protag-HiPur MBP Agarose Beads
Immunoprecipitation of MBP from HeLa lysate. In/Ft: 1/1000 of input and non-bound material. E: Eluate from 1 µl of beads. Right panel: Control experiment using functionalized beads lacking sdAbs. View more
Camelid single-domain antibody (sdAb) covalently linked to 4% agarose beads.
|Antibody Type||Recombinant, produced in E.coli|
|Intended use||Research use only|
|Immunogen||MBP (E. coli maltose-binding protein)|
|Formulation||50% slurry in PBS containing 20% Ethanol|
|Capacity||> 2.5 µg MBP per µl of packed beads|
|Coating||sdAb anti-MBP clone 1G5|
|Support||4% cross-linked agarose, bead size 50-150 µm|
|Tested applications||Tested dilutions|
protag-HiPur MBP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur MBP Agarose Beads thus feature high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background. protag-HiPur MBP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur MBP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.
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