protag-HiPur GFP Agarose Beads
Key Features
Camelid single-domain antibody (sdAb) covalently linked to 4% agarose beads.
Product description
Quantity | 2 ml |
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Antibody Type | Recombinant, produced in E.coli |
Host | Llama/alpaca |
Isotype | Single-domain antibody |
Application | IP |
Purification | Affinity chromatography |
Reactivity | Cerulean, Citrine, EGFP, Emerald GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire |
No reactivity | Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives |
Storage | 2-8°C |
Intended use | Research use only |
Clone | 1H1 |
Immunogen | GFP |
Formulation | 50% slurry in PBS containing 20% Ethanol |
Capacity | > 4 µg GFP per µl of packed beads |
Coating | sdAb anti-GFP clone 1H1 |
Support | 4% cross-linked agarose, bead size 50-150 µm |
Buffer compatibility |
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Applications
Tested applications | Tested dilutions |
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Immunoprecipitation (IP) | yes |
Background
protag-HiPur GFP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur GFP Agarose Beads thus feature high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. protag-HiPur GFP Agarose Beads immobilize a wide range of GFP derivatives, such as GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. protag-HiPur GFP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur GFP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.
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