protag-HiPur GFP Agarose Beads

A) Pull-down of GFP from a mixture of GFP, mCherry and mTagBFP. B) IP of GFP from HeLa lysate. In/Ft: 1/1000 of input and non-bound material. E: Eluate from 1 µl of beads. C) Control experiment using functionalized beads lacking sdAbs. View more

Cat. No: 89100L

Prices excl. VAT and shipping costs

Available, delivery time 1-7 days

$663.00*

Datasheet

Key Features

Camelid single-domain antibody (sdAb) covalently linked to 4% agarose beads.

Product description

Quantity	2 ml
Antibody Type	Recombinant, produced in E.coli
Host	Llama/alpaca
Isotype	Single-domain antibody
Application	IP
Purification	Affinity chromatography
Reactivity	Cerulean, Citrine, EGFP, Emerald GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire
No reactivity	Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Storage	2-8°C
Intended use	Research use only
Clone	1H1
Immunogen	GFP
Formulation	50% slurry in PBS containing 20% Ethanol
Capacity	> 4 µg GFP per µl of packed beads
Coating	sdAb anti-GFP clone 1H1
Support	4% cross-linked agarose, bead size 50-150 µm
Buffer compatibility	Common buffer substances at pH 5 to 9 2% Triton X-100, 1% Tween-20, 1% NP-40, 1% CHAPS, 1% Deoxycholate, 0.1% SDS 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA 4 M urea 10 mM DTT, 10 mM 2-Mercaptoethanol RNAse A, DNAse I, Benzonase, protease inhibitors

Applications

Tested applications	Tested dilutions
Immunoprecipitation (IP)	yes

Background

protag-HiPur GFP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur GFP Agarose Beads thus feature high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. protag-HiPur GFP Agarose Beads immobilize a wide range of GFP derivatives, such as GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. protag-HiPur GFP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur GFP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.

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Category Protocol

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Datasheet

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