anti-p0071 mouse monoclonal, 7.7.9, supernatant

p0071 staining on human kidney (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science) View more

Cat. No: 651165

Prices excl. tax

Available, delivery time 1-7 days

$251.00*

Datasheet

Key Features

Mouse monoclonal
Suitable for ICC/IF, IHC and WB
Reacts with bovine and human
Isotype: IgG1

Product description

Quantity	5 ml
Antibody Type	Monoclonal
Host	Mouse
Isotype	IgG1
Conjugate	Unconjugated
Application	ICC/IF, IHC, WB
Purification	Hybridoma cell culture supernatant
Reactivity	Bovine, Human
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Clone	7.7.9
Immunogen	Synthetic peptide of human p0071 (aa position 85-104: EKSFPWRSTDVNTGVSKPR)
Formulation	Contains 0.09% sodium azide
UniprotID	Q99569 (Human)
Synonym	Plakophilin-4, p0071, PKP4

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	Ready-to-use
Immunohistochemistry (IHC) - frozen	Ready-to-use
Immunohistochemistry (IHC) - paraffin	Ready-to-use (microwave treatment recommended)
Western Blot (WB)	1:10-1:50

Background

Detects a plaque protein (p0071) specific for a subtype of adherens junctions, with a molecular mass of 135 kDa and is closely related to neurojungin / delta-catenin and ARVC (all belonging to the p120 armadillo subfamily). p0071 is specifically detected in the plaques of the composite junctions (area compositae) of cardiac intercalated disks, in puncta adhaerentia of single- and multi-layered epithelia and blood vessel endothelial cells.; it is, however, absent from desmosomes.
This protein was also described as plakophilin 4.
Reactivity on cultured cell lines: MCF-7, A431, CaCo-2, HT-29, PLC, 16HBE14o, HaCaT, SV80, U333 CG, 3T3, RVF-SMC, MH1C1
Positive control: MCF-7 cells

References/Publications (3)

Publication

Species

Application

Publication Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

Species pig

Application ICC-IF

Publication Hofmann, I., Schlechter, T., Kuhn, C., Hergt, M. & Franke, W. W. Protein p0071 - an armadillo plaque protein that characterizes a specific subtype of adherens junctions. J. Cell Sci. 122, 21–24 (2009).

Species human

Application WB,IHC (frozen)

Publication Hofmann, I., Kuhn, C. & Franke, W. W. Protein p0071, a major plaque protein of non-desmosomal adhering junctions, is a selective cell-type marker. Cell Tissue Res. 334, 381–399 (2008).

Species human

Application IHC (frozen),IEM

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Datasheet

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FAQs

The concentration of unpurified supernatant, ascites, unpurified guinea pig serum and unpurified rabbit serum is not determined.
The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at support@progen.com.

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

PROGEN offers different formats for mouse monoclonal antibodies. Different antibodies may not be offered in each format, the following are all possible formats:

Supernatant and supernatant concentrate: This format contains hybridoma cell culture supernatant. The antibody is not purified and the antibody concentration is not determined. The antibody concentration may vary from lot to lot. Therefore we recommend to titrate the optimal concentration for the application used for each new lot.
Lyophilized, purified: This format contains purified antibody in lyophilized form. The reconstitution of this antibody is described in the datasheet. The buffer composition after reconstitution is also mentioned on the datasheet.
Liquid, purified: This format contains purified antibody in liquid format. The concentration is mentioned on the datasheet.
Prediluted, purified: This format contains purified antibody in liquid format. Most antibodies in this format are diluted to be ready-to-use for IHC with standard tissue. But some antibodies of this format need further dilution for IHC. This is mentioned on the datasheet.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

We offer bulk amounts starting from 1 mg for many of our mouse monoclonal or recombinant antibodies. Please contact us for further information or use the “bulk button” on the product page.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.

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anti-p0071 mouse monoclonal, 7.7.9, supernatant

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