WB,IHC,IP

Publication Pellegrini, C. et al. Altered adipocyte differentiation and unbalanced autophagy in type 2 Familial Partial Lipodystrophy: an in vitro and in vivo study of adipose tissue browning. Exp.Mol.Med. 51, 89 (2019)

Species human

Application WB,ICC-IF

Publication Sánchez-López, E. et al. Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production. Cell.Metab. 29, 1350-1362.e7 (2019)

Species mouse

Application ICC

Publication Tang, T. et al. Dihydroartemisinin and its anticancer activity against endometrial carcinoma and cervical cancer: involvement of apoptosis, autophagy and transferrin receptor. Singapore.Med.J. , (2019)

Species human

Application WB

Publication Chakraborty, D. et al. Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress. Redox.Biol. 24, 101181 (2019)

Species mouse

Application WB

Publication Wang, Y. et al. Autophagy inhibition specifically promotes epithelial-mesenchymal transition and invasion in RAS-mutated cancer cells. Autophagy. , Jan-14 (2019)

Species mouse

Application ICC-IF,WB

Publication Park, S. et al. A lysosomal chloride ion-selective fluorescent probe for biological applications. Chem.Sci. 10, 56-66 (2019)

Species human

Application ICC-IF

Publication Gurlo, T. et al. Pregnancy in human IAPP transgenic mice recapitulates beta cell stress in type 2 diabetes. Diabetologia. 62, 1000-1010 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Carballo-Carbajal, I. et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat.Commun. 10, 973 (2019)

Species rat

Application WB,IHC (paraffin)

Publication Igawa, H. et al. p62-mediated autophagy affects nutrition-dependent insulin receptor substrate 1 dynamics in 3T3-L1 preadipocytes. J.Diabetes.Investig. 10, 32-42 (2019)

Species mouse

Application WB

Publication Himuro, M. et al. Cellular Autophagy in α Cells Plays a Role in the Maintenance of Islet Architecture. J.Endocr.Soc. 3, 1979-1992 (2019)

Species mouse

Application IHC (paraffin)

Publication Castets, P. et al. mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4. Nat.Commun. 10, 3187 (2019)

Species mouse

Application IHC (paraffin)

Publication Klimek, C. et al. The Hippo network kinase STK38 contributes to protein homeostasis by inhibiting BAG3-mediated autophagy. Biochim. Biophys. Acta - Mol. Cell Res. 1866, 1556–1566 (2019).

Species human

Application WB

Publication Ma, X. et al. Transcription Factor EB Activation Rescues Advanced αB-Crystallin Mutation-Induced Cardiomyopathy by Normalizing Desmin Localization. J.Am.Heart.Assoc. 8, e010866 (2019)

Species mouse

Application IHC (paraffin)

Publication Kim, W. et al. Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia. Sci.Rep. 9, 3021 (2019)

Species mouse

Application IHC (paraffin)

Publication Yang, S. et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nat.Commun. 10, 2782 (2019)

Species human

Application WB

Publication Liang, S. et al. Murine macrophage autophagy protects against alcohol-induced liver injury by degrading interferon regulatory factor 1 (IRF1) and removing damaged mitochondria. J.Biol.Chem. , (2019)

Species mouse

Application WB

Publication Tamura, N. et al. Hyperosmotic stress induces unconventional autophagy independent of the Ulk1 complex. Mol.Cell.Biol. , (2019)

Species human,mouse

Application WB,ICC

Publication Pigna, E. et al. Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosis. EBioMedicine. 40, 717-732 (2019)

Species mouse

Application IHC-IF (frozen)

Publication Garcia, D. et al. Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell.Rep. 26, 192-208.e6 (2019)

Species mouse

Application WB

Publication Vanzo, R. et al. Autophagy role(s) in response to oncogenes and DNA replication stress. Cell.Death.Differ. , (2019)

Species human

Application WB

Publication Lachance, V. et al. Autophagy protein NRBF2 has reduced expression in Alzheimer's brains and modulates memory and amyloid-beta homeostasis in mice. Mol.Neurodegener. 14, 43 (2019)

Species mouse

Application WB

Publication Anisimov, S. et al. G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10. Sci.Rep. 9, 12896 (2019)

Species human

Application WB

Publication Pasquier, A. et al. Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes. Nat.Commun. 10, 3312 (2019)

Species mouse

Application ICC-IF

Publication Sabino, C. et al. Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection. Viruses. 11, (2019)

Species human

Application WB

Publication Olivé, M. et al. Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions. Nat.Commun. 10, 1396 (2019)

Species human

Application IHC (frozen)

Publication Hoem, G. et al. The FMRpolyGlycine Protein Mediates Aggregate Formation and Toxicity Independent of the CGG mRNA Hairpin in a Cellular Model for FXTAS. Front.Genet. 10, 249 (2019)

Species human

Application ICC-IF

Publication Sarraf, S. et al. PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Cell.Rep. 29, 225-235.e5 (2019)

Species mouse

Application WB

Publication Aghajan, M. et al. Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice. JCI.Insight. 4, (2019)

Species mouse

Application WB

Publication Li, Y. et al. cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury. Cell.Death.Dis. 10, 531 (2019)

Species mouse

Application IHC (frozen)

Publication Suzuki, C. et al. Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions. Int.J.Mol.Sci. 21, (2019)

Species mouse

Application ICC-IF

Publication Samdal, H. et al. Basal level of autophagy and MAP1LC3B-II as potential biomarkers for DHA-induced cytotoxicity in colorectal cancer cells. FEBS Journal 285, 2446–2467 (2018).

Species human

Application WB

Publication Masuyama, A. et al. Defective autophagy in vascular smooth muscle cells enhances atherosclerotic plaque instability. Biochem Biophys Res Commun 505, 1141–1147 (2018).

Species mouse

Application IHC

Publication Yin, Y. et al. Effects of DHA on Hippocampal Autophagy and Lysosome Function After Traumatic Brain Injury. Mol Neurobiol 55, 2454–2470 (2018).

Species rat

Publication Shi, M. et al. Effects of erythropoietin receptor activity on angiogenesis, tubular injury, and fibrosis in acute kidney injury: A “U-shaped” relationship. Am J Physiol Renal Physiol 314, F501–F516 (2018).

Species mouse

Application WB

Publication Ripolone, M. et al. Effects of short-to-long term enzyme replacement therapy (ERT) on skeletal muscle tissue in late onset Pompe disease (LOPD). Neuropathol Appl Neurobiol 44, 449–462 (2018).

Species human

Application WB

Publication Kim, J. Y. et al. ER Stress Drives Lipogenesis and Steatohepatitis via Caspase-2 Activation of S1P. Cell 175, 133-145.e15 (2018).

Species mouse

Application IHC

Publication Kivinen, N. The role of autophagy in age-related macular degeneration. Acta.Ophthalmol. 96 Suppl A110, Jan-50 (2018)

Species mouse

Application IHC (paraffin)

Publication Osonoi, Y. et al. Defective autophagy in vascular smooth muscle cells enhances cell death and atherosclerosis. Autophagy. 14, 1991-2006 (2018)

Species mouse

Application IHC (frozen)

Publication Mauthe, M. et al. Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy. 14, 1435-1455 (2018)

Species human

Application ICC-IF

Publication Kageyama, S. et al. Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Mol.Cell.Biol. , (2018).

Species mouse

Application WB

Publication Sebori, R. et al. Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice. Oxid.Med.Cell.Longev. 2018, 9179270 (2018).

Species mouse

Application WB

Publication Nwadike, C. et al. AMPK Inhibits ULK1-Dependent Autophagosome Formation and Lysosomal Acidification via Distinct Mechanisms. Mol.Cell.Biol. 38, (2018).

Species mouse

Application ICC

Publication Aron, R. et al. Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease. Nat.Commun. 9, 3191 (2018).

Species rat

Application WB

Publication Battista, R. et al. Autophagy mediates epithelial cancer chemoresistance by reducing p62/SQSTM1 accumulation. PLoS.One. 13, e0201621 (2018).

Species human

Application WB,ICC-IF

Publication Miret-Casals, L. et al. Identification of New Activators of Mitochondrial Fusion Reveals a Link between Mitochondrial Morphology and Pyrimidine Metabolism. Cell.Chem.Biol. 25, 268-278.e4 (2018)

Species human

Application WB

Publication Sato, S. et al. Loss of autophagy in dopaminergic neurons causes Lewy pathology and motor dysfunction in aged mice. Sci.Rep. 8, 2813 (2018)

Species mouse

Application WB,IHC-IF (frozen)

Publication Koyuncu, S. et al. The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. Nat.Commun. 9, 2886 (2018).

Species human

Application WB

Publication McLelland, G. et al. Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy. Elife. 7, (2018).

Species human

Application WB

Publication Vincent, A. et al. Subcellular origin of mitochondrial DNA deletions in human skeletal muscle. Ann.Neurol. 84, 289-301 (2018).

Species human

Application IHC-IF (frozen)

Publication Matta, S. et al. NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages. MBio. 9, (2018).

Species mouse

Application ICC-IF

Publication Viana-Huete, V. et al. Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology. 159, 323-340 (2018)

Species mouse

Application ICC-IF

Publication Fernández, A. et al. Disruption of the beclin 1-BCL2 autophagy regulatory complex promotes longevity in mice. Nature. 558, 136-140 (2018)

Species mouse

Application WB

Publication Sebastiani, A. et al. Sequestosome 1 Deficiency Delays, but Does Not Prevent Brain Damage Formation Following Acute Brain Injury in Adult Mice. Front.Neurosci. 11, 678 (2018).

Species mouse

Application WB

Publication Livingston, J. et al. Analysis of HSP27 and the Autophagy Marker LC3B+ Puncta Following Preoperative Chemotherapy Identifies High-Risk Osteosarcoma Patients. Mol.Cancer.Ther. 17, 1315-1323 (2018)

Species human

Application IHC (paraffin)

Publication Ogmundsdóttir, M. et al. A short isoform of ATG7 fails to lipidate LC3/GABARAP. Sci.Rep. 8, 14391 (2018).

Species mouse

Application WB

Publication Carroll, B. et al. Oxidation of SQSTM1/p62 mediates the link between redox state and protein homeostasis. Nat.Commun. 9, 256 (2018).

Species human,mouse

Application WB

Publication Alam, S. et al. Aberrant Mitochondrial Fission Is Maladaptive in Desmin Mutation-Induced Cardiac Proteotoxicity. J.Am.Heart.Assoc. 7, (2018).

Species mouse

Application WB

Publication Oehl, K. et al. Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression. Front.Oncol. 8, 40 (2018).

Species human

Application WB

Publication Kuno, A. et al. Resveratrol Ameliorates Mitophagy Disturbance and Improves Cardiac Pathophysiology of Dystrophin-deficient mdx Mice. Sci.Rep. 8, 15555 (2018).

Species mouse

Application WB

Publication Wu, Z. et al. Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy. Cell.Metab. 28, 130-144.e7 (2018)

Species drosophila

Application WB

Publication Furuya, N. et al. NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy. EMBO.Rep. 19, (2018)

Species human

Application WB

Publication Cabe, M. et al. PB1 and UBA domains of p62 are essential for aggresome-like induced structure formation. Biochem.Biophys.Res.Commun. 503, 2306-2311 (2018)

Species mouse

Application WB

Publication Takahashi, M. et al. USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience. 9, 433-450 (2018).

Species human

Application WB,ICC-IF

Publication Nikam, A. et al. The PPARα Agonist Fenofibrate Prevents Formation of Protein Aggregates (Mallory-Denk bodies) in a Murine Model of Steatohepatitis-like Hepatotoxicity. Sci.Rep. 8, 12964 (2018).

Species mouse

Application WB

Publication Ziegler, P. et al. Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. Cell. 174, 88-101.e16 (2018)

Species human

Application IHC-IF (paraffin)

Publication Liu, S. et al. Lysosomal damage after spinal cord injury causes accumulation of RIPK1 and RIPK3 proteins and potentiation of necroptosis. Cell.Death.Dis. 9, 476 (2018).

Species mouse

Application IHC (frozen)

Publication Barutcu, S. et al. Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy. Autophagy. 14, 1586-1595 (2018).

Species mouse

Application WB

Publication Prabakaran, T. et al. Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1. EMBO.J. 37, (2018)

Species human,mouse

Application WB,ICC-IF

Publication Mejlvang, J. et al. Starvation induces rapid degradation of selective autophagy receptors by endosomal microautophagy. J.Cell.Biol. 217, 3640-3655 (2018)

Species human

Application WB,ICC-IF

Publication Mitsui, S. et al. Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1H46R-expressing ALS mouse model. Mol.Brain. 11, 30 (2018).

Species mouse

Application IHC-IF (paraffin)

Publication Lim, H. et al. A novel autophagy enhancer as a therapeutic agent against metabolic syndrome and diabetes. Nat.Commun. 9, 1438 (2018).

Species human

Application WB

Publication Yamada, T. et al. Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease. Cell.Metab. , (2018)

Species mouse

Application IHC-IF (paraffin)

Publication McLendon, P. et al. An Unbiased High-Throughput Screen to Identify Novel Effectors That Impact on Cardiomyocyte Aggregate Levels. Circ.Res. 121, 604-616 (2017).

Species rat

Application WB

Publication Shiba-Fukushima, K. et al. Evidence that phosphorylated ubiquitin signaling is involved in the etiology of Parkinson's disease. Hum.Mol.Genet. 26, 3172-3185 (2017)

Species human

Application WB

Publication Shimozawa, A. et al. Propagation of pathological α-synuclein in marmoset brain. Acta Neuropathol. Commun. 5, 12 (2017)

Species monkey

Application ICC-IF

Publication Wallot-Hieke, N. et al. Systematic analysis of ATG13 domain requirements for autophagy induction. Autophagy. , 1-58 (2017)

Species mouse

Application ICC-IF

Publication Gómez-Sintes, R. et al. Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells. 6, (2017)

Species mouse

Application ICC-IF

Publication Nascimbeni, A. C., Fanin, M., Angelini, C. & Sandri, M. Autophagy dysregulation in Danon disease. Cell Death Dis. 8, e2565 (2017).

Species human

Application WB,IHC (frozen)

Publication Sample, A. et al. Adaptor protein p62 promotes skin tumor growth and metastasis and is induced by UVA radiation. J.Biol.Chem. 292, 14786-14795 (2017).

Species human

Application WB

Publication Gallagher, L. et al. Lysosomotropism depends on glucose: a chloroquine resistance mechanism. Cell.Death.Dis. 8, e3014 (2017).

Species mouse

Application WB

Publication Conte, A. et al. High mobility group A1 protein modulates autophagy in cancer cells. Cell.Death.Differ. 24, 1948-1962 (2017).

Species human

Application ICC-IF

Publication Sergin, I. et al. Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosis. Nat. Commun. 8, 15750 (2017).

Species human,mouse

Application WB,IHC (frozen),ICC-IF

Publication Lee, M. Y. et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep. 18, 48–60 (2017).

Species mouse

Application WB

Publication Hartupee, J. et al. Impaired Protein Quality Control During Left Ventricular Remodeling in Mice With Cardiac Restricted Overexpression of Tumor Necrosis Factor. Circ.Heart.Fail. 10, (2017).

Species mouse

Application IHC-IF (paraffin)

Publication Giaime, E. et al. Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice. Neuron. 96, 796-807.e6 (2017)

Species mouse

Application WB

Publication Nascimbeni, A. et al. Local detection of PtdIns3P at autophagosome biogenesis membrane platforms. Autophagy. 13, 1602-1612 (2017).

Species human

Application WB

Publication Feldmann, A. et al. The RAB GTPase RAB18 modulates macroautophagy and proteostasis. Biochem. Biophys. Res. Commun. 486, 738–743 (2017).

Species human

Application WB,IHC (frozen)

Publication Yanagisawa, H. et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci.Rep. 7, 15944 (2017).

Species human

Application WB

Publication Fung, G. et al. Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget. 8, 74767-74780 (2017).

Species mouse,rat

Application WB

Publication Mishra, P. et al. Discovery of pan autophagy inhibitors through a high-throughput screen highlights macroautophagy as an evolutionarily conserved process across 3 eukaryotic kingdoms. Autophagy. 13, 1556-1572 (2017).

Species mouse

Application WB,ICC-IF

Publication Kravic, B. et al. In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy. Autophagy. 14, 311-335 (2017)

Species mouse

Application IHC (frozen)

Publication Bartolomeo, R. et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J.Clin.Invest. 127, 3717-3729 (2017)

Species mouse

Application IHC-IF (frozen)

Publication Jacquier, A. et al. Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death. Acta Neuropathol. Commun. 5, 55 (2017).

Species human,chicken

Application IHC (frozen),ICC-IF

Publication Aas, A. et al. Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy. PLoS One 12, e0168182 (2017).

Species mouse

Application WB

Publication Joachim, J. et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr. Biol. 27, 2123–2136.e7 (2017).

Species human

Application ICC-IF

Publication Ruf, S. et al. PLK1 (polo like kinase 1) inhibits MTOR complex 1 and promotes autophagy. Autophagy 13, 486–505 (2017).

Species human

Application ICC-IF

Publication Vargas, T. et al. Selective degradation of PU.1 during autophagy represses the differentiation and antitumour activity of TH9 cells. Nat.Commun. 8, 559 (2017).

Species mouse

Application WB

Publication Kakuta, S. et al. Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation. FASEB.J. 31, 3757-3773 (2017)

Species human

Application ICC-IF

Publication Cui, D. et al. Impaired autophagosome clearance contributes to neuronal death in a piglet model of neonatal hypoxic-ischemic encephalopathy. Cell Death Dis. 8, e2919 (2017)

Species pig

Application ICC-IF

Publication Qiang, L. et al. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis. Autophagy. 13, 2086-2103 (2017)

Species human,mouse

Application WB,IHC (paraffin),ICC-IF

Publication Yanagisawa, H. et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human N324ann-Pick type C disease. Sci.Rep. 7, 15944 (2017).

Species human

Application WB

Publication Mildenberger, J. et al. N-3 PUFAs induce inflammatory tolerance by formation of KEAP1-containing SQSTM1/p62-bodies and activation of NFE2L2. Autophagy 00–00 (2017).

Species mouse

Application WB

Publication Todoric, J. et al. Stress-Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas. Cancer.Cell. 32, 824-839.e8 (2017)

Species mouse

Application IHC (paraffin)

Publication Fajardo, V. A. et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS One 12, e0173708 (2017).

Species mouse

Application WB

Publication Yamanaka, T. et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Nat. Publ. Gr. 6, (2016).

Species mouse

Application WB,IHC (frozen)

Publication Kaizuka, T. & Mizushima, N. Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Mol. Cell. Biol. 36, 585–595 (2016).

Species mouse

Application WB,ICC-IF

Publication Santanam, U. et al. Atg7 cooperates with Pten loss to drive prostate cancer tumor growth. Genes Dev. 30, 399–407 (2016).

Species mouse

Application WB

Publication Angelini, C., Nascimbeni, A. C., Cenacchi, G. & Tasca, E. Lipolysis and lipophagy in lipid storage myopathies. Biochim. Biophys. Acta 1367–1373 (2016).

Species human

Application WB,IHC (frozen)

Publication Saito, T. et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,IHC (paraffin)

Publication Salas, E., Roy, S., Marsh, T., Rubin, B. & Debnath, J. Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene 35, 2913–2922 (2016).

Species mouse

Application ICC-IF

Publication Klionsky, D. J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12, 1 (2016).

Species mouse

Application WB

Publication Starheim, K. et al. Intracellular glutathione determines borte. cytotoxicity in multiple myeloma cells. Blood Cancer J. 6, (2016).

Species human

Application WB

Publication Lim, S. W., Jin, L., Jin, J. & Yang, C. W. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Nat. Publ. Gr. 6, (2016).

Species rat

Application WB,IHC (paraffin)

Publication Ra, E. A. et al. TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells. Nat. Commun. 7, (2016).

Species human

Application WB

Publication Corcelle-Termeau, E. et al. Excess sphingomyelin disturbs ATG9A trafficking and autophagosome closure. Autophagy 12, 533–849 (2016)

Species mouse

Application WB

Publication Duleh, S., Wang, X., Komirenko, A. & Margeta, M. Activation of the Keap1/Nrf2 stress response pathway in autophagic vacuolar myopathies. Acta Neuropathol. Commun. 4, 115 (2016).

Species human,mouse

Application WB,IHC (frozen)

Publication Sergin, I. et al. p62-enriched inclusion bodies in macrophages protect against atherosclerosis HHS Public Access. Sci. Signal. 9, (2016).

Species mouse

Application IHC (frozen),ICC-IF

Publication Achour, I. et al. Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model. Int. J. Mol. Sci. 17, 1293 (2016)

Species rat

Application WB

Publication Puvirajesinghe, T. M. et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,ICC-IF

Publication Baranowska, K. et al. Hydroxychloroquine potentiates carfilzomib toxicity towards myeloma cells. Oncotarget 7, 70845–70856 (2016)

Species human

Application WB

Publication Puvirajesinghe, T. M. et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2–JNK signalling in breast cancer. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,ICC-IF

Publication Venkatesh, A., Ma, S. & Punzo, C. TSC but not PTEN loss in starving cones of retinitis pigmentosa mice leads to an autophagy defect and mTORC1 dissociation from the lysosome. Cell Death Dis. 7, e2279–e2279 (2016).

Species mouse

Application IHC-IF

Publication Zhang, J. et al. A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects. PLoS Genet. 12,(2016).

Species human

Application WB

Publication Taneike, M. et al. mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy. PLoS One 11, (2016).

Species mouse

Application WB

Publication Jarvela, T. S. et al. The neural chaperone proSAAS blocks α-synuclein fibrillation and neurotoxicity. Proc. Natl. Acad. Sci. U. S. A. (2016).

Species human

Application IHC (frozen)

Publication Kenific, C. M. et al. NBR1 enables autophagy-dependent focal adhesion turnover. J. Cell Biol. 212, 577–90 (2016).

Species human

Application WB

Publication Lim, J. et al. Proteotoxic Stress Induces Phosphorylation of p62/SQSTM1 by ULK1 to Regulate Selective Autophagic Clearance of Protein Aggregates. PLoS One 11, (2015).

Species human,mouse

Application WB,ICC-IF

Publication Arel-Dubeau, A.-M. et al. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxid. Med. Cell. Longev. 2014, 15 (2015).

Species rat

Application WB

Publication Hieke, N. et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy 11, 1471–1483 (2015).

Species mouse

Application WB,ICC-IF

Publication Akizu, N. et al. Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction HHS Public Access. Nat. Genet. 47, 528–534 (2015).

Species human

Application WB

Publication Milan, E. et al. A plastic SQSTM1/p62-dependent autophagic reserve maintains proteostasis and determines proteasome inhibitor susceptibility in multiple myeloma cells. Autophagy 11, 1161–1178 (2015).

Species human

Application WB,ICC-IF

Publication Eino, A. et al. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. J. Cell Sci. 128, 4453–61 (2015).

Species mouse

Application IHC (paraffin)

Publication Newton, P. T., Vuppalapati, K. K., Bouderlique, T. & Chagin, A. S. Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner. Autophagy 11, 1594–1607 (2015).

Species mouse,rat

Application WB

Publication Ciuffa, R. et al. The selective autophagy receptor p62 forms a flexible filamentous helical scaffold. Cell Rep. 11, 748–58 (2015).

Species human

Application WB

Publication Pellegrini, C. et al. All-trans retinoic acid and rapamycin normalize Hutchinson Gilford progeria fibroblast phenotype. Oncotarget 6, 29914–29928 (2015).

Species human

Application WB

Publication Johansson, I. et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy 11, 1636–1651 (2015).

Species human,mouse

Application WB,ICC-IF

Publication Lenoir, O. et al. Endothelial cell and podocyte autophagy synergistically protect from diabetes-induced glomerulosclerosis. Autophagy 11, 1130–45 (2015).

Species mouse

Application WB

Publication Clayton, E. L. et al. Frontotemporal dementia caused by CHMP2B mutation is characterised by neuronal lysosomal storage pathology. Acta Neuropathol. 130, 511–23 (2015).

Species mouse

Application IHC (paraffin),IEM

Publication Murrow, L., Malhotra, R. & Debnath, J. ATG12-ATG3 Interacts with Alix to Promote Basal Autophagic Flux and Late Endosome Function. Nat. Cell Biol. 17, 300–310 (2015).

Species mouse

Application WB

Publication Agarwal, S. et al. Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol- 3-phosphate-specific phospholipase A1 activity. Nat. Commun. 6, (2015).

Species human

Application WB

Publication Echeverry, N. et al. Inhibition of autophagy sensitizes malignant pleural mesothelioma cells to dual PI3K/mTOR inhibitors. Cell Death Dis. 6, (2015).

Species human

Application WB

Publication Ulbricht, A. et al. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy 11, 538–546 (2015).

Species human

Application WB

Publication Ruozi, G. et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat. Commun. 6, (2015).

Species mouse

Application WB

Publication Zhou, Z. et al. Autophagy supports color vision. Autophagy 11, 1821–1832 (2015).

Species mouse

Application IHC (frozen)

Publication Liu, S. et al. Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. Cell Death Dis. 6, (2015).

Species rat

Application WB,IHC (frozen)

Publication Chandrachud, U. et al. Unbiased Cell-based Screening in a Neuronal Cell Model of Batten Disease Highlights an Interaction between Ca 2+ Homeostasis, Autophagy, and CLN3 Protein Function. J. Biol. Chem. 290, 14361–14380 (2015).

Species mouse

Application WB

Publication Lodder, J. et al. Macrophage autophagy protects against liver fibrosis in mice. Autophagy 11, 1280–1292 (2015).

Species mouse

Application ICC-IF

Publication Larroquette, F. et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum. Mol. Genet. 24, 6515–6529 (2015).

Species mouse

Application WB

Publication Morimoto, D. et al. The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates. Nat. Commun. 6, (2015).

Species mouse

Application WB,ICC-IF

Publication Felzen, V. et al. Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 6, (2015).

Species human

Application ICC-IF

Publication Shi, J. et al. NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell Death Dis. 6, (2015).

Species human

Application WB

Publication McMillan, E. M. et al. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS One 10, e0119382 (2015).

Species rat

Application WB

Publication Sarkar, C. et al. Impaired autophagy flux is associated with neuronal cell death after traumatic brain injury. Autophagy 10, 2208–2222 (2014).

Species mouse

Application IHC (frozen)

Publication Yamanaka, T. et al. NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat. Commun. 5, (2014).

Species mouse

Application WB,IHC (frozen),IEM

Publication Rivera, J. F., Costes, S., Gurlo, T., Glabe, C. G. & Butler, P. C. Autophagy defends pancreatic β cells from human islet amyloid polypeptide-induced toxicity. J. Clin. Invest. 124, 3489–500 (2014).

Species mouse

Application WB,IHC (paraffin)

Publication Guan, B.-J. et al. Translational Control during Endoplasmic Reticulum Stress beyond Phosphorylation of the Translation Initiation Factor eIF2␣ *. J. Biol. Chem. 289, 12593–12611 (2014).

Species mouse

Application WB

Publication Ost, M., Werner, F., Dokas, J., Klaus, S. & Voigt, A. Activation of AMPKa2 Is Not Crucial for Mitochondrial Uncoupling-Induced Metabolic Effects but Required to Maintain Skeletal Muscle Integrity. PLoS One 9, (2014).

Species mouse

Application WB

Publication Furuya, N. et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631–41 (2014).

Species mouse

Application WB

Publication Kinsey, C. et al. Plac8 links oncogenic mutations to regulation of autophagy and is critical to pancreatic cancer progression. Cell Rep. 7, 1143–1155 (2014).

Species human,mouse

Application WB

Publication Lim, Y.-M. et al. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes. Nat. Commun. 5, (2014).

Species mouse

Application WB

Publication Heijnen, H. F. et al. Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway. PLoS Genet. 10, (2014).

Species human

Application WB,ICC-IF

Publication Nicot, A.-S. et al. Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy 10, 1036–53 (2014).

Species human

Application WB

Publication Cottam, E. M., Whelband, M. C. & Wileman, T. Coronavirus NSP6 restricts autophagosome expansion. Autophagy 10, (2014).

Species monkey

Application WB,ICC-IF

Publication Dunlop, E. A. et al. FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation. Autophagy 10, 1749–1760 (2014).

Species human,mouse

Application WB,IHC

Publication Costes, S., Gurlo, T., Rivera, J. F. & Butler, P. C. UCHL1 deficiency exacerbates human islet amyloid polypeptide toxicity in β-cells: evidence of interplay between the ubiquitin/proteasome system and autophagy. Autophagy 10, 1004–14 (2014).

Species mouse

Application WB,IHC (paraffin)

Publication Karlsson, A. B. et al. The role of spartin and its novel ubiquitin binding region in DALIS occurrence. Mol. Biol. Cell 25, 1355–1365 (2014).

Species mouse

Application ICC-IF

Publication Xu, X. et al. Autophagy is essential for effector CD8 T cell survival and memory formation. Nat. Immunol. 15, 1152–1161 (2014).

Species mouse

Application WB

Publication Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

Species mouse

Application IHC (frozen)

Publication Kondratskyi, A. et al. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death Dis. 5, (2014).

Species human

Application WB

Publication Gabandé-Rodrıuez, E., Boya, P., Labrador, V., Dotti, C. & Ledesma, M. High sphingomyelin levels induce lysosomal damage and autophagy dysfunction in N324ann Pick disease type A. Cell Death Differ. 21, 864–875 (2014).

Species mouse

Application WB

Publication Sahani, M. H., Itakura, E. & Mizushima, N. Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids. Autophagy 10, 431–41 (2014).

Species mouse

Application WB,ICC-IF

Publication Kageyama, S. et al. Proteasome Dysfunction Activates Autophagy and the Keap1-Nrf2 Pathway. J. Biol. Chem. 289, 24944–24955 (2014).

Species mouse

Application WB,IHC (frozen)

Publication Khan, M. M. et al. Role of autophagy, SQSTM1, SH3GLB1, and TRIM63 in the turnover of nicotinic acetylcholine receptors. Autophagy 10, 123–36 (2014).

Species mouse

Application WB,IHC

Publication Tanaka, K. et al. A Novel Acylaminoimidazole Derivative, WN1316, Alleviates Disease Progression via Suppression of Glial Inflammation in ALS Mouse Model. PLoS One 9, (2014).

Species mouse

Application WB

Publication Ricketts, T. et al. A nonsense mutation in mouse Tardbp affects TDP43 alternative splicing activity and causes limb-clasping and body tone defects. PLoS One 9, (2014).

Species mouse

Application IHC (paraffin)

Publication Zhang, L. et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat. Commun. 5, (2014).

Species mouse

Application WB,IHC (paraffin),ICC-IF,IP

Publication Yamano, K. & Youle, R. J. PINK1 is degraded through the N-end rule pathway. Autophagy 9, 1758–69 (2013).

Species human

Application ICC-IF

Publication Ozcelik, S. et al. Rapamycin Attenuates the Progression of Tau Pathology in P301S Tau Transgenic Mice. PLoS One 8, (2013)

Species mouse

Application WB

Publication Jiang, S., Guo, R., Zhang, Y., Zou, Y. & Ren, J. Heavy metal scavenger metallothionein mitigates deep hypothermia-induced myocardial contractile anomalies: role of autophagy. Am. J. Physiol. - Endocrinol. Metab. 304, (2013).

Species mouse

Application WB

Publication Kondylis, V. et al. Endosome-mediated autophagy: an unconventional MIIC-driven autophagic pathway operational in dendritic cells. Autophagy 9, 861–80 (2013).

Species mouse

Application WB,ICC-IF,IEM

Publication Schober, A. et al. Cell Loss and Autophagy in the Extra‐Adrenal Chromaffin Organ of Zuckerkandl are Regulated by Glucocorticoid Signalling. J. Neuroendocrinol. 25, 34–47 (2013)

Species mouse

Application ICC-IF

Publication Yuan, H.-X., Russell, R. C. & Guan, K.-L. Regulation of PIK3C3/VPS34 complexes by MTOR in nutrient stress-induced autophagy. Autophagy 9, 1983–95 (2013).

Species human,mouse

Application WB

Publication Shackelford, D. B. et al. LKB1 inactivation dictates therapeutic response of non-small cell lung cancer to the metabolism drug phenformin. Cancer Cell 23, 143–58 (2013).

Species human

Application WB,IHC

Publication Spilman, P. et al. Inhibition of mTOR by Rapamycin Abolishes Cognitive Deficits and Reduces Amyloid-β Levels in a Mouse Model of Alzheimer’s Disease. PLoS One 5, e9979 (2010).

Species mouse

Application WB

Publication Waguri, S. & Komatsu, M. Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice. Methods in Enzymology 453, 181–196 (2009).

Species mouse

Application WB,IHC (frozen)

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File IHC guinea pig antibody

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Category Protocol

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Datasheet

MSDS

FAQs

PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
Use the antibody in a higher dilution, but prolong incubation time and exposure time.
Always use a fresh aliquot of the antibody.
Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
Include an additional washing step.
You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
Always use a fresh aliquot of secondary antibody.
In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

The concentration of specific antibody in our guinea pig serum is not determined.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.

Progen Support

For scientific and customer support, you can reach us by phone or email.