protag-HiRes anti-GFP-X1 Alexa 647
- Alexa 647 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with acGFP, Cerulean, Citrine, eCFP, GFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
|Antibody Type||Recombinant, produced in E.coli|
|Reactivity||Cerulean, Citrine, EGFP, Emerald GFP, GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire|
|No reactivity||Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives|
|Storage||Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!|
|Intended use||Research use only|
|Formulation||5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunocytochemistry (ICC)/ Immunofluorescence (IF)||1:500|
protag-HiRes anti-GFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, GFP, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.
In protag-HiRes anti-GFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Q & A's
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The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.