Highly published

anti-p62/ SQSTM1 (C-terminus) guinea pig polyclonal, serum

Cat. No: GP62-C

Prices excl. tax

Available, delivery time 1-7 days

$343.00*

Datasheet

Key Features

Guinea pig polyclonal
Suitable for IHC and WB
Reacts with bovine, human, mouse and rat

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	IHC, WB
Purification	Stabilized antiserum
Reactivity	Bovine, Human, Mouse, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	C-terminal domain (20 amino acids: C-NYD IGA ALD TIQ YSK HPP PL) of human p62 protein, coupled to KLH. This peptide sequence is identical in human, monkey, bovine, mouse, and rat.
Formulation	Contains 0.09% sodium azide
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:100-1:600
Immunohistochemistry (IHC) - paraffin	1:100-1:600 (microwave treatment recommended)
Western Blot (WB)	1:1,000-1:3,000

Background

Human 62 kDa (p62) protein, is present in intracytoplasmic inclusions (e.g. hyaline bodies) of hepatocellular carcinoma.
p62 protein (also described as ubiqutin-binding protein; sequestosome 1; SQSTM1) has been found in many tissues and cells, including lymphoid cells, serving probably a common cellular signal transduction mechanism (e.g. ubiquitin-associated degradation and autophagy).
The antiserum stains also neurofibrillary tangles in the brain of patients suffering from Alzheimer's disease.

The GP62-C antibody is knockout validated (Waguri & Komatsu, 2009).

Waguri, S. & Komatsu, M. Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice. Methods in Enzymology 453, 181-196 (2009).

References/Publications (253)

Publication

Species

Application

Publication Li, Y. et al. Impairment of autophagy after spinal cord injury potentiates neuroinflammation and motor function deficit in mice., Theranostics 12, 5364-5388, (2022).

Species mouse

Application IHC

Publication Xiaojing Kang Shin Shimada Hiroki Miyahara Keiichi Higuchi Masayuki Mori. BALB.NCT-Cpox nct is a unique mouse model of hereditary coproporphyria., Mol Genet Metab Rep, 35100964, (2023).

Species mouse

Application IHC/IF

Publication Iannibelli E. et al., VCP-related myopathy: a case series and a review of literature., Acta Myol, 42, 2-13, (2023).

Species Human

Application IHC-IF

Publication Akwa Y. et al., Stimulation of synaptic activity promotes TFEB-mediated clearance of pathological MAPT/Tau in cellular and mouse models of tauopathies., Autophagy, 19, 660-677, (2023).

Species Mouse

Application IHC

Publication Opland C. K. et al. Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity., iScience, 26, 106905, (2023).

Species human, mouse

Application WB

Publication Kataura, T. et al. NDP52 acts as a redox sensor in PINK1/Parkin‐mediated mitophagy. EMBO J. e111372 (2022).

Species human

Application WB

Publication De Mazière A. et al. An optimized protocol for immuno-electron microscopy of endogenous LC3., Autophagy, 18, 3004-3022, (2022).

Species Human

Application EM

Publication Varea, O., Guinovart, J. J. & Duran, J. Malin restoration as proof of concept for gene therapy for Lafora disease. Brain Commun. 4, (2022).

Species mouse

Application IHC-IF (paraffin)

Publication Suzuki, C., Yamaguchi, J., et al. Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins. Sci Rep. (2022).

Species Mouse

Application IHC

Publication Schmitt D. et al. Lipid and protein content profiling of isolated native autophagic vesicles., EMBO Rep, 23, e53065, (2022).

Species human

Application ICC, WB

Publication Aber E. R. et al., Oligodendroglial macroautophagy is essential for myelin sheath turnover to prevent neurodegeneration and death., Cell Rep, 41, 111480, (2022).

Species Mouse

Application IHC-IF

Publication Jackson, J. et al. SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells. Mol. Metab. 61, 101503 (2022).

Species Human

Application WB

Publication Gozzelino L. et al., Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy., Brain, 145, 2313-2331, (2022).

Species Mouse

Application IHC-IF

Publication Abdullah C. S. et al. Mitochondrial dysfunction and autophagy activation are associated with cardiomyopathy developed by extended methamphetamine self-administration in rats., Redox Biol, 58, 102523, (2022).

Species rat

Application WB

Publication Sharma, K. et al. Autophagy modulates cell fate decisions during lineage commitment., Autophagy 18, 1915-1931, (2022).

Species human

Application WB

Publication Yamada, T. et al. Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver. iScience 25, 103996 (2022).

Species Mouse

Application IHC-Fr-IF

Publication Solvik, T. A. et al. Secretory autophagy maintains proteostasis upon lysosome inhibition., J Cell Biol 221, (2022).

Species human

Application ICC-IF, WB

Publication Kiryu-Seo, S. et al. Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons., EMBO J 41, e110486, (2022)

Species mouse

Application IHC-IF

Publication Sønder, S.L. et al. Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis. Sci Adv. 7, NULL(2021).

Species human

Application WB

Publication Schlütermann, D. et al. FIP200 controls the TBK1 activation threshold at SQSTM1/p62-positive condensates. Sci. Rep. 11, (2021).

Species mouse

Application WB

Publication Mercer, T. J. et al. Phosphoproteomic identification of ULK substrates reveals VPS15‐dependent ULK/VPS34 interplay in the regulation of autophagy. EMBO J. 40, (2021).

Species human

Application ICC-IF, WB

Publication Goodwin, J.M. et al. GABARAP sequesters the FLCN-FNIP tumor suppressor complex to couple autophagy with lysosomal biogenesis. Sci Adv. 7, eabj2485(2021).

Species human

Application WB

Publication Deitersen, J. et al. High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol. Cell Death Dis. 12, (2021).

Species human

Application WB

Publication Sargent, D. et al. Neuronal VPS35 deletion induces spinal cord motor neuron degeneration and early post-natal lethality . Brain Commun. 3, 1–20 (2021).

Species Mouse

Application IHC-IF

Publication Nthiga, T. M. et al. Regulation of golgi turnover by CALCOCO1-mediated selective autophagy. J. Cell Biol. 220, (2021).

Species Human

Application WB

Publication Kawakami, I. et al. Progression of phosphorylated α-synuclein in Macaca fuscata. Brain Pathol. 31, e12952(2021).

Species macaque

Application IHC (paraffin)

Publication Rudnick, J. A. et al. Autophagy in stromal fibroblasts promotes tumor desmoplasia and mammary tumorigenesis. Genes Dev. 35, 963–975 (2021).

Species Mouse

Application IHC-P

Publication Nozaki, M. et al. SQSTM1L341V variant that is linked to sporadic ALS exhibits impaired association with MAP1LC3 in cultured cells. eNeurologicalSci. 22, 100301(2021).

Species mouse

Application ICC/IF

Publication Hegdekar, N., Lipinski, M. M. & Sarkar, C. N-Acetyl-l-leucine improves functional recovery and attenuates cortical cell death and neuroinflammation after traumatic brain injury in mice. Sci. Rep. 11, (2021).

Species mouse

Application IHC-IF

Publication Cui, Y. H. et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat. Commun. 12, 1–19 (2021).

Species human

Application WB

Publication Schmitz, K. et al. An essential role of the autophagy activating kinase ULK1 in snRNP biogenesis. Nucleic Acids Res. 49, 6437–6455 (2021).

Species Human

Application WB

Publication Keller, F. et al. A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells 9, (2020).

Species human

Application ICC

Publication Fucci, C. et al. The Interaction of the Tumor Suppressor FAM46C with p62 and FNDC3 Proteins Integrates Protein and Secretory Homeostasis. Cell Rep. 32, 108162(2020).

Species human

Application ICC/IF

Publication Aishwarya, R. et al. Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytes. Redox Biol. 36, 101660(2020).

Species rat

Application WB

Publication Bhushan, J. et al. Isg15 connects autophagy and ifn-dependent control of toxoplasma gondii infection in human cells. MBio 11, 1–19 (2020).

Species human

Application ICC-IF, WB

Publication Kakimoto, Y. et al. Myocardial cathepsin D is downregulated in sudden cardiac death. PLoS.One. 15, e0230375 (2020)

Species human

Application WB

Publication Manzetti, J. et al. BK Polyomavirus Evades Innate Immune Sensing by Disrupting the Mitochondrial Network and Promotes Mitophagy. iScience. 23, 101257(2020).

Species human

Application IHC (paraffin)/IF

Publication Jakobi, A. et al. Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake. Nat.Commun. 11, 440 (2020)

Species human

Application WB

Publication Takahashi, S. et al. Loss of autophagy impairs physiological steatosis by accumulation of NCoR1. Life.Sci.Alliance. 3, (2020)

Species mouse

Application WB

Publication Kurashige, T. et al. Hormonal Regulation of Autophagy in Thyroid PCCL3 Cells and the Thyroids of Male Mice. J.Endocr.Soc. 4, bvaa054 (2020)

Species rat

Application WB,IHC-IF (paraffin)

Publication Noman, M. et al. Inhibition of Vps34 reprograms cold into hot inflamed tumors and improves anti-PD-1/PD-L1 immunotherapy. Sci.Adv. 6, eaax7881 (2020)

Species mouse

Application WB

Publication Baeken, M. W. et al. Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Cells 9, (2020).

Species human

Application ICC-IF, WB

Publication Werner, G. et al. Loss of TMEM106B potentiates lysosomal and FTLD-like pathology in progranulin-deficient mice. EMBO Rep. 21, e50241(2020).

Species mouse

Application IHC/IF

Publication Dohmen, M. et al. AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nat.Commun. 11, 1032 (2020)

Species mouse

Application WB

Publication Metti, S. et al. The Polyphenol Pterostilbene Ameliorates the Myopathic Phenotype of Collagen VI Deficient Mice via Autophagy Induction. Front. Cell Dev. Biol. 8, (2020).

Species mouse

Application IHC-IF

Publication Saegusa, C. et al. Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells.. Laryngoscope Investig Otolaryngol. 5, 520-528(2020).

Species mouse

Application IHC/IF

Publication Choi, I. et al. Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration. Nat.Commun. 11, 1386 (2020)

Species mouse

Application ICC-IF

Publication Leidal, A. et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat.Cell.Biol. 22, 187-199 (2020)

Species human

Application WB

Publication Blázquez, C. et al. Inhibition of striatonigral autophagy as a link between cannabinoid intoxication and impairment of motor coordination. Elife 9, 1–24 (2020).

Species mouse

Application WB

Publication Dubois, C. et al. Co-targeting Mitochondrial Ca2+ Homeostasis and Autophagy Enhances Cancer Cells' Chemosensitivity. iScience. 23, 101263(2020).

Species human

Application WB

Publication Deng, Z. et al. ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway. Autophagy. 16, 917-931 (2020)

Species human,mouse

Application WB

Publication Sánchez-López, E. et al. Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production. Cell.Metab. 29, 1350-1362.e7 (2019)

Species mouse

Application ICC

Publication Ma, X. et al. Transcription Factor EB Activation Rescues Advanced αB-Crystallin Mutation-Induced Cardiomyopathy by Normalizing Desmin Localization. J.Am.Heart.Assoc. 8, e010866 (2019)

Species mouse

Application IHC (paraffin)

Publication Pellegrini, C. et al. Altered adipocyte differentiation and unbalanced autophagy in type 2 Familial Partial Lipodystrophy: an in vitro and in vivo study of adipose tissue browning. Exp.Mol.Med. 51, 89 (2019)

Species human

Application WB,ICC-IF

Publication Carballo-Carbajal, I. et al. Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis. Nat.Commun. 10, 973 (2019)

Species rat

Application WB,IHC (paraffin)

Publication Lachance, V. et al. Autophagy protein NRBF2 has reduced expression in Alzheimer's brains and modulates memory and amyloid-beta homeostasis in mice. Mol.Neurodegener. 14, 43 (2019)

Species mouse

Application WB

Publication Birgisdottir, Å. et al. Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs. Autophagy. , Jan-23 (2019)

Species human

Application WB,IHC,IP

Publication Igawa, H. et al. p62-mediated autophagy affects nutrition-dependent insulin receptor substrate 1 dynamics in 3T3-L1 preadipocytes. J.Diabetes.Investig. 10, 32-42 (2019)

Species mouse

Application WB

Publication Riemschoss, K. et al. Fibril-induced glutamine-/asparagine-rich prions recruit stress granule proteins in mammalian cells. Life.Sci.Alliance. 2, (2019)

Species mouse

Application WB,ICC-IF

Publication Overå, K. et al. TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1. J.Cell.Sci. 132, (2019)

Species human

Application WB

Publication Jung, S. et al. Buffering of cytosolic calcium plays a neuroprotective role by preserving the autophagy-lysosome pathway during MPP+-induced neuronal death. Cell.Death.Discov. 5, 130 (2019)

Species mouse

Application WB

Publication Anisimov, S. et al. G3BP1 inhibits ubiquitinated protein aggregations induced by p62 and USP10. Sci.Rep. 9, 12896 (2019)

Species human

Application WB

Publication Liang, S. et al. Murine macrophage autophagy protects against alcohol-induced liver injury by degrading interferon regulatory factor 1 (IRF1) and removing damaged mitochondria. J.Biol.Chem. , (2019)

Species mouse

Application WB

Publication Tang, T. et al. Dihydroartemisinin and its anticancer activity against endometrial carcinoma and cervical cancer: involvement of apoptosis, autophagy and transferrin receptor. Singapore.Med.J. , (2019)

Species human

Application WB

Publication Himuro, M. et al. Cellular Autophagy in α Cells Plays a Role in the Maintenance of Islet Architecture. J.Endocr.Soc. 3, 1979-1992 (2019)

Species mouse

Application IHC (paraffin)

Publication Tamura, N. et al. Hyperosmotic stress induces unconventional autophagy independent of the Ulk1 complex. Mol.Cell.Biol. , (2019)

Species human,mouse

Application WB,ICC

Publication Kim, W. et al. Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia. Sci.Rep. 9, 3021 (2019)

Species mouse

Application IHC (paraffin)

Publication Chakraborty, D. et al. Enhanced autophagic-lysosomal activity and increased BAG3-mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress. Redox.Biol. 24, 101181 (2019)

Species mouse

Application WB

Publication Vanzo, R. et al. Autophagy role(s) in response to oncogenes and DNA replication stress. Cell.Death.Differ. , (2019)

Species human

Application WB

Publication Glatigny, M. et al. Autophagy Is Required for Memory Formation and Reverses Age-Related Memory Decline. Curr.Biol. 29, 435-448.e8 (2019)

Species mouse

Application WB,IHC-IF (frozen)

Publication Yang, S. et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nat.Commun. 10, 2782 (2019)

Species human

Application WB

Publication Wang, Y. et al. Autophagy inhibition specifically promotes epithelial-mesenchymal transition and invasion in RAS-mutated cancer cells. Autophagy. , Jan-14 (2019)

Species mouse

Application ICC-IF,WB

Publication Klimek, C. et al. The Hippo network kinase STK38 contributes to protein homeostasis by inhibiting BAG3-mediated autophagy. Biochim. Biophys. Acta - Mol. Cell Res. 1866, 1556–1566 (2019).

Species human

Application WB

Publication Castets, P. et al. mTORC1 and PKB/Akt control the muscle response to denervation by regulating autophagy and HDAC4. Nat.Commun. 10, 3187 (2019)

Species mouse

Application IHC (paraffin)

Publication Pigna, E. et al. Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosis. EBioMedicine. 40, 717-732 (2019)

Species mouse

Application IHC-IF (frozen)

Publication Park, S. et al. A lysosomal chloride ion-selective fluorescent probe for biological applications. Chem.Sci. 10, 56-66 (2019)

Species human

Application ICC-IF

Publication Gurlo, T. et al. Pregnancy in human IAPP transgenic mice recapitulates beta cell stress in type 2 diabetes. Diabetologia. 62, 1000-1010 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Garcia, D. et al. Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell.Rep. 26, 192-208.e6 (2019)

Species mouse

Application WB

Publication Sarkar, C. et al. PLA2G4A/cPLA2-mediated lysosomal membrane damage leads to inhibition of autophagy and neurodegeneration after brain trauma. Autophagy. , 1-20 (2019)

Species mouse

Application ICC

Publication Yang, Y. et al. Cytoplasmic DAXX drives SQSTM1/p62 phase condensation to activate Nrf2-mediated stress response. Nat.Commun. 10, 3759 (2019)

Species human

Application WB

Publication Sarraf, S. et al. PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Cell.Rep. 29, 225-235.e5 (2019)

Species mouse

Application WB

Publication Hoem, G. et al. The FMRpolyGlycine Protein Mediates Aggregate Formation and Toxicity Independent of the CGG mRNA Hairpin in a Cellular Model for FXTAS. Front.Genet. 10, 249 (2019)

Species human

Application ICC-IF

Publication Kinsey, C. et al. Protective autophagy elicited by RAF→MEK→ERK inhibition suggests a treatment strategy for RAS-driven cancers. Nat.Med. 25, 620-627 (2019)

Species mouse

Application IHC (paraffin)

Publication Hill, C. et al. Autophagy inhibition-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in pulmonary fibrosis. Cell.Death.Dis. 10, 591 (2019)

Species human

Application IHC (paraffin)

Publication Aubert, P. et al. Maternal protein restriction induces gastrointestinal dysfunction and enteric nervous system remodeling in rat offspring. FASEB.J. 33, 770-781 (2019)

Species rat

Application WB

Publication Horigome, Y. et al. Loss of autophagy in chondrocytes causes severe growth retardation. Autophagy. , 1-11 (2019)

Species mouse

Application IHC (paraffin)

Publication Sánchez-López, E. et al. Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production. Cell.Metab. 29, 1350-1362.e7 (2019)

Species mouse

Application ICC

Publication Rienzo, M. et al. Autophagy induction in atrophic muscle cells requires ULK1 activation by TRIM32 through unanchored K63-linked polyubiquitin chains. Sci.Adv. 5, eaau8857 (2019)

Species mouse

Application IHC-IF (paraffin)

Publication Aghajan, M. et al. Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice. JCI.Insight. 4, (2019)

Species mouse

Application WB

Publication Sabino, C. et al. Bafilomycin A1 and U18666A Efficiently Impair ZIKV Infection. Viruses. 11, (2019)

Species human

Application WB

Publication Kessler, S. et al. Diethylnitrosamine (DENA) recapitulates formation of hepatic angiosarcoma in pigs. PLoS.One. 14, e0214756 (2019)

Species pig

Application IHC (paraffin)

Publication Zhang, J. et al. Cross-regulation of defective endolysosome trafficking and enhanced autophagy through TFEB in UNC13D deficiency. Autophagy. , 1-19 (2019)

Species mouse

Application WB

Publication Li, Y. et al. cPLA2 activation contributes to lysosomal defects leading to impairment of autophagy after spinal cord injury. Cell.Death.Dis. 10, 531 (2019)

Species mouse

Application IHC (frozen)

Publication Bonsi, P. et al. RGS9-2 rescues dopamine D2 receptor levels and signaling in DYT1 dystonia mouse models. EMBO.Mol.Med. 11, (2019)

Species mouse

Application WB

Publication Gasset-Rosa, F. et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. Neuron. 102, 339-357.e7 (2019)

Species human

Application ICC-IF

Publication Suzuki, C. et al. Autophagy Deficiency in Renal Proximal Tubular Cells Leads to an Increase in Cellular Injury and Apoptosis under Normal Fed Conditions. Int.J.Mol.Sci. 21, (2019)

Species mouse

Application ICC-IF

Publication Lim, J. et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. Elife. 8, (2019)

Species mouse

Application WB

Publication Gorgulu, K. et al. Levels of the Autophagy-Related 5 Protein Affect Progression and Metastasis of Pancreatic Tumors in Mice. Gastroenterology. 156, 203-217.e20 (2019)

Species mouse

Application WB

Publication Olivé, M. et al. Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions. Nat.Commun. 10, 1396 (2019)

Species human

Application IHC (frozen)

Publication Javaheri, A. et al. TFEB activation in macrophages attenuates postmyocardial infarction ventricular dysfunction independently of ATG5-mediated autophagy. JCI.Insight. 4, (2019)

Species human

Application IHC (paraffin)

Publication Nam, S. et al. Autophagy attenuates tubulointerstital fibrosis through regulating transforming growth factor-β and NLRP3 inflammasome signaling pathway. Cell.Death.Dis. 10, 78 (2019)

Species mouse

Application WB,IHC (paraffin)

Publication Pasquier, A. et al. Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes. Nat.Commun. 10, 3312 (2019)

Species mouse

Application ICC-IF

Publication Schwob, A. et al. SQSTM-1/p62 potentiates HTLV-1 Tax-mediated NF-κB activation through its ubiquitin binding function. Sci.Rep. 9, 16014 (2019)

Species human

Application IF,WB

Publication Saito, T. et al. Autophagy regulates lipid metabolism through selective turnover of NCoR1. Nat.Commun. 10, 1567 (2019)

Species mouse

Application WB

Publication Vincent, A. et al. Subcellular origin of mitochondrial DNA deletions in human skeletal muscle. Ann.Neurol. 84, 289-301 (2018).

Species human

Application IHC-IF (frozen)

Publication Osonoi, Y. et al. Defective autophagy in vascular smooth muscle cells enhances cell death and atherosclerosis. Autophagy. 14, 1991-2006 (2018)

Species mouse

Application IHC (frozen)

Publication Mitsui, S. et al. Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1H46R-expressing ALS mouse model. Mol.Brain. 11, 30 (2018).

Species mouse

Application IHC-IF (paraffin)

Publication Kuno, A. et al. Resveratrol Ameliorates Mitophagy Disturbance and Improves Cardiac Pathophysiology of Dystrophin-deficient mdx Mice. Sci.Rep. 8, 15555 (2018).

Species mouse

Application WB

Publication Mauthe, M. et al. Chloroquine inhibits autophagic flux by decreasing autophagosome-lysosome fusion. Autophagy. 14, 1435-1455 (2018)

Species human

Application ICC-IF

Publication Ogmundsdóttir, M. et al. A short isoform of ATG7 fails to lipidate LC3/GABARAP. Sci.Rep. 8, 14391 (2018).

Species mouse

Application WB

Publication Koyuncu, S. et al. The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. Nat.Commun. 9, 2886 (2018).

Species human

Application WB

Publication Lim, H. et al. A novel autophagy enhancer as a therapeutic agent against metabolic syndrome and diabetes. Nat.Commun. 9, 1438 (2018).

Species human

Application WB

Publication Sato, S. et al. Loss of autophagy in dopaminergic neurons causes Lewy pathology and motor dysfunction in aged mice. Sci.Rep. 8, 2813 (2018)

Species mouse

Application WB,IHC-IF (frozen)

Publication Furuya, N. et al. NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy. EMBO.Rep. 19, (2018)

Species human

Application WB

Publication Carroll, B. et al. Oxidation of SQSTM1/p62 mediates the link between redox state and protein homeostasis. Nat.Commun. 9, 256 (2018).

Species human,mouse

Application WB

Publication Kivinen, N. The role of autophagy in age-related macular degeneration. Acta.Ophthalmol. 96 Suppl A110, Jan-50 (2018)

Species mouse

Application IHC (paraffin)

Publication Alam, S. et al. Aberrant Mitochondrial Fission Is Maladaptive in Desmin Mutation-Induced Cardiac Proteotoxicity. J.Am.Heart.Assoc. 7, (2018).

Species mouse

Application WB

Publication Yamada, T. et al. Mitochondrial Stasis Reveals p62-Mediated Ubiquitination in Parkin-Independent Mitophagy and Mitigates Nonalcoholic Fatty Liver Disease. Cell.Metab. , (2018)

Species mouse

Application IHC-IF (paraffin)

Publication Oehl, K. et al. Live-Cell Mesothelioma Biobank to Explore Mechanisms of Tumor Progression. Front.Oncol. 8, 40 (2018).

Species human

Application WB

Publication Nikam, A. et al. The PPARα Agonist Fenofibrate Prevents Formation of Protein Aggregates (Mallory-Denk bodies) in a Murine Model of Steatohepatitis-like Hepatotoxicity. Sci.Rep. 8, 12964 (2018).

Species mouse

Application WB

Publication Miret-Casals, L. et al. Identification of New Activators of Mitochondrial Fusion Reveals a Link between Mitochondrial Morphology and Pyrimidine Metabolism. Cell.Chem.Biol. 25, 268-278.e4 (2018)

Species human

Application WB

Publication Battista, R. et al. Autophagy mediates epithelial cancer chemoresistance by reducing p62/SQSTM1 accumulation. PLoS.One. 13, e0201621 (2018).

Species human

Application WB,ICC-IF

Publication Wu, Z. et al. Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy. Cell.Metab. 28, 130-144.e7 (2018)

Species drosophila

Application WB

Publication McLelland, G. et al. Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy. Elife. 7, (2018).

Species human

Application WB

Publication Takahashi, M. et al. USP10 Is a Driver of Ubiquitinated Protein Aggregation and Aggresome Formation to Inhibit Apoptosis. iScience. 9, 433-450 (2018).

Species human

Application WB,ICC-IF

Publication Ziegler, P. et al. Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. Cell. 174, 88-101.e16 (2018)

Species human

Application IHC-IF (paraffin)

Publication Liu, S. et al. Lysosomal damage after spinal cord injury causes accumulation of RIPK1 and RIPK3 proteins and potentiation of necroptosis. Cell.Death.Dis. 9, 476 (2018).

Species mouse

Application IHC (frozen)

Publication Livingston, J. et al. Analysis of HSP27 and the Autophagy Marker LC3B+ Puncta Following Preoperative Chemotherapy Identifies High-Risk Osteosarcoma Patients. Mol.Cancer.Ther. 17, 1315-1323 (2018)

Species human

Application IHC (paraffin)

Publication Fernández, A. et al. Disruption of the beclin 1-BCL2 autophagy regulatory complex promotes longevity in mice. Nature. 558, 136-140 (2018)

Species mouse

Application WB

Publication Viana-Huete, V. et al. Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology. 159, 323-340 (2018)

Species mouse

Application ICC-IF

Publication Aron, R. et al. Deubiquitinase Usp12 functions noncatalytically to induce autophagy and confer neuroprotection in models of Huntington's disease. Nat.Commun. 9, 3191 (2018).

Species rat

Application WB

Publication Sebastiani, A. et al. Sequestosome 1 Deficiency Delays, but Does Not Prevent Brain Damage Formation Following Acute Brain Injury in Adult Mice. Front.Neurosci. 11, 678 (2018).

Species mouse

Application WB

Publication Matta, S. et al. NADPH Oxidase and Guanylate Binding Protein 5 Restrict Survival of Avirulent Type III Strains of Toxoplasma gondii in Naive Macrophages. MBio. 9, (2018).

Species mouse

Application ICC-IF

Publication Barutcu, S. et al. Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy. Autophagy. 14, 1586-1595 (2018).

Species mouse

Application WB

Publication Prabakaran, T. et al. Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1. EMBO.J. 37, (2018)

Species human,mouse

Application WB,ICC-IF

Publication Kageyama, S. et al. Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Mol.Cell.Biol. , (2018).

Species mouse

Application WB

Publication Nwadike, C. et al. AMPK Inhibits ULK1-Dependent Autophagosome Formation and Lysosomal Acidification via Distinct Mechanisms. Mol.Cell.Biol. 38, (2018).

Species mouse

Application ICC

Publication Sebori, R. et al. Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice. Oxid.Med.Cell.Longev. 2018, 9179270 (2018).

Species mouse

Application WB

Publication Mejlvang, J. et al. Starvation induces rapid degradation of selective autophagy receptors by endosomal microautophagy. J.Cell.Biol. 217, 3640-3655 (2018)

Species human

Application WB,ICC-IF

Publication Cabe, M. et al. PB1 and UBA domains of p62 are essential for aggresome-like induced structure formation. Biochem.Biophys.Res.Commun. 503, 2306-2311 (2018)

Species mouse

Application WB

Publication Kakuta, S. et al. Small GTPase Rab1B is associated with ATG9A vesicles and regulates autophagosome formation. FASEB.J. 31, 3757-3773 (2017)

Species human

Application ICC-IF

Publication Qiang, L. et al. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis. Autophagy. 13, 2086-2103 (2017)

Species human,mouse

Application WB,IHC (paraffin),ICC-IF

Publication Mishra, P. et al. Discovery of pan autophagy inhibitors through a high-throughput screen highlights macroautophagy as an evolutionarily conserved process across 3 eukaryotic kingdoms. Autophagy. 13, 1556-1572 (2017).

Species mouse

Application WB,ICC-IF

Publication Cui, D. et al. Impaired autophagosome clearance contributes to neuronal death in a piglet model of neonatal hypoxic-ischemic encephalopathy. Cell Death Dis. 8, e2919 (2017)

Species pig

Application ICC-IF

Publication Yanagisawa, H. et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human N324ann-Pick type C disease. Sci.Rep. 7, 15944 (2017).

Species human

Application WB

Publication Mildenberger, J. et al. N-3 PUFAs induce inflammatory tolerance by formation of KEAP1-containing SQSTM1/p62-bodies and activation of NFE2L2. Autophagy 00–00 (2017).

Species mouse

Application WB

Publication Vargas, T. et al. Selective degradation of PU.1 during autophagy represses the differentiation and antitumour activity of TH9 cells. Nat.Commun. 8, 559 (2017).

Species mouse

Application WB

Publication Kravic, B. et al. In mammalian skeletal muscle, phosphorylation of TOMM22 by protein kinase CSNK2/CK2 controls mitophagy. Autophagy. 14, 311-335 (2017)

Species mouse

Application IHC (frozen)

Publication Fajardo, V. A. et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS One 12, e0173708 (2017).

Species mouse

Application WB

Publication Todoric, J. et al. Stress-Activated NRF2-MDM2 Cascade Controls Neoplastic Progression in Pancreas. Cancer.Cell. 32, 824-839.e8 (2017)

Species mouse

Application IHC (paraffin)

Publication Bartolomeo, R. et al. mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J.Clin.Invest. 127, 3717-3729 (2017)

Species mouse

Application IHC-IF (frozen)

Publication Ruf, S. et al. PLK1 (polo like kinase 1) inhibits MTOR complex 1 and promotes autophagy. Autophagy 13, 486–505 (2017).

Species human

Application ICC-IF

Publication Joachim, J. et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr. Biol. 27, 2123–2136.e7 (2017).

Species human

Application ICC-IF

Publication Aas, A. et al. Nucleocytoplasmic Shuttling of FTO Does Not Affect Starvation-Induced Autophagy. PLoS One 12, e0168182 (2017).

Species mouse

Application WB

Publication Jacquier, A. et al. Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death. Acta Neuropathol. Commun. 5, 55 (2017).

Species human,chicken

Application IHC (frozen),ICC-IF

Publication Fung, G. et al. Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget. 8, 74767-74780 (2017).

Species mouse,rat

Application WB

Publication McLendon, P. et al. An Unbiased High-Throughput Screen to Identify Novel Effectors That Impact on Cardiomyocyte Aggregate Levels. Circ.Res. 121, 604-616 (2017).

Species rat

Application WB

Publication Shiba-Fukushima, K. et al. Evidence that phosphorylated ubiquitin signaling is involved in the etiology of Parkinson's disease. Hum.Mol.Genet. 26, 3172-3185 (2017)

Species human

Application WB

Publication Lee, M. Y. et al. Peroxisomal protein PEX13 functions in selective autophagy. EMBO Rep. 18, 48–60 (2017).

Species mouse

Application WB

Publication Wallot-Hieke, N. et al. Systematic analysis of ATG13 domain requirements for autophagy induction. Autophagy. , 1-58 (2017)

Species mouse

Application ICC-IF

Publication Hartupee, J. et al. Impaired Protein Quality Control During Left Ventricular Remodeling in Mice With Cardiac Restricted Overexpression of Tumor Necrosis Factor. Circ.Heart.Fail. 10, (2017).

Species mouse

Application IHC-IF (paraffin)

Publication Giaime, E. et al. Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice. Neuron. 96, 796-807.e6 (2017)

Species mouse

Application WB

Publication Shimozawa, A. et al. Propagation of pathological α-synuclein in marmoset brain. Acta Neuropathol. Commun. 5, 12 (2017)

Species monkey

Application ICC-IF

Publication Sergin, I. et al. Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosis. Nat. Commun. 8, 15750 (2017).

Species human,mouse

Application WB,IHC (frozen),ICC-IF

Publication Conte, A. et al. High mobility group A1 protein modulates autophagy in cancer cells. Cell.Death.Differ. 24, 1948-1962 (2017).

Species human

Application ICC-IF

Publication Nascimbeni, A. C., Fanin, M., Angelini, C. & Sandri, M. Autophagy dysregulation in Danon disease. Cell Death Dis. 8, e2565 (2017).

Species human

Application WB,IHC (frozen)

Publication Gallagher, L. et al. Lysosomotropism depends on glucose: a chloroquine resistance mechanism. Cell.Death.Dis. 8, e3014 (2017).

Species mouse

Application WB

Publication Sample, A. et al. Adaptor protein p62 promotes skin tumor growth and metastasis and is induced by UVA radiation. J.Biol.Chem. 292, 14786-14795 (2017).

Species human

Application WB

Publication Nascimbeni, A. et al. Local detection of PtdIns3P at autophagosome biogenesis membrane platforms. Autophagy. 13, 1602-1612 (2017).

Species human

Application WB

Publication Yanagisawa, H. et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci.Rep. 7, 15944 (2017).

Species human

Application WB

Publication Feldmann, A. et al. The RAB GTPase RAB18 modulates macroautophagy and proteostasis. Biochem. Biophys. Res. Commun. 486, 738–743 (2017).

Species human

Application WB,IHC (frozen)

Publication Gómez-Sintes, R. et al. Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells. 6, (2017)

Species mouse

Application ICC-IF

Publication Zhang, J. et al. A Founder Mutation in VPS11 Causes an Autosomal Recessive Leukoencephalopathy Linked to Autophagic Defects. PLoS Genet. 12,(2016).

Species human

Application WB

Publication Salas, E., Roy, S., Marsh, T., Rubin, B. & Debnath, J. Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene 35, 2913–2922 (2016).

Species mouse

Application ICC-IF

Publication Taneike, M. et al. mTOR Hyperactivation by Ablation of Tuberous Sclerosis Complex 2 in the Mouse Heart Induces Cardiac Dysfunction with the Increased Number of Small Mitochondria Mediated through the Down-Regulation of Autophagy. PLoS One 11, (2016).

Species mouse

Application WB

Publication Angelini, C., Nascimbeni, A. C., Cenacchi, G. & Tasca, E. Lipolysis and lipophagy in lipid storage myopathies. Biochim. Biophys. Acta 1367–1373 (2016).

Species human

Application WB,IHC (frozen)

Publication Kaizuka, T. & Mizushima, N. Atg13 Is Essential for Autophagy and Cardiac Development in Mice. Mol. Cell. Biol. 36, 585–595 (2016).

Species mouse

Application WB,ICC-IF

Publication Santanam, U. et al. Atg7 cooperates with Pten loss to drive prostate cancer tumor growth. Genes Dev. 30, 399–407 (2016).

Species mouse

Application WB

Publication Yamanaka, T. et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Nat. Publ. Gr. 6, (2016).

Species mouse

Application WB,IHC (frozen)

Publication Saito, T. et al. p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,IHC (paraffin)

Publication Puvirajesinghe, T. M. et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2–JNK signalling in breast cancer. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,ICC-IF

Publication Venkatesh, A., Ma, S. & Punzo, C. TSC but not PTEN loss in starving cones of retinitis pigmentosa mice leads to an autophagy defect and mTORC1 dissociation from the lysosome. Cell Death Dis. 7, e2279–e2279 (2016).

Species mouse

Application IHC-IF

Publication Klionsky, D. J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12, 1 (2016).

Species mouse

Application WB

Publication Ra, E. A. et al. TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells. Nat. Commun. 7, (2016).

Species human

Application WB

Publication Kenific, C. M. et al. NBR1 enables autophagy-dependent focal adhesion turnover. J. Cell Biol. 212, 577–90 (2016).

Species human

Application WB

Publication Starheim, K. et al. Intracellular glutathione determines borte. cytotoxicity in multiple myeloma cells. Blood Cancer J. 6, (2016).

Species human

Application WB

Publication Baranowska, K. et al. Hydroxychloroquine potentiates carfilzomib toxicity towards myeloma cells. Oncotarget 7, 70845–70856 (2016)

Species human

Application WB

Publication Sergin, I. et al. p62-enriched inclusion bodies in macrophages protect against atherosclerosis HHS Public Access. Sci. Signal. 9, (2016).

Species mouse

Application IHC (frozen),ICC-IF

Publication Puvirajesinghe, T. M. et al. Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer. Nat. Commun. 7, (2016).

Species human,mouse

Application WB,ICC-IF

Publication Duleh, S., Wang, X., Komirenko, A. & Margeta, M. Activation of the Keap1/Nrf2 stress response pathway in autophagic vacuolar myopathies. Acta Neuropathol. Commun. 4, 115 (2016).

Species human,mouse

Application WB,IHC (frozen)

Publication Achour, I. et al. Oleuropein Prevents Neuronal Death, Mitigates Mitochondrial Superoxide Production and Modulates Autophagy in a Dopaminergic Cellular Model. Int. J. Mol. Sci. 17, 1293 (2016)

Species rat

Application WB

Publication Lim, S. W., Jin, L., Jin, J. & Yang, C. W. Effect of Exendin-4 on Autophagy Clearance in Beta Cell of Rats with Tacrolimus-induced Diabetes Mellitus. Nat. Publ. Gr. 6, (2016).

Species rat

Application WB,IHC (paraffin)

Publication Corcelle-Termeau, E. et al. Excess sphingomyelin disturbs ATG9A trafficking and autophagosome closure. Autophagy 12, 533–849 (2016)

Species mouse

Application WB

Publication Jarvela, T. S. et al. The neural chaperone proSAAS blocks α-synuclein fibrillation and neurotoxicity. Proc. Natl. Acad. Sci. U. S. A. (2016).

Species human

Application IHC (frozen)

Publication Lim, J. et al. Proteotoxic Stress Induces Phosphorylation of p62/SQSTM1 by ULK1 to Regulate Selective Autophagic Clearance of Protein Aggregates. PLoS One 11, (2015).

Species human,mouse

Application WB,ICC-IF

Publication McMillan, E. M. et al. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS One 10, e0119382 (2015).

Species rat

Application WB

Publication Morimoto, D. et al. The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates. Nat. Commun. 6, (2015).

Species mouse

Application WB,ICC-IF

Publication Ciuffa, R. et al. The selective autophagy receptor p62 forms a flexible filamentous helical scaffold. Cell Rep. 11, 748–58 (2015).

Species human

Application WB

Publication Pellegrini, C. et al. All-trans retinoic acid and rapamycin normalize Hutchinson Gilford progeria fibroblast phenotype. Oncotarget 6, 29914–29928 (2015).

Species human

Application WB

Publication Shi, J. et al. NBR1 is dispensable for PARK2-mediated mitophagy regardless of the presence or absence of SQSTM1. Cell Death Dis. 6, (2015).

Species human

Application WB

Publication Newton, P. T., Vuppalapati, K. K., Bouderlique, T. & Chagin, A. S. Pharmacological inhibition of lysosomes activates the MTORC1 signaling pathway in chondrocytes in an autophagy-independent manner. Autophagy 11, 1594–1607 (2015).

Species mouse,rat

Application WB

Publication Akizu, N. et al. Biallelic mutations in SNX14 cause a syndromic form of cerebellar atrophy and lysosome-autophagosome dysfunction HHS Public Access. Nat. Genet. 47, 528–534 (2015).

Species human

Application WB

Publication Arel-Dubeau, A.-M. et al. Cucurbitacin E Has Neuroprotective Properties and Autophagic Modulating Activities on Dopaminergic Neurons. Oxid. Med. Cell. Longev. 2014, 15 (2015).

Species rat

Application WB

Publication Hieke, N. et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy 11, 1471–1483 (2015).

Species mouse

Application WB,ICC-IF

Publication Eino, A. et al. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. J. Cell Sci. 128, 4453–61 (2015).

Species mouse

Application IHC (paraffin)

Publication Milan, E. et al. A plastic SQSTM1/p62-dependent autophagic reserve maintains proteostasis and determines proteasome inhibitor susceptibility in multiple myeloma cells. Autophagy 11, 1161–1178 (2015).

Species human

Application WB,ICC-IF

Publication Felzen, V. et al. Estrogen receptor α regulates non-canonical autophagy that provides stress resistance to neuroblastoma and breast cancer cells and involves BAG3 function. Cell Death Dis. 6, (2015).

Species human

Application ICC-IF

Publication Chandrachud, U. et al. Unbiased Cell-based Screening in a Neuronal Cell Model of Batten Disease Highlights an Interaction between Ca 2+ Homeostasis, Autophagy, and CLN3 Protein Function. J. Biol. Chem. 290, 14361–14380 (2015).

Species mouse

Application WB

Publication Johansson, I. et al. The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells. Autophagy 11, 1636–1651 (2015).

Species human,mouse

Application WB,ICC-IF

Publication Lenoir, O. et al. Endothelial cell and podocyte autophagy synergistically protect from diabetes-induced glomerulosclerosis. Autophagy 11, 1130–45 (2015).

Species mouse

Application WB

Publication Ruozi, G. et al. AAV-mediated in vivo functional selection of tissue-protective factors against ischaemia. Nat. Commun. 6, (2015).

Species mouse

Application WB

Publication Agarwal, S. et al. Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol- 3-phosphate-specific phospholipase A1 activity. Nat. Commun. 6, (2015).

Species human

Application WB

Publication Larroquette, F. et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum. Mol. Genet. 24, 6515–6529 (2015).

Species mouse

Application WB

Publication Liu, S. et al. Disrupted autophagy after spinal cord injury is associated with ER stress and neuronal cell death. Cell Death Dis. 6, (2015).

Species rat

Application WB,IHC (frozen)

Publication Echeverry, N. et al. Inhibition of autophagy sensitizes malignant pleural mesothelioma cells to dual PI3K/mTOR inhibitors. Cell Death Dis. 6, (2015).

Species human

Application WB

Publication Zhou, Z. et al. Autophagy supports color vision. Autophagy 11, 1821–1832 (2015).

Species mouse

Application IHC (frozen)

Publication Ulbricht, A. et al. Induction and adaptation of chaperone-assisted selective autophagy CASA in response to resistance exercise in human skeletal muscle. Autophagy 11, 538–546 (2015).

Species human

Application WB

Publication Murrow, L., Malhotra, R. & Debnath, J. ATG12-ATG3 Interacts with Alix to Promote Basal Autophagic Flux and Late Endosome Function. Nat. Cell Biol. 17, 300–310 (2015).

Species mouse

Application WB

Publication Lodder, J. et al. Macrophage autophagy protects against liver fibrosis in mice. Autophagy 11, 1280–1292 (2015).

Species mouse

Application ICC-IF

Publication Clayton, E. L. et al. Frontotemporal dementia caused by CHMP2B mutation is characterised by neuronal lysosomal storage pathology. Acta Neuropathol. 130, 511–23 (2015).

Species mouse

Application IHC (paraffin),IEM

Publication Xu, X. et al. Autophagy is essential for effector CD8 T cell survival and memory formation. Nat. Immunol. 15, 1152–1161 (2014).

Species mouse

Application WB

Publication Cottam, E. M., Whelband, M. C. & Wileman, T. Coronavirus NSP6 restricts autophagosome expansion. Autophagy 10, (2014).

Species monkey

Application WB,ICC-IF

Publication Lim, Y.-M. et al. Systemic autophagy insufficiency compromises adaptation to metabolic stress and facilitates progression from obesity to diabetes. Nat. Commun. 5, (2014).

Species mouse

Application WB

Publication Ricketts, T. et al. A nonsense mutation in mouse Tardbp affects TDP43 alternative splicing activity and causes limb-clasping and body tone defects. PLoS One 9, (2014).

Species mouse

Application IHC (paraffin)

Publication Karlsson, A. B. et al. The role of spartin and its novel ubiquitin binding region in DALIS occurrence. Mol. Biol. Cell 25, 1355–1365 (2014).

Species mouse

Application ICC-IF

Publication Costes, S., Gurlo, T., Rivera, J. F. & Butler, P. C. UCHL1 deficiency exacerbates human islet amyloid polypeptide toxicity in β-cells: evidence of interplay between the ubiquitin/proteasome system and autophagy. Autophagy 10, 1004–14 (2014).

Species mouse

Application WB,IHC (paraffin)

Publication Yamanaka, T. et al. NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization. Nat. Commun. 5, (2014).

Species mouse

Application WB,IHC (frozen),IEM

Publication Zhang, L. et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat. Commun. 5, (2014).

Species mouse

Application WB,IHC (paraffin),ICC-IF,IP

Publication Sarkar, C. et al. Impaired autophagy flux is associated with neuronal cell death after traumatic brain injury. Autophagy 10, 2208–2222 (2014).

Species mouse

Application IHC (frozen)

Publication Rivera, J. F., Costes, S., Gurlo, T., Glabe, C. G. & Butler, P. C. Autophagy defends pancreatic β cells from human islet amyloid polypeptide-induced toxicity. J. Clin. Invest. 124, 3489–500 (2014).

Species mouse

Application WB,IHC (paraffin)

Publication Dunlop, E. A. et al. FLCN, a novel autophagy component, interacts with GABARAP and is regulated by ULK1 phosphorylation. Autophagy 10, 1749–1760 (2014).

Species human,mouse

Application WB,IHC

Publication Sahani, M. H., Itakura, E. & Mizushima, N. Expression of the autophagy substrate SQSTM1/p62 is restored during prolonged starvation depending on transcriptional upregulation and autophagy-derived amino acids. Autophagy 10, 431–41 (2014).

Species mouse

Application WB,ICC-IF

Publication Furuya, N. et al. PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation. Autophagy 10, 631–41 (2014).

Species mouse

Application WB

Publication Heijnen, H. F. et al. Ribosomal Protein Mutations Induce Autophagy through S6 Kinase Inhibition of the Insulin Pathway. PLoS Genet. 10, (2014).

Species human

Application WB,ICC-IF

Publication Gabandé-Rodrıuez, E., Boya, P., Labrador, V., Dotti, C. & Ledesma, M. High sphingomyelin levels induce lysosomal damage and autophagy dysfunction in N324ann Pick disease type A. Cell Death Differ. 21, 864–875 (2014).

Species mouse

Application WB

Publication Kageyama, S. et al. Proteasome Dysfunction Activates Autophagy and the Keap1-Nrf2 Pathway. J. Biol. Chem. 289, 24944–24955 (2014).

Species mouse

Application WB,IHC (frozen)

Publication Kondratskyi, A. et al. Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death. Cell Death Dis. 5, (2014).

Species human

Application WB

Publication Kinsey, C. et al. Plac8 links oncogenic mutations to regulation of autophagy and is critical to pancreatic cancer progression. Cell Rep. 7, 1143–1155 (2014).

Species human,mouse

Application WB

Publication Nicot, A.-S. et al. Phosphorylation of NBR1 by GSK3 modulates protein aggregation. Autophagy 10, 1036–53 (2014).

Species human

Application WB

Publication Khan, M. M. et al. Role of autophagy, SQSTM1, SH3GLB1, and TRIM63 in the turnover of nicotinic acetylcholine receptors. Autophagy 10, 123–36 (2014).

Species mouse

Application WB,IHC

Publication Ost, M., Werner, F., Dokas, J., Klaus, S. & Voigt, A. Activation of AMPKa2 Is Not Crucial for Mitochondrial Uncoupling-Induced Metabolic Effects but Required to Maintain Skeletal Muscle Integrity. PLoS One 9, (2014).

Species mouse

Application WB

Publication Guan, B.-J. et al. Translational Control during Endoplasmic Reticulum Stress beyond Phosphorylation of the Translation Initiation Factor eIF2␣ *. J. Biol. Chem. 289, 12593–12611 (2014).

Species mouse

Application WB

Publication Tanaka, K. et al. A Novel Acylaminoimidazole Derivative, WN1316, Alleviates Disease Progression via Suppression of Glial Inflammation in ALS Mouse Model. PLoS One 9, (2014).

Species mouse

Application WB

Publication Zatloukal, B. et al. Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with mallory-denk bodies. PLoS One 9, (2014).

Species mouse

Application IHC (frozen)

Publication Shackelford, D. B. et al. LKB1 inactivation dictates therapeutic response of non-small cell lung cancer to the metabolism drug phenformin. Cancer Cell 23, 143–58 (2013).

Species human

Application WB,IHC

Publication Ozcelik, S. et al. Rapamycin Attenuates the Progression of Tau Pathology in P301S Tau Transgenic Mice. PLoS One 8, (2013)

Species mouse

Application WB

Publication Jiang, S., Guo, R., Zhang, Y., Zou, Y. & Ren, J. Heavy metal scavenger metallothionein mitigates deep hypothermia-induced myocardial contractile anomalies: role of autophagy. Am. J. Physiol. - Endocrinol. Metab. 304, (2013).

Species mouse

Application WB

Publication Schober, A. et al. Cell Loss and Autophagy in the Extra‐Adrenal Chromaffin Organ of Zuckerkandl are Regulated by Glucocorticoid Signalling. J. Neuroendocrinol. 25, 34–47 (2013)

Species mouse

Application ICC-IF

Publication Yamano, K. & Youle, R. J. PINK1 is degraded through the N-end rule pathway. Autophagy 9, 1758–69 (2013).

Species human

Application ICC-IF

Publication Kondylis, V. et al. Endosome-mediated autophagy: an unconventional MIIC-driven autophagic pathway operational in dendritic cells. Autophagy 9, 861–80 (2013).

Species mouse

Application WB,ICC-IF,IEM

Publication Yuan, H.-X., Russell, R. C. & Guan, K.-L. Regulation of PIK3C3/VPS34 complexes by MTOR in nutrient stress-induced autophagy. Autophagy 9, 1983–95 (2013).

Species human,mouse

Application WB

Publication Spilman, P. et al. Inhibition of mTOR by Rapamycin Abolishes Cognitive Deficits and Reduces Amyloid-β Levels in a Mouse Model of Alzheimer’s Disease. PLoS One 5, e9979 (2010).

Species mouse

Application WB

Publication Waguri, S. & Komatsu, M. Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice. Methods in Enzymology 453, 181–196 (2009).

Species mouse

Application WB,IHC (frozen)

Downloads

File

Category

Size

Filetype

File IHC guinea pig antibody

Category Protocol

Size 167.92 KB

Filetype pdf

File WB protocol

Category Protocol

Size 210.69 KB

Filetype pdf

Datasheet

Q & A's

Please enter a valid email address.

Your question *

Please enter a valid question.

I agree that my data will be processed to answer the question. I have taken note of the Privacy Policy and agree to the information described therein.*

Fields marked with * are mandatory.

There aren't any asked questions yet.

Customer Reviews

0 of 0 reviews

Leave a review!

Share your experiences with other customers.

Login

Your email address

Your password

Display reviews in current language only.

No reviews found. Be the first to share your insights.

FAQs

PVDF membranes show better results than nitrocellulose (higher capacity, allows for more stringent washing conditions in case of background problems).
Use freshly prepared blocking solution (e.g. 5% nonfat dry milk, 0.05% Tween 20), block for at least 1 h at room temperature.
Use the antibody in a higher dilution, but prolong incubation time and exposure time.
Always use a fresh aliquot of the antibody.
Do not repeatedly freeze the antibody (eventually centrifuge shortly after thawing to remove cryo-precipitates).
Include an additional washing step.
You might also try more stringent wash conditions, e.g. add 0.5 M NaCl to the wash buffer.
Always use a fresh aliquot of secondary antibody.
In case you use ECL most the guinea pig antibody should be diluted further in order to get rid of the background.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

The concentration of specific antibody in our guinea pig serum is not determined.
In guinea pigs the antibody concentration in serum varies from 10 to 20 mg/ml; specific antibodies represent normally about 0.1-1% of total IgG. Total protein concentration varies from 40 to 65 mg/ml, with the main constituent (about 60%) being albumin.

Progen Support

For scientific and customer support, you can reach us by phone or email.