protag-HiRes anti-GFP-X1 Sulfo-Cyanin 5 (Cy5)

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Cat. No: 81110L

Available, delivery time 1-7 days

$504.00*

Key Features
  • Sulfo-Cyanin 5 (Cy5) conjugate
  • Llama/alpaca single-domain antibody
  • Suitable for ICC/IF
  • Reacts with acGFP, Cerulean, Citrine, eCFP, EGFP, Emerald GFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, Sirius, superecliptic pHluorin, superfold GFP, tSapphire
Product description
Quantity 200 µl
Antibody Type Recombinant, produced in E.coli
Host Llama/alpaca
Isotype Single-domain antibody
Conjugate Sulfo-Cyanin 5 (Cy5)
Application ICC/IF
Purification Affinity chromatography
Reactivity Cerulean, Citrine, EGFP, Emerald GFP, Sirius, Superecliptic pHluorin, Superfold GFP, acGFP, eCFP, eYFP, mEGFP (A206K), mEGFP (L221K), mTurquoise 1/2, mVenus, paGFP, tSapphire
No reactivity Dendra2, Dronpa, tdEOS, mEOS3.2, mRuby3, mTFP, mCherry, mRFP, dsRed, mTagBFP or their most common derivatives
Storage Up to 3 months: -20°C; up to 12 months: -80°C or below; protect from light!
Intended use Research use only
Clone 1H1
Immunogen GFP
Formulation 5 µM fluorescently labeled single-domain antibody in buffered saline, 50% glycerol, 0.09% sodium azide.
Note Centrifuge prior to opening
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:1,000
Background

protag-HiRes anti-GFP camelid single-domain antibody (sdAb) produced by NanoTag Biotechnologies GmbH. It recognizes GFP and its most common derivatives with high affinity and specificity. It recognizes Sirius, tSapphire, Cerulean, eCFP, mTurquoise 1/2, acGFP, EGFP, Emerald GFP, mEGFP (A206K), mEGFP (L221K), superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus, Citrine.

In protag-HiRes anti-GFP-X1, each fluorophore is coupled to exactly one single-domain antibody, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Downloads
File
Category
Size
Filetype
File ICC/IF
Category Protocol
Size 805.06 KB
Filetype pdf
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