protag-HiRes anti-GFP-X1 Alexa 647
Immunostaining of PFA fixed 3T3 cells expressing a TOM70-GFP reporter protein with protag-HiRes anti-GFP-X1Atto 647N (dilution 1:500, the GFP signal is represented in green, the corresponding protag-HiRes signal is represented in red and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Stable HeLa cell line expressing YFP-fusion protein (A). Cells were stained with protag-HiRes anti-GFP-X1 Atto 488 (B, Cat. No. 81105) and protag-HiRes anti-GFP-X4 Atto 488 (C, Cat. No. 81405L)(courtesy of NanoTag Biotechnologies GmbH).
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Stable HeLa cell line expressing YFP-fusion protein (A, false color). Cells were stained with protag-HiRes anti-GFP-X1 Atto 542 (B, false color). Overlay of both channels and additional DAPI stain (C)(courtesy of NanoTag Biotechnologies GmbH)
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Immunostaining of PFA fixed 3T3 cells expressing a TOM70-GFP reporter protein with protag-HiRes anti-GFP-X1Atto 647N (dilution 1:500, the GFP signal is represented in green, the corresponding protag-HiRes signal is represented in red and the merge of both channels is represented in yellow). Nuclei were visualized by DAPI staining (blue)(courtesy of NanoTag Biotechnologies GmbH).
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Stable HeLa cell line expressing YFP-fusion protein (A). Cells were stained with protag-HiRes anti-GFP-X1 Atto 488 (B, Cat. No. 81105) and protag-HiRes anti-GFP-X4 Atto 488 (C, Cat. No. 81405L)(courtesy of NanoTag Biotechnologies GmbH).
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Stable HeLa cell line expressing YFP-fusion protein (A, false color). Cells were stained with protag-HiRes anti-GFP-X1 Atto 542 (B, false color). Overlay of both channels and additional DAPI stain (C)(courtesy of NanoTag Biotechnologies GmbH)
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Key Features
- Alexa 647 conjugate
- Llama/alpaca single-domain antibody
- Suitable for ICC/IF
- Reacts with acGFP, Cerulean, Citrine, eCFP, GFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
Product description
| Quantity | 200 µl |
|---|---|
| Antibody Type | Recombinant, produced in E.coli |
| Host | Llama/alpaca |
| Isotype | Single-domain antibody |
| Conjugate | Alexa 647 |
| Application | ICC/IF |
| Purification | Affinity chromatography |
| Reactivity | Cerulean, Citrine, EGFP, Emerald GFP, GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire |
| No reactivity | Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives |
| Storage | Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light! |
| Intended use | Research use only |
| Clone | 1H1 |
| Immunogen | GFP |
| Formulation | 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide. |
| Note | Centrifuge prior to opening |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:500 |
Background
protag-HiRes anti-GFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, GFP, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.
In protag-HiRes anti-GFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
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FAQs
Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.
Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.
The expiration date of our antibodies is indicated on the product label.
Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.
The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.
PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.