A) Dot blot analysis showing the comparison of cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, cat. number 690383) with a multi-serotype recognizing AAV antibody from another vendor. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 1 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to verify binding of the antibody to conformational epitopes. Please note that the two antibody experiments were carried out on separate occasions with different secondary antibodies, which could impact the reliability of direct dot blot comparison.
B) Cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, cat. number 690383) with AAV serotypes and variants, using dot blot analysis. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 0.2 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to exclude binding to non-assembled capsids.
A) Dot blot analysis showing the comparison of cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, cat. number 690383) with a multi-serotype recognizing AAV antibody from another vendor. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 1 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to verify binding of the antibody to conformational epitopes. Please note that the two antibody experiments were carried out on separate occasions with different secondary antibodies, which could impact the reliability of direct dot blot comparison.
B) Cross-reactivity of the anti-pan AAV (mouse chimeric antibody, IgG2a, cat. number 690383) with AAV serotypes and variants, using dot blot analysis. A concentration of 0.1 ug/ml of the anti-pan AAV antibody and 0.2 ug/ml of the AAV antibody from the alternative vendor was used. Additionally, a total amount of 1E+10 capsids was applied. Denatured capsids were used as a control, to exclude binding to non-assembled capsids.
Key Features
- Monoclonal recombinant mouse chimeric antibody
- Suitable for dot blot, IF, ELISA and neutralization
- Reacts with AAV1-9, AAV11, AAV13, AAVrh10, AAVrh74, Anc80L65, AAV2.7m8, AAVLK03, AAVhu.37, AAVmyo, AAV4A, AAV4E, AAV-PHP.eB, AAV-F, AAVp-P5, scAAV9 intact particles
- Isotype: mouse IgG2a
Product description
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Recombinant chimeric (mouse Fc region) |
| Isotype | IgG2a |
| Conjugate | Unconjugated |
| Application | Dot blot, ELISA, ICC/IF, Neutralization assay |
| Purification | Affinity chromatography |
| Reactivity | AAV-F, AAV-LK03, AAV-PHP.eB, AAV1, AAV2, AAV2.7m8, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV9-P5, AAV11, AAV13, AAVAnc80L65, AAVDJ, AAVhu.37, AAVmyo, AAVmyo4A, AAVmyo4E, AAVrh10, AAVrh74, scAAV9 |
| Storage | 2-8°C |
| Intended use | Research use only |
| Clone | 1C4M |
| Immunogen | AAV capsids |
| Concentration | 50 µg/ml |
| Formulation | PBS, pH 7.4 with 0.09% sodium azide and 0.5% BSA |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:20-1:50 (1-2.5 µg/ml) |
| Dot Blot | 1:500 (0.1 µg/ml; non-denaturing conditions) |
| ELISA | Assay dependent |
| Neutralization Assay | EC50 ~ 7 ng/ml (AAV1), ~ 195 ng/ml (AAV3), ~ 248 ng/ml (AAV5), ~ 8 ng/ml (AAV6), ~ 52 ng/ml (AAV8), ~ 27 ng/ml (AAV9), ~ 441 ng/ml (AAVrh10), assay dependent |
Background
The anti-pan AAV is a recombinant mouse chimeric antibody of human origin, specifically recognizing a conformational epitope only present on fully assembled and intact AAV capsids. It does not detect denatured AAV capsids, making it a versatile tool for various applications, such as tracking the localization of viral particles, studying protein-protein interactions, facilitating AAV particle purification, and determining total AAV capsid titers. Additionally, the antibody exhibits neutralizing activity against multiple AAV serotypes. The anti-pan AAV demonstrates broad binding capabilities across all AAV serotypes, with the exception of AAV12. Furthermore, it binds to numerous additional AAV variants, underscoring its versatility in diverse research and quality control settings.
Limited Use Label License: Research Use Only
Product is exclusively owned by PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.
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