protag-HiPur RFP Agarose Beads
A) Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP. B) IP of mCherry from HeLa lysate. In/Ft: 1/1000 of input and non-bound material. E: Eluate from 1 µl of beads. C) Control experiment using functionalized beads lacking sdAbs. View more
Camelid single-domain antibody (sdAb) covalently linked to 4% agarose beads.
|Antibody Type||Recombinant, produced in E.coli|
|Reactivity||dsRed1/dsRed2, mCherry, mOrange2, mRFP, mScarlet-i, tdTomato|
|No reactivity||Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, GFP, mTagBFP or their most common derivatives|
|Intended use||Research use only|
|Formulation||50% slurry in PBS containing 20% Ethanol|
|Capacity||> 4 µg RFP per µl of packed beads|
|Coating||sdAb anti-RFP clone 2B12|
|Support||4% cross-linked agarose, bead size 50-150 µm|
|Tested applications||Tested dilutions|
protag-HiPur RFP Agarose Beads are based on a high-affinity single-domain antibodies (sdAb) that are covalently immobilized on 4% cross-linked agarose beads. The sdAbs are attached via a flexible linker which guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. protag-HiPur RFP Agarose Beads thus feature high affinity and superior capacity for RFP fusion proteins while showing negligible non-specific background. protag-HiPur GFP Agarose Beads immobilize a wide range of RFP derivatives, such as most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato and mScarlet. It does not cross-react with GFP or mTagBFP/mTagRFP derivatives. protag-HiPur RFP Agarose Beads are compatible not only with physiological buffers but also with high stringency buffers. With protag-HiPur RFP Agarose Beads the binding and washing conditions can be adjusted to the experimental needs.
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