anti-Mucin 2 mouse monoclonal, MUC-2/Ccp58, supernatant
- Mouse monoclonal
- Suitable for IHC on frozen and paraffin sections
- Reacts with human
- Isotype: IgG1
|Purification||Hybridoma cell culture supernatant|
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic human MUC-2 (MI-29) peptide: MUC2 MI-29 (KYPTTTPISTTTMVTPTPTPTGTQTPTTT)|
|Formulation||Contains 0.09% sodium azide|
|Synonym||Mucin-2, MUC-2, Intestinal mucin-2, MUC2, SMUC|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:10-1:40|
|Immunohistochemistry (IHC) - paraffin||1:10-1:40 (microwave treatment recommended)|
The antibody reacts with mucin from small and large intestine and to a weaker extent with salivary and breast epithelia. Stomach, pancreas, kidney, and ovary epithelia are negative. Positive reaction of gastric cancer and colon cancer (antibody shows a relative high specificity for colon carcinoma).
Reactivity on cultured cell lines: LS 174 T (colon cancer cell line).
Positive control: Small intestine.
Q & A's
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The concentration of purified antibodies is mentioned on the datasheet.
For prediluted antibodies the concentration may vary from lot to lot. The concentration of these antibodies is not mentioned on the datasheet and can be requested at email@example.com.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.